The invention relates to a preparation method of embedded double-layer bletilla striata dressing, and belongs to the technical field of biological medicines. The preparation method comprises the following steps: dissolving bletilla striata polysaccharide extracted from bletilla striata tuber in water, thus preparing a casting solution, taking part of the bletilla striata polysaccharide casting solution, carrying out treatment by adopting sodium hydroxide, thus obtaining an embedded-layer casting solution, carrying out directional freezing on the bletilla striata polysaccharide casting solutionfor film forming, carrying out groove carving on the surface of the formed film, pouring the embedded-layer casting solution on the formed film with groove carving under liquid nitrogen refrigeration, thus a double-layer formed film is obtained through refrigeration, and carrying out freeze drying on the double-layer formed film, thus obtaining the embedded double-layer bletilla striata dressing.According to the preparation method provided by the invention, the double-layer bletilla striata dressing is prepared by adopting groove carving and freezing, the combination is reliable, the processis simple, and the problems that during taking for use, the bletilla striata dressing is easily soluble, can adhere to gloves, and has difficulty in separating from the applied wound are solved. Theobtained bletilla striata dressing can be degraded and absorbed in the body, has good compatibility with the living body, and has no side effects to the wound, the directional pore structure guarantees the efficient absorption of the dressing for the penetrating fluid, and meanwhile, the dressing is soft and can not cause injuries to the wound.