47results about How to "Efficient and convenient production" patented technology

The present invention comprises a method for producing libraries of expressible gene sequences. The method of the invention allows for the simultaneous manipulation of multiple gene sequences and thus allows libraries to be created in an efficient and high throughput manner. The expression vectors containing verified gene sequences can be used to transfect cells for the production of recombinant proteins. The invention further comprises libraries of expressible gene sequences produced using the method of the invention and expression vectors used in the construction of said libraries.

The invention discloses fusion protein containing a leucine-rich repetitive sequence, and a preparation method and application thereof. Concretely, the invention relates to fusion protein, and the fusion protein possesses the following structure from N terminal to C terminal: A-X-E-Y1-Y2 (formula Ia) or A-Y2-Y1-E-X (formula Ib), wherein A is an optional secretion signal peptide, X is a member rich in leucine repetitive sequence 1-2 (LRRs1-2) of Slit2 protein (neuronal guidance factor 2), E is a restriction enzyme cutting site, Y1 is a first tag peptide member, Y2 is a second tag short peptide member and the second tag short peptide member is His tag member, and '-' represents a peptide bond or a peptide joint for connecting the above members. The fusion protein is beneficial for correct folding of LRR (leucine-rich repeat) and forming active functional polypeptide, and is capable of simply removing two tag members through once resection enzyme cutting, thereby preparing high-purity high-activity LRR sequence.

Provided is a new production method for the synthesis of an NC type nucleoside efficiently and conveniently in a high yield without unnecessary protecting group conversion steps. It relates to a step of producing a compound represented by the formula (II):

or a salt thereof, by inverting a compound represented by the formula (I):

and a method of producing a compound represented by the formula (III):

or a salt thereof (wherein each symbol is as defined in the specification), which includes the step.

The invention discloses fusion protein containing a leucine-rich repetitive sequence, and a preparation method and application thereof. Concretely, the invention relates to fusion protein, and the fusion protein possesses the following structure from N terminal to C terminal: A-X-E-Y1-Y2 (formula Ia) or A-Y2-Y1-E-X (formula Ib), wherein A is an optional secretion signal peptide, X is a member rich in leucine repetitive sequence 2 (LRRs2) of Slit2 protein (neuronal guidance factor 2), E is a restriction enzyme cutting site, Y1 is a first tag peptide member, Y2 is a second tag short peptide member and the second tag short peptide member is His tag member, and '-' represents a peptide bond or a peptide joint for connecting the above members. The fusion protein is beneficial for correct folding of LRR (leucine-rich repeat) and forming active functional polypeptide, and is capable of simply removing two tag members through once resection enzyme cutting, thereby preparing high-purity high-activity LRR sequence.

The invention relates to the electric wire and electric cable manufacture field, and particularly to a plastic extruding mould required by production of a concentric sparse winding shielding layer of a plurality of single-wire conductors under a cable sheath layer. Disclosed is the plastic extruding mould for producing electric cables. The plastic extruding mould includes a plastic extruding mould base; a male mould and a female mould are respectively arranged at two sides of a cavity in the plastic extruding mould base, a mould cavity is formed between the male mould and the female mould, the female mould is connected with a forming pipe, and the male mould is connected with a mould body; the interior of the mould body is connected with a stranding cage through bearings, the interior of the stranding cage is provided with a threading pipe, the stranding cage can rotate around the threading pipe, the right end of the stranding cage is provided with a distributing terminal board, and the distributing terminal board is provided with wire harness conduits. A wire core is passed through the threading pipe, then through the male mould and the female mould, next through the forming pipe, and is connected with a traction machine set; and sparse winding single wires are passed through the wire harness conduits and the distributing terminal board.

