47results about How to "Efficient and convenient production" patented technology

The present invention comprises a method for producing libraries of expressible gene sequences. The method of the invention allows for the simultaneous manipulation of multiple gene sequences and thus allows libraries to be created in an efficient and high throughput manner. The expression vectors containing verified gene sequences can be used to transfect cells for the production of recombinant proteins. The invention further comprises libraries of expressible gene sequences produced using the method of the invention and expression vectors used in the construction of said libraries.

This invention provides a method for producing recombinant human proinsulin from N-end leader peptide, and relevant expression vector. The method comprises: (1) fusing yeast alpha-factor leader peptide sequence and the human proinsulin N-end leader peptide, and introducing into a yeast constitutive expression vector; (2) transferring into Pichia pastoris cells to realize constitutive and secretory expression; (3) fermenting and purifying. The recombinant human proinsulin has such advantages as high stability, high activity and simple production. The method has such advantages as high expression level, high purification yield, and high efficiency.

The invention discloses fusion protein containing a leucine-rich repetitive sequence, and a preparation method and application thereof. Concretely, the invention relates to fusion protein, and the fusion protein possesses the following structure from N terminal to C terminal: A-X-E-Y1-Y2 (formula Ia) or A-Y2-Y1-E-X (formula Ib), wherein A is an optional secretion signal peptide, X is a member rich in leucine repetitive sequence 1-2 (LRRs1-2) of Slit2 protein (neuronal guidance factor 2), E is a restriction enzyme cutting site, Y1 is a first tag peptide member, Y2 is a second tag short peptide member and the second tag short peptide member is His tag member, and '-' represents a peptide bond or a peptide joint for connecting the above members. The fusion protein is beneficial for correct folding of LRR (leucine-rich repeat) and forming active functional polypeptide, and is capable of simply removing two tag members through once resection enzyme cutting, thereby preparing high-purity high-activity LRR sequence.

Provided is a new production method for the synthesis of an NC type nucleoside efficiently and conveniently in a high yield without unnecessary protecting group conversion steps. It relates to a step of producing a compound represented by the formula (II):or a salt thereof, by inverting a compound represented by the formula (I):and a method of producing a compound represented by the formula (III):or a salt thereof (wherein each symbol is as defined in the specification), which includes the step.

The invention discloses a high-speed structural steel intelligent production line, and relates to the technical field of intelligent control automation. In order to solve the problems that a high-speed structural steel production line is many in production working procedure, high in manual cost and low in work efficiency, the production line is provided. A flattening machine is arranged on one side of a dual-head uncoiling machine, an automatic shearing butt welding machine is arranged on the other side of the flattening machine, pinch devices are arranged on the two ends of the automatic shearing butt welding machine, a shear welding device is arranged in the middle of the automatic shearing butt welding machine, a horizontal spiral loop is arranged on the other side of the automatic shearing butt welding machine, a punching mechanism is arranged on the other side of the horizontal spiral loop, a forming main engine is arranged on the other side of the punching mechanism, a hydrauliccutting-off mechanism is arranged on the other side of the forming main engine, a stacking mechanism is arranged on the other side of the hydraulic cutting-off mechanism, a packaging mechanism is arranged on the other side of the stacking mechanism, and a full-automatic packaging machine is arranged at one end of the packaging mechanism.

The invention discloses fusion protein containing a leucine-rich repetitive sequence, and a preparation method and application thereof. Concretely, the invention relates to fusion protein, and the fusion protein possesses the following structure from N terminal to C terminal: A-X-E-Y1-Y2 (formula Ia) or A-Y2-Y1-E-X (formula Ib), wherein A is an optional secretion signal peptide, X is a member rich in leucine repetitive sequence 2 (LRRs2) of Slit2 protein (neuronal guidance factor 2), E is a restriction enzyme cutting site, Y1 is a first tag peptide member, Y2 is a second tag short peptide member and the second tag short peptide member is His tag member, and '-' represents a peptide bond or a peptide joint for connecting the above members. The fusion protein is beneficial for correct folding of LRR (leucine-rich repeat) and forming active functional polypeptide, and is capable of simply removing two tag members through once resection enzyme cutting, thereby preparing high-purity high-activity LRR sequence.

