The invention discloses a method for simultaneously extracting total
DNA and
RNA from
compost, which comprises the following steps of: adding
corrosion removal
buffer solution into a
compost sample, shaking and uniformly mixing the solution and the
compost, standing the mixture, and centrifuging the suspension; repeating the operation till the centrifuged supernatant is clarified;
grinding the sample after
corrosion removal by using
liquid nitrogen till the sample is powdery, then placing the powdery sample in
cracking solution, shaking and uniformly mixing the sample and the solution, putting the mixture in an ice bath, and centrifuging the mixture; taking the cracked supernatant, adding
sodium acetate and extraction
buffer solution into the supernatant, and centrifuging the mixture after shaking; adding
chloroform-isoamylol into the extracted upper aqueous phase, and centrifuging the mixture after shaking; adding isopropanol into the mixture to settle
RNA, and washing,
drying and dissolving the settled
RNA to obtain purified RNA; and adding
Tris alkali solution into the extracted organic phase, adding
chloroform-isoamylol into the centrifuged upper aqueous phase, centrifuging the mixture, then adding
sodium acetate and
anhydrous ethanol into the mixture to settle
DNA, and washing and dissolving the mixture to obtain purified
DNA. The method has the advantages of large yield, good quality, low cost and the like.