The invention belongs to the technical field of coatings, and specifically relates to a graphene-silver antibacterial and antifouling fluorocarbon coating, and a preparation method and an applicationthereof. The graphene-silver antibacterial and antifouling fluorocarbon coating comprises a component A and a component B, wherein the component A comprises the following components: fluorocarbon resin, a wetting dispersant, a leveling agent, an antifoaming agent, a thixotropic agent, a graphene-silver composite nanometer material, titanium dioxide, precipitated barium sulfate, quartz powder, butyl acetate and propylene glycol methyl ether acetate; and the component B comprises an isocyanate curing agent. According to the invention, due to introduction of the graphene-silver composite nanometer material, a paint film has good adhesion force, impact resistance, hydrophobicity, stable broad-spectrum antibacterial property and antifouling property; in addition, through selection of the fluorocarbon resin with a fluorine content of no less than 26%, glossiness, aging resistance and mechanical properties of the paint film are improved; meanwhile, low surface energy of the paint film is guaranteed; and the paint film prepared from the coating provided by the invention has strong hydrophobicity.