The invention relates to the electric wire and electric cable manufacture field, and particularly to a plastic extruding mould required by production of a concentric sparse winding shielding layer of a plurality of single-wire conductors under a cable sheath layer. Disclosed is the plastic extruding mould for producing electric cables. The plastic extruding mould includes a plastic extruding mould base; a male mould and a female mould are respectively arranged at two sides of a cavity in the plastic extruding mould base, a mould cavity is formed between the male mould and the female mould, the female mould is connected with a forming pipe, and the male mould is connected with a mould body; the interior of the mould body is connected with a stranding cage through bearings, the interior of the stranding cage is provided with a threading pipe, the stranding cage can rotate around the threading pipe, the right end of the stranding cage is provided with a distributing terminal board, and the distributing terminal board is provided with wire harness conduits. A wire core is passed through the threading pipe, then through the male mould and the female mould, next through the forming pipe, and is connected with a traction machine set; and sparse winding single wires are passed through the wire harness conduits and the distributing terminal board.

The invention discloses fusion protein containing a leucine-rich repetitive sequence, and a preparation method and application thereof. Concretely, the invention relates to fusion protein, and the fusion protein possesses the following structure from N terminal to C terminal: A-X-E-Y1-Y2 (formula Ia) or A-Y2-Y1-E-X (formula Ib), wherein A is an optional secretion signal peptide, X is a member rich in leucine repetitive sequence 2 (LRRs2) of Slit3 protein (neuronal guidance factor 3), E is a restriction enzyme cutting site, Y1 is a first tag peptide member, Y2 is a second tag short peptide member and the second tag short peptide member is His tag member, and '-' represents a peptide bond or a peptide joint for connecting the above members. The fusion protein is beneficial for correct folding of LRR (leucine-rich repeat) and forming active functional polypeptide, and is capable of simply removing two tag members through once resection enzyme cutting, thereby preparing high-purity high-activity LRR sequence.

The present invention relates to a method of synthesizing isoflavan and isoflavene derivatives of the Formula 1, which have the biological efficacy of antioxidation and protection against ultraviolet light. The method is effective and suitable for a mass production of isoflavan and isoflavene derivatives, which is a more convenient alternative preparation method compared to the isolation method by the extraction of plants, such as licorice, via troublesome preparative processes.

The present invention discloses car mat structures used to set on the floor compartment area in a car to contain water drops or debris fallen or brought into the car. One car mat structure includes a bottom pad with plural sidelines; and plural dams, each being raised from one said sideline, wherein two opposite members of the dams are dual slop walls each being built with two inclined surfaces with different inclined angles. Another car mat structure includes a bottom pad; plural dams raised from the sidelines of the bottom pad; and plural triangle shutters formed to cover bottom corners each formed by two adjacent dams and the bottom pad. A third car mat structure includes a bottom pad; plural dams raised from the sidelines of the bottom pad, wherein two opposite members of the dams each being built with two inclined surfaces with different inclined angles; and plural triangle shutters. With the implementation of the new design dams and triangle shutters of the present invention, molding is easier when forming and producing mat structures; cleaning becomes easier since no hidden corners existing on the mat structures; and deformation or collapsing of mat structures being effectively prevented. When further build an inclined cover pad to cover the junction area between a dam and the bottom pad, molding for production and cleaning the mats become even more convenient and efficient.

