The invention discloses a flexible OLED substrate. The flexible OLED substrate comprises a flexible transparent substrate, a transparent conductive layer, a water and oxygen blocking layer and an electrode modification layer, wherein the flexible transparent substrate and the transparent conductive layer are arranged opposite to each other, the water and oxygen blocking layer is arranged between the flexible transparent substrate and the transparent conductive layer, the electrode modification layer is arranged at one side, deviating from the water and oxygen blocking layer, of the transparentconductive layer, the sheet resistance of the transparent conductive layer is 10 ohms per sheet, the light transmittance is larger than 85%, and the water and oxygen permeation rate is lower than 10<-6>g/m<2>/d. The water and oxygen blocking layer having relatively high water and oxygen blocking performance is arranged, the transparent conductive layer with high conductivity, high light transmittance and low surface roughness is arranged, thus, the uniformity of an electric field in the device is effectively improved, the internal resistance energy consumption is reduced, and the performanceof the device is optimized; and moreover, a conductive polymer is also introduced as the electrode modification layer, and the smoothness of a surface of the transparent conductive layer is further improved. The flexible OLED substrate can be used for a flexible OLED display and lighting device and has the advantages of low internal consumption, good light-emitting uniformity, high winding resistance and long lifetime.