The invention discloses fusion protein containing a leucine-rich repetitive sequence, and a preparation method and application thereof. Concretely, the invention relates to fusion protein, and the fusion protein possesses the following structure from N terminal to C terminal: A-X-E-Y1-Y2 (formula Ia) or A-Y2-Y1-E-X (formula Ib), wherein A is an optional secretion signal peptide, X is a member rich in leucine repetitive sequence 2 (LRRs2) of Slit3 protein (neuronal guidance factor 3), E is a restriction enzyme cutting site, Y1 is a first tag peptide member, Y2 is a second tag short peptide member and the second tag short peptide member is His tag member, and '-' represents a peptide bond or a peptide joint for connecting the above members. The fusion protein is beneficial for correct folding of LRR (leucine-rich repeat) and forming active functional polypeptide, and is capable of simply removing two tag members through once resection enzyme cutting, thereby preparing high-purity high-activity LRR sequence.

The present invention discloses car mat structures used to set on the floor compartment area in a car to contain water drops or debris fallen or brought into the car. One car mat structure includes a bottom pad with plural sidelines; and plural dams, each being raised from one said sideline, wherein two opposite members of the dams are dual slop walls each being built with two inclined surfaces with different inclined angles. Another car mat structure includes a bottom pad; plural dams raised from the sidelines of the bottom pad; and plural triangle shutters formed to cover bottom corners each formed by two adjacent dams and the bottom pad. A third car mat structure includes a bottom pad; plural dams raised from the sidelines of the bottom pad, wherein two opposite members of the dams each being built with two inclined surfaces with different inclined angles; and plural triangle shutters. With the implementation of the new design dams and triangle shutters of the present invention, molding is easier when forming and producing mat structures; cleaning becomes easier since no hidden corners existing on the mat structures; and deformation or collapsing of mat structures being effectively prevented. When further build an inclined cover pad to cover the junction area between a dam and the bottom pad, molding for production and cleaning the mats become even more convenient and efficient.

The gene for Streptococcus pyogenes DNase B has been cloned and vectors incorporating the cloned DNA have been used to transform Escherichia coli, allowing efficient and rapid production of the DNase in E. coli without the necessity of growing large quantities of S. pyogenes. The enzyme can be produced with a leader peptide at its amino-terminus. An improved method for the purification of naturally occurring S. pyogenes DNase B enzyme is also provided. The DNase B enzyme produced, either by purification of naturally occurring enzyme or by recombinant DNA techniques, can be used to generate antibodies and can also be used in immunochemical assays to detect the presence of anti-DNase B antibodies in serum as a marker of infection by S. pyogenes.

The present invention relates to a gene construct which is capable of achieving efficient production of an antimicrobial peptide in a microorganism, and a method for efficient mass production and separation of an antimicrobial peptide using the same. The gene construct of the present invention has a translationally coupled configuration of two independent and separate cistrons which encode an acidic peptide and a basic antimicrobial peptide, each having an opposite charge, under the control of a single promoter. The translationally coupled acidic peptide and basic antimicrobial peptide undergo charge-charge interaction simultaneously with expression thereof to neutralize the potential cytotoxicity of the antimicrobial peptide, resulting in prevention of antimicrobial peptide-mediated killing of host microorganisms. In addition, a conjugate of the acidic peptide and the antimicrobial peptide can be separated without chemical or enzymatic treatment. Therefore, it is possible to achieve easy mass production of antimicrobial peptides from recombinant microorganisms.

The invention provides a method of producing mutant of tumour necrosis factor called TNF-alphaand the related technology about expression carrying agent and, the construction, expression and decontamination of engineering cell. The protein gene of mutant of tumour necrosis factor called TNF-alpha,which has been optimized, is very much suitable for expression in pichia, meanwhile, improves expression quantity and the rate of purification through the optimization of fermentation and decontamination, has the advantages of high expression, good stability, compact production technology. This invention can get the protein gene of mutant of tumour necrosis factor called TNF-alpha with performance, briefness and low cost.

The invention relates to a reconstruction interferon gamma nucleotide sequence and the manufacture method to produce reconstruction interferon gamma, and the relative expression carrier and engineer cell construction, expression and purification technology. The method improves the expression quantity and the purification yield. It has the advantage of high expression, good stability, and simple technology. It could gain reconstruction human interferon gamma high efficiently, conveniently and in low cost.

