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32results about How to "Increase the degree of lysis" patented technology

Preparation method of carrageenan oligosaccharides

The invention relates to a preparation method of carrageenan oligosaccharides. The preparation method sequentially comprises a raw material pretreatment process step, an extraction process step, an enzymolysis process step, a potassium chloride dissolution process step, a potassic alteration and dehydration process step, a squeezing process step, a sterilization process step, a crushing process step and a material mixing and re-sterilization process step, wherein an irradiation and degradation process step is further contained between the crushing process step and the material mixing and re-sterilization process step, and the irradiation and degradation process step is as follows: a semi-finished product obtained in the crushing process step is simply bagged and packaged, and then irradiated by an electronic accelerator with 10MeV and beam power of 500-650kW for 10-20h. A complex enzyme adopted by the invention has stronger destructive power against cellulose and high degree of carrageenan cracking, and can reduce the using quantity of steam and chemical raw materials in the production process of products and realize energy conservation, consumption reduction and clean production under the situation of ensuring the yield of the products. According to the preparation method provided by the invention, the irradiation method is adopted for degrading large molecular carrageenan into small molecular carrageenan, the addition of any impurities or harmful substances is avoided, and the degradation is fast and pollution-free.
Owner:青岛聚大洋藻业集团有限公司

Method for simultaneously extracting total DNA and RNA from compost

The invention discloses a method for simultaneously extracting total DNA and RNA from compost, which comprises the following steps of: adding corrosion removal buffer solution into a compost sample, shaking and uniformly mixing the solution and the compost, standing the mixture, and centrifuging the suspension; repeating the operation till the centrifuged supernatant is clarified; grinding the sample after corrosion removal by using liquid nitrogen till the sample is powdery, then placing the powdery sample in cracking solution, shaking and uniformly mixing the sample and the solution, putting the mixture in an ice bath, and centrifuging the mixture; taking the cracked supernatant, adding sodium acetate and extraction buffer solution into the supernatant, and centrifuging the mixture after shaking; adding chloroform-isoamylol into the extracted upper aqueous phase, and centrifuging the mixture after shaking; adding isopropanol into the mixture to settle RNA, and washing, drying and dissolving the settled RNA to obtain purified RNA; and adding Tris alkali solution into the extracted organic phase, adding chloroform-isoamylol into the centrifuged upper aqueous phase, centrifuging the mixture, then adding sodium acetate and anhydrous ethanol into the mixture to settle DNA, and washing and dissolving the mixture to obtain purified DNA. The method has the advantages of large yield, good quality, low cost and the like.
Owner:HUNAN UNIV

Auxiliary agent for biomass treatment and biomass gasification treatment method

The invention discloses an auxiliary agent for biomass treatment and a biomass gasification treatment method. The auxiliary agent comprises a component A, a component B and a component C, wherein thecomponent A comprises Lewis acid and one or more of optional organic acid and protonic acid, the component B is a salt of group IA, IIA and IIIA metals and ammonium, and the component C is one or moreof manganese acetate, manganese sulfate and manganese nitrate. The gasification treatment method comprises the following steps: mixing a biomass raw material and an auxiliary agent, and carrying outa reaction to obtain a volatile component and biological semicoke; performing a reaction on the solid-phase materials obtained after gas-solid separation of volatile components and the biological semicoke respectively in a biological coke microwave gasification reactor to obtain crude synthesis gas and activated carbon; and feeding the synthesis gas obtained after gas-solid separation of the crudesynthesis gas into a purification reactor to be treated, thus preparing purified synthesis gas. According to the biomass gasification treatment method and system, the yield of the synthesis gas can be increased, and the tar content in the obtained synthesis gas product is low.
Owner:CHINA PETROLEUM & CHEM CORP +1

A kind of preparation method of carrageenan oligosaccharide

The invention relates to a preparation method of carrageenan oligosaccharides. The preparation method sequentially comprises a raw material pretreatment process step, an extraction process step, an enzymolysis process step, a potassium chloride dissolution process step, a potassic alteration and dehydration process step, a squeezing process step, a sterilization process step, a crushing process step and a material mixing and re-sterilization process step, wherein an irradiation and degradation process step is further contained between the crushing process step and the material mixing and re-sterilization process step, and the irradiation and degradation process step is as follows: a semi-finished product obtained in the crushing process step is simply bagged and packaged, and then irradiated by an electronic accelerator with 10MeV and beam power of 500-650kW for 10-20h. A complex enzyme adopted by the invention has stronger destructive power against cellulose and high degree of carrageenan cracking, and can reduce the using quantity of steam and chemical raw materials in the production process of products and realize energy conservation, consumption reduction and clean production under the situation of ensuring the yield of the products. According to the preparation method provided by the invention, the irradiation method is adopted for degrading large molecular carrageenan into small molecular carrageenan, the addition of any impurities or harmful substances is avoided, and the degradation is fast and pollution-free.
Owner:青岛聚大洋藻业集团有限公司

A kind of auxiliary agent for biomass treatment and biomass gasification treatment method

The invention discloses an auxiliary agent for biomass treatment and a biomass gasification treatment method. The auxiliary agent includes component A, component B and component C; wherein, the component A includes Lewis acid, and any One or more of selected organic acids and protonic acids, the component B is a salt of IA, IIA, IIIA group metal and ammonium, and the component C is one of manganese acetate, manganese sulfate, manganese nitrate species or several. The gasification treatment method is to react the biomass raw material and the auxiliary agent after mixing, and obtain the volatile components and bio-semi-coke after the reaction; the solid-phase materials and bio-semi-coke obtained after the gas-solid separation of the volatile components are respectively fed into the After the biocoke microwave gasification reactor reacts, crude synthesis gas and activated carbon are obtained; the synthesis gas obtained after the gas-solid separation of the crude synthesis gas enters the purification reactor for treatment to obtain purified synthesis gas. The biomass gasification treatment method and system can increase the synthesis gas yield, and the tar content in the obtained synthesis gas product is low.
Owner:CHINA PETROLEUM & CHEM CORP +1

Method for simultaneously extracting total DNA and RNA from compost

The invention discloses a method for simultaneously extracting total DNA and RNA from compost, which comprises the following steps of: adding corrosion removal buffer solution into a compost sample, shaking and uniformly mixing the solution and the compost, standing the mixture, and centrifuging the suspension; repeating the operation till the centrifuged supernatant is clarified; grinding the sample after corrosion removal by using liquid nitrogen till the sample is powdery, then placing the powdery sample in cracking solution, shaking and uniformly mixing the sample and the solution, putting the mixture in an ice bath, and centrifuging the mixture; taking the cracked supernatant, adding sodium acetate and extraction buffer solution into the supernatant, and centrifuging the mixture after shaking; adding chloroform-isoamylol into the extracted upper aqueous phase, and centrifuging the mixture after shaking; adding isopropanol into the mixture to settle RNA, and washing, drying and dissolving the settled RNA to obtain purified RNA; and adding Tris alkali solution into the extracted organic phase, adding chloroform-isoamylol into the centrifuged upper aqueous phase, centrifuging the mixture, then adding sodium acetate and anhydrous ethanol into the mixture to settle DNA, and washing and dissolving the mixture to obtain purified DNA. The method has the advantages of large yield,good quality, low cost and the like.
Owner:HUNAN UNIV
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