The invention belongs to the technical field of polyurethane coatings, and discloses an organic silicon modified hydroxyl polyacrylate dispersion as well as a preparation method and application thereof. The preparation method comprises the following steps: S1, mixing an acrylic monomer, an acrylate monomer, a vinyl monomer, an acrylate derivative monomer, an organic silicon monomer and an initiator to form a mixed solution A; s2, taking a reactive diluent, heating, dropwise adding the mixed solution A, and keeping the temperature; cooling, adding an isocyanate bridging agent, controlling the mass content of-NCO, and reacting to obtain a prepolymer; s3, adding a neutralizer into the prepolymer, adding water for dispersion, and filtering to obtain the product, the organic silicon monomer contains double bonds and does not contain epoxy groups. The organic silicon modified hydroxyl polyacrylate dispersoid prepared by the preparation method disclosed by the invention is low in small molecule alcohol content, high in storage stability and particularly excellent in high-temperature-resistant storage property; and a paint film prepared by using the paint is high in drying speed and high in hardness.