The invention provides an intelligent regulation and control system of a clean room. The system comprises an operation station, a communication interface controller, an intelligent control device, clean room operation equipment and clear room parameter collection equipment, wherein both of the clear room parameter collection equipment and the clean room operation equipment are in signal connection with the intelligent control device, and the intelligent control device is connected to the operation station through the communication interface controller. According to the intelligent regulation and control system of the clean room provided by the invention, the operation station can master the working state of the clean room operation equipment, realize remote start and stop and adjust the parameters of the clean room, thereby greatly perfecting and improving the system management level, manual layer-by-layer start, control, management and regulation of the clean room operation equipment are converted into automation, informatization, networking and intelligence, and the intelligent regulation and control system integrates remote automatic control, real-time monitoring, energy saving, consumption reduction and other human-based management service functions.