The invention provides a piezoelectric sensor pre-tightening device, belongs to the field of sensors and measuring and control devices of the sensors and particularly relates to application of a piezoelectric quartz sensor in the field of three-dimensional force measurement. The piezoelectric sensor pre-tightening device consists of a stress block, an upper three-dimensional piezoelectric sensor, a lower three-dimensional piezoelectric sensor, a stud, a nut, an upper press block, a lower press block and a plurality of T-shaped groove nuts. The piezoelectric sensor pre-tightening device eliminates interference brought by pre-tightening force change by using the two sensors, avoids direct contact between an elastic pre-tightening part and the stress block, accordingly eliminates the influence on outputting, of the pre-tightening force change, when the pre-tightening part is stressed to deform, and improves consistency and accuracy of area measurement. The piezoelectric sensor pre-tightening device can perform three-dimensional force measurement, and a reliable measurement area can be further expanded through combined usage. The piezoelectric sensor pre-tightening device is simple in structure, easy to adjust, flexible and convenient to use and wide in application.