The invention discloses a micro-fluidic chip for viscosity testing, and a preparation method. The chip comprises five structures: a PDMS substrate with a miniature groove, detection electrodes, a PVDF nanofiber film, a PDMS film and a PDMS cover plate with a miniature channel. The preparation method comprises the steps: firstly making the substrate with the miniature groove and the PDMS miniature flow channel cover plate through the photoetching of SU8 photoresist and the demoulding technology; secondly making a pair of detection electrodes at two sides of the miniature groove of the PDMS substrate through employing a mask magnetron sputtering method; thirdly transferring the PVDF film, made through employing an electrostatic spinning method, to the electrodes; finally carrying out the bonding of the substrate and the cover plate, and forming a complete micro-fluidic chip. The micro-fluidic chip is stable in structure, facilitates the integration, is small in number of demands for samples, has no requirements for class, is short in testing time, does not need power, and facilitates the extension of a manufactured micro-fluidic chip viscometer to miniaturization even a hand-held type.