31results about How to "Signal strength is stable" patented technology

A mass spectrometer is disclosed comprising a separation device arranged and adapted to emit an eluent over a period of time. The separation device preferably comprises a Capillary Electrophoresis (“CE”) separation device. The mass spectrometer further comprises a nebuliser and a target. Eluent emitted by the separation device is nebulised, in use, by the nebuliser wherein a stream of analyte droplets are directed to impact upon the target so as to ionise the analyte to form a plurality of analyte ions.

The invention discloses a positioning method which comprises the following steps: receiving a positioning request transmitted by a mobile node, wherein the positioning request contains signal intensity time sequence data of the mobile node, and the signal intensity time sequence data is data for representing the change of wireless signal intensity along with time when the mobile node moves; inquiring signal intensity time sequence target data matched with the data of the mobile node in a pre-established signal intensity time sequence map, wherein the signal intensity time sequence map is used for recording the signal intensity time sequence data of the detected mobile node on different road sections; determining positioning data corresponding to the target data as positioning data of the mobile node. According to the positioning method provided by the invention, on the premise of ensuring the mobile positioning accuracy, the energy consumption required for positioning can be reduced. The invention further discloses a positioning device and a system.

The invention is based on the use of photoluminescent probes for intracellular sensing of oxygen, especially assaying intracellular oxygen concentration. The photoluminescent probe comprises a suspension of polymeric particles having an average diameter in the 20 nm to 100 nm range, formed from an amphiphilic cationic co-polymer which is oriented in the formed particle to provide a hydrophobic core and a hydrophilic shell. The probe includes a hydrophobic oxygen-sensitive photoluminescent dye such as Pt-tetrakis(pentafluorophenyl)porphine, PtPFPP embedded in the hydrophobic core of the particle, and the co-polymer includes quaternary ammonium groups which provide hydrophilic and cationic character to the particle shell. The photoluminescent probe, which in use is provided in the form of an aqueous suspension of probe, is incubated with live mammalian cells in a suitable growth medium for a period of time to allow the probe particles passively load into the cells. Oxygen can then be sensed by detecting a photoluminescent signal of the photoluminescent probe, which can be correlated with oxygen status, for example oxygen concentration or changes in oxygen concentration/levels, using existing techniques.

A high-pressure formed substrate film assisted laser desorption dissociation mass spectrum quantitative analysis method is disclosed. According to the method, an organic micro-molecular substrate is compacted under high pressure to obtain a film; the film is then adhered to a sample target of a mass spectrometer; a sample is pointed on the film to be naturally dried; the sample undergoes in situ cleaning to remove inorganic salt; and after natural drying, the sample is putted into the mass spectrometer for analysis. By the adoption of the method, size and shape inhomogeneity of crystals obtained by a crystallization process is avoided. The obtained substrate film can have an effect of a hydrophobic reverse-phase stationary phase and adsorbs sample molecules. Inorganic salt is removed by in situ cleaning, and the sample molecules are prevented from spreading on the surface of the sample target so as to raise sensitivity. The method provided by the invention has advantages of stable mass spectrum signal, good reappearance, little metal ion adduct interference, small spectra background, good linear relation between signal intensity and sample amount and feasible quantitative analysis. The method is simple, requires no complex apparatus, complex sample pretreatment or organic solvent, and is green and environmentally friendly.

The invention belongs to the technical field of biology, and relates to a multiplex amplification system for rapidly mutating Y-chromosome short tandem repeats, a kit and an application. The multiplexamplification system comprises 16 pairs of primers, and can be used for amplifying 16 STR loci: DYS630, DYS464, DYF403S1b, DYF399S1, DYS518, DYF403S1a, DYS527, DYS713, DYS612, DYS626, DYS627, DYS526,DYF404S1, DYF387S1, DYS449, and DYS547. Compared with prior art, the system is advantageous in that: 1. the system contains 16 human male Y-chromosome high-mutation rate STR, and the system is uniqueand novel with a large amount of information content and good compatibility; 2. the system has wide application range, good directivity, and high precision, and the system is suitable for legal medical expert DNA analysis in material evidence cases which relate to all cells of male; wherein biological testing materials which contain mal cells, such as bloodstain, seminal stain, saliva, hair, nail, cartilage and other human tissues, can be identified. 3. the system has good system specificity and good stability, multiple repeat checking is not needed, non-specific amplification products are not generated, and signal intensity is stable; 4. the system has high sensitivity, and DNA template amount which is low to 15pg can be accurately classified.

