34results about How to "Lower LDL-C levels" patented technology

The invention belongs to the field of antibodies and particularly relates to an anti-human PCSK9 monoclonal antibody and application of the antibody in preparation of drugs for reducing lipoprotein level in blood and preventing or treating cardiovascular diseases or disorders and thrombosis-obstructive diseases or disorders. The anti-human PCSK9 monoclonal antibody has higher affinity and a good application prospect.

The invention discloses a preparing method of seed-melon full powder. The preparing method comprises the steps that seeds and peel of a seed melon are removed; ascorbic acid solutions or citric acid solutions or mixed solutions of ascorbic acid and citric acid are heated to 90-100 DEG C, melon slices are placed in the heated mixed solutions for color protection and precooking for 3-5 min; after cooling, the seed-melon puree is obtained through pulping; an abrasive disk gap is adjusted to be 20-50 microns for glue abrading; 3%-10% of isomaltooligosacharide, 0.05%-0.2% of citric acid, 30%-60% of maltodextrin of soluble solid in material liquid and 3%-10% of honey are added into the solutions to be stirred; mixed puree liquid is pressed by 25-40 Mpa for homogeneity; pasteurization is adopted, and when the pasteurization time is 10-15 min, the pasteurization temperature is 60-70 DEG C; a spray dryer is adopted for drying, and the condition is that the air inlet temperature is 170-180 DEG C, the exhaust air temperature is 78-87 DEG C, and the water content of dried materials is equal to or lower than 5%, and therefore the seed-melon full powder is prepared.

A tea bag with a blood fat reducing action is formed by mixing, smashing and packaging green tea, ampelopsis grossedentata leaves, golden camellia and three-nerved spicebush leaves. The ampelopsis grossedentata leaves, golden camellia and three-nerved spicebush leaves can reduce TC, TG, and LDL-C levels of serum and increase HDL-C level of the serum, the ampelopsis grossedentata leaves can also improve serum SOD activity, reduce a liver coefficient and relieve a damage action of high fat to liver cells. The three-nerved spicebush leaves can reduce an arteriosclerosis index AI, a fat coefficient and body weight and relieve fatty degeneration degree of the liver, and lipid droplet formation in the HpeG2 cells is obviously reduced. The ampelopsis grossedentata leaves, golden camellia and three-nerved spicebush leaves coordinately act and are superior to single ampelopsis grossedentata leaf general flavone, single golden camellia total polysaccharide extract and single three-nerved spicebush leaf general flavone in the blood fat reducing effect and has more comprehensive preventing action agaisnt blood fat and blood fat-reducing effect.

The invention discloses a phytosterol compound lipid-decreasing formula and a preparation method for a tablet thereof. The formula comprises the following components in parts by weight: 15-70 parts of phytosterol, 5-50 parts of soybean peptide, 5-30 parts of beta-glucosan, 1-20 parts of lecithin, 10-70 parts of mannite, 1-30 parts of maltodextrin, 0.01-1 part of sucralose and 5-30 parts of microcrystalline cellulose. The preparation method for the tablet thereof comprises the following steps: mixing and stirring; performing wet granulation; drying; stabilizing grain size; and pressing into the tablet, thereby acquiring the tablet of the formula. The invention has the beneficial effects that the formula has an excellent lipid-decreasing effect, is capable of effectively reducing the blood lipid level of hyperlipidemia patient and has a lipid-decreasing healthcare function; the tablet prepared according to the preparation method for the tablet tastes nice; the tablet is high in stability.

The invention discloses an application of naringin in preparing medicaments for curing atherosclerosis. Tests prove that the naringin can decrease level of serum cholesterol, inhibit cholesterin on arterial vascular walls from depositing, obviously decrease level of serum TC, TG, LDL-C, remarkably raise level of serum HDL-C, improve activity of serum SOD and increase level of NO, obviously decrease level of MDA, effectively prevent atheromatous plaques from being formed or damaged, and have the obvious function of curing atherosclerosis.

The present invention relates to a medicine for treating fat live of obesity. It is prepared with seven kinds of Chinese medicinal materials including giant knotwood, pinellia tuber, oriental water plantain, zedoary, peach kernel, prince's-feather fruit and licorice. The preparation process is also disclosed. The medicine has the functions of promoting diuresis, eliminating phlegm, promoting blood circulation and elimianting abdominal mass. It is used to treat fat liver of obesity caused by wetness phlegm stasis mainly. Clinical application shows that it has fast and obviously treating effect and no bad reaction.