The present invention relates to a gene construct which is capable of achieving efficient production of an antimicrobial peptide in a microorganism, and a method for efficient mass production and separation of an antimicrobial peptide using the same. The gene construct of the present invention has a translationally coupled configuration of two independent and separate cistrons which encode an acidic peptide and a basic antimicrobial peptide, each having an opposite charge, under the control of a single promoter. The translationally coupled acidic peptide and basic antimicrobial peptide undergo charge-charge interaction simultaneously with expression thereof to neutralize the potential cytotoxicity of the antimicrobial peptide, resulting in prevention of antimicrobial peptide-mediated killing of host microorganisms. In addition, a conjugate of the acidic peptide and the antimicrobial peptide can be separated without chemical or enzymatic treatment. Therefore, it is possible to achieve easy mass production of antimicrobial peptides from recombinant microorganisms.

The invention provides a method of producing mutant of tumour necrosis factor called TNF-alphaand the related technology about expression carrying agent and, the construction, expression and decontamination of engineering cell. The protein gene of mutant of tumour necrosis factor called TNF-alpha,which has been optimized, is very much suitable for expression in pichia, meanwhile, improves expression quantity and the rate of purification through the optimization of fermentation and decontamination, has the advantages of high expression, good stability, compact production technology. This invention can get the protein gene of mutant of tumour necrosis factor called TNF-alpha with performance, briefness and low cost.

The invention relates to a reconstruction interferon gamma nucleotide sequence and the manufacture method to produce reconstruction interferon gamma, and the relative expression carrier and engineer cell construction, expression and purification technology. The method improves the expression quantity and the purification yield. It has the advantage of high expression, good stability, and simple technology. It could gain reconstruction human interferon gamma high efficiently, conveniently and in low cost.

The invention discloses a water-hydraulic ultrahigh pressure system for liquid food and a production device thereof, and belongs to the field of liquid food processing. The ultrahigh pressure system comprises a water-hydraulic system, a liquid food delivering, pressurizing, and pressure-maintaining system, and a filling system, which are connected in sequence. The water-hydraulic system provides basic work power to the whole system, the liquid food is subjected to an ultrahigh pressure treatment in the liquid food delivering, pressurizing, and pressure-maintaining system, then the processed liquid food is automatically extracted from the pressure cylinder and filled, the ultrahigh pressure system can prominently improve the processing efficiency of liquid food, and the production process is clean and pollution-free. At the same time, the pressure cylinder used in the processing process has a simple structure, is convenient to operate, and is suitable for the ultrahigh pressure system.

A rapid beverage cooling device and a refrigeration method thereof are provided. The rapid beverage cooling device includes an outer cup body and an inner cup body of which rims are connected. An interlayer inner cavity for storing a liquid is formed between the outer cup body and the inner cup body; a liquid injection port communicated with the interlayer inner cavity is made at a bottom of the outer cup body; the liquid injection port can be opened or sealed; a liquid accommodating cavity is disposed in the inner cup body; a convex column extends toward the liquid accommodating cavity from a wall of the inner cup body; and an inner cavity of the convex column is communicated with the interlayer inner cavity.

The invention discloses a production system of organic and inorganic compound fertilizer. The production system comprises a crusher, a quantitative feeding device, a material mixing machine, a fillingmachine and a bag sewing machine, wherein the crusher is provided with an organic fertilizer feeding opening and a discharging opening; the discharging opening is connected with a feeding opening formed in the top of the quantitative feeding device through a first elevator; a discharging opening of the quantitative feeding device is communicated with the material mixing machine; the material mixing machine is provided with a discharging opening and an inorganic fertilizer feeding opening; a discharging opening of the material mixing machine is connected with a feeding opening of the filling machine through a second elevator ; a discharging opening which is used for outputting through a spiral conveyor is formed in the bottom of the filling machine; the discharging opening is connected with the bag sewing machine. The production system of the organic and inorganic compound fertilizer, disclosed by the invention, has the advantages of simple technology, safety and high efficiency, highautomation degree, easiness for controlling and large-scale production; the production efficiency is improved and the labor intensity of workers is also reduced.