A rapid beverage cooling device and a refrigeration method thereof are provided. The rapid beverage cooling device includes an outer cup body and an inner cup body of which rims are connected. An interlayer inner cavity for storing a liquid is formed between the outer cup body and the inner cup body; a liquid injection port communicated with the interlayer inner cavity is made at a bottom of the outer cup body; the liquid injection port can be opened or sealed; a liquid accommodating cavity is disposed in the inner cup body; a convex column extends toward the liquid accommodating cavity from a wall of the inner cup body; and an inner cavity of the convex column is communicated with the interlayer inner cavity.

The invention discloses fusion protein containing a leucine-rich repetitive sequence, and a preparation method and application thereof. Concretely, the invention relates to fusion protein, and the fusion protein possesses the following structure from N terminal to C terminal: A-X-E-Y1-Y2 (formula Ia) or A-Y2-Y1-E-X (formula Ib), wherein A is an optional secretion signal peptide, X is a member rich in leucine repetitive sequence 1-2 (LRRs1-2) of Slit3 protein (neuronal guidance factor 3), E is a restriction enzyme cutting site, Y1 is a first tag peptide member, Y2 is a second tag short peptide member and the second tag short peptide member is His tag member, and '-' represents a peptide bond or a peptide joint for connecting the above members. The fusion protein is beneficial for correct folding of LRR (leucine-rich repeat) and forming active functional polypeptide, and is capable of simply removing two tag members through once resection enzyme cutting, thereby preparing high-purity high-activity LRR sequence.

The current invent relates to the construction and application of a three-line breeding system in the Peruvian scallop×bay scallop hybrids. The said three-line consists of a male sterile line, a maintainer line and a restorer line of the bay scallop×Peruvian scallop hybrids. Herein the Peruvian scallop×bay scallop hybrids are hybrids resulted from the eggs of the Peruvian scallops fertilized with sperm of the bay scallops. In constructing the three-line, male sterile individuals are selected from F₁ inter-specific hybrid families and continuously backcrossed with sperm of the selling family of the sperm-providing bay scallops of the F₁ hybrid to obtain a combination of male sterile line and maintainer line whose hybrid progenies are all male sterile. Then selected individuals from the male sterile line are continuously backcrossed with sperm from the selfing family of a bay scallop until the resulted progenies are all hermaphroditic and exhibit excellent production traits. Commercial male sterile brood stocks are produced by backcrossing the eggs of the male sterile line with the sperm of the maintainer line and commercial Peruvian scallop×bay scallop hybrid spats are produced by backcrossing the eggs of the male sterile line and sperm of the restorer line. This invent establishes the three-line breeding system in animals for the first time and efficiently overcomes the barrier of self-fertilization in the hybridization between hermaphroditic scallops. The yield of the hybrid scallops can be increased by over 50% compared with the bay scallops.

The invention provides a piston type bean sprouting machine. The piston type bean sprouting machine comprises a frame (1); a cylindrical structure (2) is arranged in the frame; the cylindrical structure (2) is provided with a supporting plate (3); a water catchment basket (4) is arranged under the supporting plate; bean materials are put on the supporting plate; a piston type bearing plate (5) is arranged above the bean material region; in use, the piston type bearing plate (5) is raised in a piston manner by the lifting force generated by the sprouting of the bean materials, and therefore, the production quality of the bean sprout is improved; water catchment holes are formed in the supporting plate (3) and the root system of the bean sprout absorbs moisture from the water catchment basket; a sensor (6) is arranged on the upper portion of the cylindrical structure (2) and used for detecting the production status of the bean sprout and immediately sending out prompt information by use of voice or LED (Light-Emitting Diode) lighting; as the production status of the bean sprout can be detected and the prompt information can be sent out immediately, the yield can be increased.