The invention provides a preparation method of a deuterium-depleted water beverage for preventing and treating 'four highs'. The method comprises the following steps: 1) preparing lentinan; 2) preparing soy protein peptide hydrolyzate; 3) placing hawthorn, straight ladybell root, platycodon, mulberry leaf, pueraria, purslane, medlar, corn stigma, astragalus and chicory in deuterium-depleted water,boiling for 2-3 hours to obtain a Chinese herbal extract; 4) mixing the lentinan and the soy protein peptide hydrolyzate with the Chinese herbal extract obtained in the step 3) and conducting sterilization to obtain the deuterium-depleted water beverage for preventing and treating 'four highs'. Compared with the prior art, the deuterium-depleted water is adopted as one of the main raw materials,the preparation process of the lentinan and the soy protein peptide is controlled, the dosage of the hawthorn, the straight ladybell root, the platycodon, the mulberry leaf, the pueraria, the purslane, the medlar, the corn stigma, the astragalus and the chicory is designed, and the effects of lowering the 'four highs' of high blood fat, high blood sugar level, high blood pressure and high uric acid can be achieved the synergistic effect of the various raw materials.