The invention discloses a fluorescent composite amplification system, a kit and application of the kit. The fluorescent composite amplification system comprises multiple pairs of specific primers, hasa nucleotide sequence shown in SEQ ID NO.1-71 and is used for preparing the kit, and the prepared kit is applied to DNA analysis and source analysis by legal medical experts. The Y-SNP contains multiple haplogroups and has high discrimination for male groups, and the kit is unique, novel, large in information amount and good in compatibility.

The invention discloses a multispectral ultraviolet sensitivity detection system and a detection method. The detection system comprises a light-emitting device, an attenuation device, a detection device and a verification processing system. The requirement for the experiment environment is low. Ultraviolet test signals with uniform and stable signal intensity can be generated, the signal intensitycan be accurately adjusted. The measurement result can be metered and traced. According to the invention, on one hand, sensitivity detection can be carried out on a single device, so as to evaluate the ultraviolet sensitivity performance of the device; further, sensitivity detection can be carried out on different devices to calibrate and unify the photon number, which promotes the standardization of ultraviolet detection; besides, the spectral analysis function of the system can detect ultraviolet sensitivity of different wavebands to obtain the sensitivity spectral curve of a device; the detection performance is more comprehensive; and the detection requirements of sensitivity of all ultraviolet imagers on the market are met.

The invention discloses a method for improving the output audio signal-to-noise ratio of a sound amplification system, and the method comprises the steps: receiving audio analog signals of a first path and a second path of a sound pick-up, carrying out the high-gain amplification of the audio analog signals of the first path, and carrying out the low-gain amplification of the audio analog signalsof the second path; performing analog/digital conversion to obtain a corresponding first channel audio digital signal and a corresponding second channel audio digital signal; and judging whether the voltage of the first channel audio digital signal is greater than or equal to a preset first voltage threshold, if so, transmitting the second channel audio digital signal as audio output to a sound expander, and otherwise, transmitting the first channel audio digital signal as audio output to the sound expander. The invention also provides an intelligent sound amplification system. According to the invention, the received source audio signal can be gained to obtain the final audio signal with high signal-to-noise ratio, low total harmonic distortion and stable signal strength.

The invention relates to an automatic identification method and device for in-row weeds in the seedling stage of transplanted crops. The device comprises a darkroom system constructed by sectional materials; an RGB camera is fixed at the top end in the darkroom system and is connected with a computer; six color code sensors are fixed at the bottom end in the darkroom system, signal lines of the color code sensors are connected with a computer through an Arduino single chip microcomputer, and power lines of the color code sensors are connected with a transformer; transplanted crop seedlings are located at the symmetric center of the six sensors and right face the RGB camera, and weeds exist on the left side and the right side of the transplanted crop seedlings. Through a series of designs, the signal transduction technology is adopted, images captured from the top are analyzed, transplanted crop seedlings and weeds in the images are selected in a frame mode, and then the transplanted crop seedlings and weeds are distinguished through information sent back by a color code sensor. A new machine vision and signal transduction method based on the geometric appearance of the transplanted crop seedlings is established, and the positions of the crop seedlings and weeds in the field are accurately recognized.

The invention relates to an exosome preparation method and application. The method comprises at least the following steps: a culture supernatant of an organoid is processed via a differential centrifugation method, and an exosome is obtained after separation. According to the preparation method, the exosome can be separated and purified in a high-efficiency way; the exosome of the organoid can reserve in-situ tissue characteristics and can provide intercellular, histiocytic and extracellular matrix information; and the exosome of the organoid can provide better targeted and more comprehensivein-situ tissue features and is beneficial to the research on inter-cancer-cellular matrix and cancer cellular and cytoplasmic matrix and to the advancement of cancer treatment.