The invention discloses application of Xyloketal B in preparing antiatherosclerotic medicaments. Experiments prove that: Xyloketal B has the effects of reducing the plaque area and regulating blood fat on atherosclerotic lesions, and can prevent and treat atherosclerosis by protecting vascular endothelium, regulating the blood fat level such as reducing low-density lipoprotein cholesterol (LDL-C) and raising the LDL-C, resisting oxidative stress in vivo and other action mechanisms. A novel application field of the Xyloketal B is developed when the Xyloketal B is applied to preparing the antiatherosclerotic medicaments, and the Xyloketal B is safe and non-toxic and has strong pharmacological action and good medicinal prospect.

The invention discloses application of a copperleaf herb aqueous extract in preparation of weight-losing and lipid-lowering medicines or weight-losing and lipid-lowering health-care products. A preparation method of the copperleaf herb aqueous extract includes the steps that whole copperleaf herb is collected, cleaned, and dried; the copperleaf herb is crushed to obtain copperleaf herb dry powder;the copperleaf herb dry powder is added into an extraction tank, then pure water is added, and then soaking, decocting, and filtering are conducted; filtrate is collected, filtered solids is decoctedwith the pure water again, filtering is conducted, and the filtrate is collected; the pure water is finally added into the filtered solids, decocting and filtering are conducted, and the filtrate iscollected; and the filtrate from the filtration for three times is combined and concentrated in a rotary evaporator, and a copperleaf herb aqueous extract original liquid medicine is obtained. In-vivoanimal tests show that the copperleaf herb aqueous extract has obvious weight-losing and lipid-lowering effects; the copperleaf herb aqueous extract can also inhibit the contents of TC, TG and LDL-Cin serum of mice, increase the activity of SOD enzymes in livers of the mice, and reduce the content of MDA, so that the antioxidant activity of organisms is high, lipid oxidation is reduced, liver injury is relieved, and the effect of protecting the organisms is achieved.

The invention discloses functional food containing lithocarpus litseifolius leaves and fructus momordicae and a preparation method of the functional food. The functional food is prepared from raw materials in percentage by mass as follows: 20%-80% of lithocarpus litseifolius leaves and 20%-80% of fructus momordicae. The preparation method of the functional food comprises the steps as follows: after the lithocarpus litseifolius leaves and the fructus momordicae are pretreated, ethanol is added for reflux extraction, obtained filtrates are mixed, ethanol is recovered, extract is dispersed in hotwater, standing, centrifugation, precipitation and vacuum drying are performed, maltodextrin is added, a soft material is prepared, granulation, drying and shaping are performed, total granules are obtained, and the functional food is obtained. Research finds that after all raw materials are matched for use, the synergistic effect is played, and the functional food has significant functions of reducing blood sugar and blood lipid.

The invention belongs to the field of biochemistry, and particularly relates to an RNA inhibitor for inhibiting proprotein convertase subtilisin Kexin9 (PCSK9) gene expression in hepatocytes and application thereof. The RNA inhibitor is formed by a positive-sense strand and an antisense strand through base pairing, at least 85% of bases between the positive-sense strand and the antisense strand are complementary, -OH at the 2' site of partial or all nucleotide glycosyls is substituted by fluorine or methoxyl, and phosphate among at least three continuous nucleotides at the tail end is sulfated. The structure of the RNA inhibitor provided by the invention also contains 5' MVIP and 3' MVIP, so that the RNA inhibitor has specific liver targeting. In-vivo and in-vitro pharmacological experiments prove that: the RNA inhibitor directly acts on PCSK9 mRNA, continuously and efficiently inhibits PCSK9 gene expression and reduces the level of LDL-C in plasma, and is used for treating or / and preventing PCSK9 gene mediated diseases including but not limited to hyperlipidemia, atherosclerosis or PCSK9 gene mediated other diseases.

The invention provides application of a mesona chinensis extract in preparation of drugs or health-care products for regulating intestinal flora and relates to the technical field of traditional Chinese medicines. According to the application, experiments prove that the mesona chinensis extract can be used for regulating an intestinal flora structure of a high-fat rat, promoting growth of beneficial bacteria, i.e., akkermansia muciniphila, improving a ratio of firmicutes / bacteroidetes, regulating the content of short-chain fatty acids, improving disorder of the intestinal florae, promoting metabolism of fats and inhibiting accumulation of the fats, thereby playing a role in reducing blood fat and protecting liver.

