The invention belongs to the field of biomedical engineering, and relates to a medical multifunctional protective clothing material and a preparation method thereof. The material comprises a protective layer, an isolation layer and a protective layer which are sequentially arranged, the lower surface of the protective layer is connected with the upper surface of the isolation layer, the lower surface of the isolation layer is connected with the upper surface of the protective layer, the protective layer, the isolation layer and the protective layer are compounded into a whole, the protective layer is made of polyimide fiber fabric, the isolation layer is made of multifunctional nano-microporous film materials, and the protective layer is a polyimide fiber blended fabric. By utilizing hydrolysis resistance, high-temperature resistance, flame retardance, acid and alkali resistance, radiation resistance, oxidation resistance, antibacterial property, skin friendliness, good processing performance, good air and moisture permeability, high-temperature resistance and high-pressure resistance of the polyimide fibers, the polyimide fibers can be repeatedly used after being cleaned and disinfected, and the problems that disposable protective clothing is poor in air and moisture permeability and cannot be repeatedly used are solved.