The present invention discloses a method for labeling high-concentration
colloidal gold. Buffer solutions of different pH values are taken to dilute high-concentration
colloidal gold solutions respectively to form
colloidal gold solutions of different pH values. Proteins to be labeled are added to each colloidal gold solution respectively, and then the optimal labeling pH is determined by a high-salt destruction test. A labeling
buffer solution of the optimal labeling pH is taken to dilute the high-concentration colloidal gold solution, and then the solution is divided into several equal parts, and
protein solutions to be labeled are added respectively to form a
mixed solution of markers with different final
protein concentrations, and then the optimal
protein labeling amount is determined by a high-salt destruction test. A
mixed solution of markers is prepared according to the conditions for determining the optimal labeling and the pH optimal
protein labeling amount, and then a blocking reaction is performed to obtain a colloidal gold labeled protein complex. In the labeling process of the present invention,
gold particles do not aggregate throughout the entire process, the
reaction system has a small volume, 60% of labor is saved,
protein materials are saved, and in centrifugal purification, large-scale production can be completed without investing too much large-scale equipment.