The invention discloses fusion protein containing a leucine-rich repetitive sequence, and a preparation method and application thereof. Concretely, the invention relates to fusion protein, and the fusion protein possesses the following structure from N terminal to C terminal: A-X-E-Y1-Y2 (formula Ia) or A-Y2-Y1-E-X (formula Ib), wherein A is an optional secretion signal peptide, X is a member rich in leucine repetitive sequence 1-2 (LRRs1-2) of Slit3 protein (neuronal guidance factor 3), E is a restriction enzyme cutting site, Y1 is a first tag peptide member, Y2 is a second tag short peptide member and the second tag short peptide member is His tag member, and '-' represents a peptide bond or a peptide joint for connecting the above members. The fusion protein is beneficial for correct folding of LRR (leucine-rich repeat) and forming active functional polypeptide, and is capable of simply removing two tag members through once resection enzyme cutting, thereby preparing high-purity high-activity LRR sequence.

The current invent relates to the construction and application of a three-line breeding system in the Peruvian scallop×bay scallop hybrids. The said three-line consists of a male sterile line, a maintainer line and a restorer line of the bay scallop×Peruvian scallop hybrids. Herein the Peruvian scallop×bay scallop hybrids are hybrids resulted from the eggs of the Peruvian scallops fertilized with sperm of the bay scallops. In constructing the three-line, male sterile individuals are selected from F1 inter-specific hybrid families and continuously backcrossed with sperm of the selling family of the sperm-providing bay scallops of the F1 hybrid to obtain a combination of male sterile line and maintainer line whose hybrid progenies are all male sterile. Then selected individuals from the male sterile line are continuously backcrossed with sperm from the selfing family of a bay scallop until the resulted progenies are all hermaphroditic and exhibit excellent production traits. Commercial male sterile brood stocks are produced by backcrossing the eggs of the male sterile line with the sperm of the maintainer line and commercial Peruvian scallop×bay scallop hybrid spats are produced by backcrossing the eggs of the male sterile line and sperm of the restorer line. This invent establishes the three-line breeding system in animals for the first time and efficiently overcomes the barrier of self-fertilization in the hybridization between hermaphroditic scallops. The yield of the hybrid scallops can be increased by over 50% compared with the bay scallops.

The invention provides a niobium-iron alloy continuous production device and a production method. The device comprises a smelting furnace, a slag pool and an alloy molten pool; and an alloy generatedin the smelting furnace is molten through siphon separation to discharge from the alloy molten pool, and waste slag is discharged from the slag pool. The device can realize continuous production of aniobium-iron alloy, is higher in heat utilization efficiency in the process, improves the utilization rate of waste heat by above 20%, can further reduce the use level of a smelting furnace material heating agent, realizes clean production of the iron alloy, is low in comprehensive energy consumption, is prominent in energy saving and consumption reducing effects, greatly reduces the production cost, in particular, can realize the reduction operation of production procedures through application of a thermal molten alloy granulation breakage-free process, greatly reduces the slag-rich generation ratio compared with an intermittent production process, improves the procedure metal yield by 2% or above, is excellent in comprehensive economic benefit, improves the production efficiency by above30% compared with a traditional process, is convenient and efficient in production, is environment-friendly, is obvious in technical progress and innovation demonstration effect, improves the productquality, and is prominent in social and economic benefits.

The invention relates to the electric wire and electric cable manufacture field, and particularly to a plastic extruding mould required by production of a concentric sparse winding shielding layer of a plurality of single-wire conductors under a cable sheath layer. Disclosed is the plastic extruding mould for producing electric cables. The plastic extruding mould includes a plastic extruding mould base; a male mould and a female mould are respectively arranged at two sides of a cavity in the plastic extruding mould base, a mould cavity is formed between the male mould and the female mould, the female mould is connected with a forming pipe, and the male mould is connected with a mould body; the interior of the mould body is connected with a stranding cage through bearings, the interior of the stranding cage is provided with a threading pipe, the stranding cage can rotate around the threading pipe, the right end of the stranding cage is provided with a distributing terminal board, and the distributing terminal board is provided with wire harness conduits. A wire core is passed through the threading pipe, then through the male mould and the female mould, next through the forming pipe, and is connected with a traction machine set; and sparse winding single wires are passed through the wire harness conduits and the distributing terminal board.

