The invention discloses a guiding type live fish classification device. The guiding type live fish classification device comprises a fish inlet hopper, one or more left fish guiding plates, one or more right fish guiding plates, two adjusting knobs, a fish collecting tank, a fish outlet, a plurality of scraping rods and a support, wherein fishes enter the guiding type live fish classification device through the fish inlet hopper, the left fish guiding plates and the right fish guiding plates are arranged in an opposite and spaced mode, and are used for guiding the inlet fishes, the fish inlet hopper is communicated with one side of each left fish guiding plate and one side of the corresponding right fish guiding plate, the two adjusting knobs are perpendicularly connected with the left fish guiding plates and the right fish guiding plates respectively through two pull rods so that each pull rod can be adjusted to drive the gap between the corresponding left fish guiding plate and the corresponding right fish guiding plate and enable the size of the gap to be increased or decreased, the fish collecting tank is used for collecting live fishes which fall off through the gap between each left fish guiding plate and the corresponding right fish guiding plate, and is arranged below the left fish guiding plates and the right fish guiding plates, the fish outlet is communicated with the fish collecting tank, the scraping rods are evenly arranged below the left fish guiding plates and the right fish guiding plates and are perpendicular to the left fish guiding plates and the right fish guiding plates, the scraping rods are arranged on a chain which is constrained by a tensioning chain wheel and a driven chain wheel, the tensioning chain wheel is connected with a driving motor, and the support is used for supporting the left fish guiding plates, the right fish guiding plates, the tensioning chain wheel, the driven chain wheel, the fish outlet and the driving motor. The guiding type live fish classification device has the advantages of being simple in structure, convenient to use, capable of rapidly and accurately classifying the live fishes and the like.