The invention belongs to the technical field of chromatographic detection, and specifically relates to a method for quickly detecting
vancomycin by utilizing LC-MS (Liquid
Chromatography-
Mass Spectrometry). The method comprises the following steps: smashing a to-be-detected sample, adding an extracting
reagent, and uniformly mixing; then carrying out ultrasonic extraction, and centrifuging, thus obtaining supernatant; extracting the supernatant by adopting water saturated normal
hexane, thus obtaining a to-be-purified solution; activating through an MCX
solid-phase extraction column, taking and sampling the to-be-purified solution, carrying out drip washing and
impurity removing by using
methanol, then eluting by using amonium
methanol, and collecting an eluent; concentrating, forming a constant volume by using a constant volume solution, and filtering, thus obtaining a purified solution; detecting the purified solution by adopting an HPLC-MS (High Performance Liquid Chromatograph-MassSpectrometer). The method disclosed by the invention has the advantages that the problems of large matrix effects, low sensitivity and the like existing in a process of detecting animal derived foodand particularly the animal derived food with a
high fat content in the prior art are well solved, ideal
recovery and precision degree are obtained, the minimum
detection limit of the method is 0.3 mug / kg, and
repeatability and reproducibility of an analysis result are effectively ensured.