The invention belongs to the technical field of specimen transfer, particularly relates to a microbial specimen transfer device, and provides the following scheme for solving the problem of poor storage environment: the microbial specimen transfer device comprises an equipment box and a transfer box, a placement plate is horizontally and slidably connected in the transfer box, and a placement groove is formed in the surface of the placement plate; the equipment box comprises a draught fan chamber, a heating chamber, a condensation chamber, a backflow chamber and a water storage chamber, a heating pipe is vertically installed in the heating chamber, a condenser is installed at the bottom in the condensation chamber, a water pump is installed on one side of the bottom in the water storage chamber, and a temperature sensor and a humidity sensor are installed in the middle of the top in the transfer box side by side. A bottom plate is arranged below the transfer box, and a damping structure is arranged between the transfer box and the bottom plate. The optimal temperature and humidity of microorganism survival are kept, the survival state of microorganisms in the transfer process is guaranteed, shock absorption can be conducted on the transfer box, the shock absorption effect is good, and test tubes or cultured mediums are prevented from vibrating and being damaged.