The invention provides a preparation method of a dissolved oxygen sensing membrane head and the dissolved oxygen sensing membrane head, and aims to solve the problems of long response time and easy falling of a fluorescent layer in the sensing membrane head in the prior art. The preparation method comprises the following steps: dissolving an indicator in a tetrahydrofuran solution to obtain an indicator solution, and adding the indicator solution into an emulsion of polystyrene microspheres while stirring; adding a calcium chloride aqueous solution, stirring, carrying out ultrasonic treatment, pouring onto a quartz plate, volatilizing, drying and curing to obtain a fluorescent layer; adding acrylic resin, styrene, ethyl acetate, xylene and organic silicone into a test tube, stirring and dissolving, spraying on the fluorescent layer, and drying to obtain a reflecting layer; dichloromethane, butyl acetate, polyacrylic resin, polystyrene, carbon black, paraformaldehyde, sulfonamide formaldehyde resin, simethicone, titanium dioxide powder, polyvinyl chloride, ethyl cellulose and stearic acid are mixed, stirred and dissolved, then sprayed to the reflecting layer and dried, and the shading layer is obtained. The fluorescent layer is not easy to elute.