The invention discloses an optical fiber guide fixator used in the communication field, and relates to the technical field of optical fiber fixation. The optical fiber guide fixator comprises a fixingblock and a guide block, the fixing block is provided with a sliding groove, and the guide block is slidably connected in the sliding groove. The bottom of the sliding groove is provided with a firstthrough groove, and the side, close to the fixing block, of the guide block is provided with a second through groove. An arc-shaped ring groove is formed at one side, deviating from the fixed block,of the guide block, one side of the arc-shaped annular groove is communicated with the second through groove, and the other side penetrates through the guide block to form an opening on the guide block. A rotating ball is arranged in the arc-shaped annular groove and abuts against the opening, a third through groove is formed at the side, close to the opening, of the rotating ball, the diameter ofthe third through groove is smaller than that of the opening, and the third through groove is communicated with the second through groove. The optical fibers are arranged in order according to the number or groups, and the rotating ball is adjusted for fixing according to the different connection extension directions of the optical fibers, thereby improving the beauty of the interface end of a device or the outlet end of a terminal box, and facilitating the operation of a worker during the later inspection and maintenance.