The invention provides a piston type bean sprouting machine. The piston type bean sprouting machine comprises a frame (1); a cylindrical structure (2) is arranged in the frame; the cylindrical structure (2) is provided with a supporting plate (3); a water catchment basket (4) is arranged under the supporting plate; bean materials are put on the supporting plate; a piston type bearing plate (5) is arranged above the bean material region; in use, the piston type bearing plate (5) is raised in a piston manner by the lifting force generated by the sprouting of the bean materials, and therefore, the production quality of the bean sprout is improved; water catchment holes are formed in the supporting plate (3) and the root system of the bean sprout absorbs moisture from the water catchment basket; a sensor (6) is arranged on the upper portion of the cylindrical structure (2) and used for detecting the production status of the bean sprout and immediately sending out prompt information by use of voice or LED (Light-Emitting Diode) lighting; as the production status of the bean sprout can be detected and the prompt information can be sent out immediately, the yield can be increased.

The invention discloses hand-washing-free disinfection gel convenient and easy to produce and a preparation method of the hand-washing-free disinfection gel, and relates to the field of disinfectant preparation. The hand-washing-free disinfection gel comprises the following components in percentage by weight: 40-89% of ethanol; 10-40% of water; 0.1-10% of glycerol; 0.01-1% of a bactericide; 0.01-5% of a thickening agent; 0.01-10% of a pH regulator; 0.005-1% of a defoaming agent; wherein the thickening agent is a cationic cellulose thickening agent. According to the hand-washing-free disinfection gel, the cationic cellulose thickening agent and the defoaming agent are added, so that the defects of long production period and complex production process caused by long dissolving time of the thickening agent during production of a traditional hand-washing-free disinfection gel product and standing after production are avoided, and efficient and convenient production of the hand-washing-free disinfection gel is realized. As cations in the cationic cellulose thickening agent are uniformly distributed on a main chain of a polymer, the cationic cellulose thickening agent has higher viscosity when being independently dispersed in a water phase, and the production period of producing a hand washing gel product can be greatly shortened.

The invention relates to an optical fiber guiding device suitable for a secondary plastic coating technology. The optical fiber guiding device consists of a floor stand, telescopic rods and guide pulley groups, wherein an upper horizontal telescopic rod and a second telescopic rod are hinged to the floor stand; a first telescopic rod is connected to the front end of the upper horizontal telescopic rod; the two guide pulley groups are respectively mounted at the front end of the first telescopic rod and the front end of the second telescopic rod. The optical fiber guiding device disclosed by the invention is simple in structure and reasonable in design; the telescopic rods and the guide pulley groups are arranged, so that optical fibers in different specifications are guided; the optical fiber guiding device has multiple purposes, is convenient and efficient; the problems that in the prior art, in the mass production process, the guiding of the optical fibers in various specifications is not liable to control, and the work is many and diverse, time-consuming and labor-consuming are solved; the accurate control of the remained length of bundled pipes is facilitated, so that the production is efficient and convenient, and the optical fiber guiding device disclosed by the invention is suitable for wide popularization and application.

Substrate components for packaging IC chips and electronic device packages are disclosed. A substrate component for packaging IC chips comprises: a glass core base with at least one conductive through via connecting a combination of metallization and dielectric structures on both an upper surface and a lower surface of the glass core base; and, tapered edges created at a peripheral region of the glass core base; wherein dielectric layers are disposed over the tapered edges at the peripheral region of the glass core base. In accordance with an embodiment of the invention, the dielectric layers have a substantial planar upper surface, a lower surface conformably interfaced with the tapered edges at peripheral region of the glass core base, and a steep cutting face with the tapered edges of the glass core base. Alternatively, the tapered edges at peripheral region of the glass core base are not covered by the dielectric layers, and an encapsulated material sealing the tapered edges at peripheral region of the glass core base.