The invention discloses a malus toringoides leave and sea buckthorn functional food and a preparation method thereof. The malus toringoides leave and sea buckthorn functional food is prepared from thefollowing raw materials in percentage by mass: 20 to 80 percent of malus toringoides leave and 20 to 80 percent of sea buckthorn. The preparation method of the malus toringoides leave and sea buckthorn functional food comprises the following steps: pretreating the malus toringoides leave and the sea buckthorn and then adding ethanol for reflux extraction; combining the obtained filtrate, recovering ethanol, dispersing extract by using hot water, standing, centrifuging, precipitating and carrying out vacuum drying; adding maltodextrin, preparing a soft material, granulating, drying and granulating to obtain total particles, thus obtaining the malus toringoides leave and sea buckthorn functional food. The research on the malus toringoides leave and sea buckthorn functional food disclosed bythe invention shows that after the combination of various raw materials, a synergistic effect is played; the malus toringoides leave and sea buckthorn functional food has obvious effects of loweringblood sugar and lowering blood lipid.

The invention relates to a hypoglycemic active part of osmanthus fragrans, a preparation method and application thereof, and application of the osmanthus fragrans in reducing blood fat, wherein the hypoglycemic active part of the omanthus fragrans is that the osmanthus osmanthus is extracted by petroleum ether, ethyl acetate and normal butanol to prepare petroleum ether, ethyl acetate and normal butanol extracts respectively, wherein the ethyl acetate extract is further separated to prepare triterpene from osmanthus fragrans. An effective component, namely the triterpene from osmanthus fragrans, can be used for preparing natural hypolipidemic and anti-oxidant medicaments; and the osmanthus fragrans of the invention and the effective component thereof, namely the triterpene from osmanthus fragrans, are used for preparing health products for the application of the prevention and adjuvant therapy of hyperlipemia. In vivo animal experiments prove that the triterpene from osmanthus fragranshas the remarkable effect of reducing the level of TC, TG and LDL-C in serum of mice with the hyperlipemia, and also has the effect of reducing MDA and increasing the activity of SOD at the same time. Therefore, the osmanthus fragrans not only can reduce the blood lipid level in the serum by improving the autoimmunity, but also can reduce the blood fat by improving the in vivo antioxidant mechanism.

The invention relates to a culture medium for culturing a living biological medicine and application of the culture medium. The culture medium for culturing the living biological medicine is prepared from peptone, beef extract, lactulose, mannose oligomer, raffinose, yeast extract, diammonium hydrogen citrate, K2HPO4, MgSO4, MnSO4, Tween-80 and L-cysteine. The living biological medicine comprises any one or a combination of at least two of lactobacillus reuteri, lactobacillus paracasei, lactobacillus acidophilus, lactobacillus rhamnosus, bifidobacterium longum, pediococcus pentosaceus, lactobacillus crispatus, lactobacillus jensenii or lactobacillus gasseri. The culture medium has an unexpected promoting effect on growth, proliferation, metabolism and efficacy exertion of living biological medicines. The efficacy is such as prevention or treatment of gastrointestinal diseases, immune diseases, cancers, vaginitis and other diseases.

The invention relates to the technical fields of foods, and discloses health-nourishing liquor, as well as a preparation method and application thereof. The health-nourishing liquor is prepared from the following raw materials: raspberry extract, Chinese wolfberry fruits, lily, spina date seeds, hovenia dulcis thunb, sophora flower buds, licorice roots, rock candy and distilled spirit. The novel health protecting and nourishing prescription in accordance with traditional Chinese medicine theory is provided, and is perfectly synergistic with the distilled spirit to achieve an excellent liver protecting effect, and remarkably improve hyperlipemia and other diseases. The invention further provides a method for preparing the health-nourishing liquor, and the method can be used for preparing the health-nourishing liquor with clear and transparent color, no sediment or suspension, synergistic fragrance, mellow taste, smooth and harmonious taste due to scientific designing step and preparation mode of each raw material. The health-nourishing liquor can be widely applied to various health products, and has a wide market prospect.

The invention provides a plant salt containing diosgenin. The plant salt is prepared from a suaeda salsa extract, flos sophorae pollen, pineapple powder, perilla frutescens powder, a herba rhodiolae extract, a fructus rubi extract and diosgenin. A preparation method of the plant salt containing diosgenin comprises the step of suaeda salsa extract preparation which comprises the steps of smashing, heating extraction, decoloring and heavy metal removing. The plant salt containing diosgenin has the advantages that when the plant salt is taken, the obvious blood lipid lowering function is achieved; by means of the experiments, TC, TG and LDL-C levels of a mouse serum are obviously reduced, and the HDL-C level of the mouse serum is improved, the blood pressure of a patient with the high blood pressure is obviously lowered, the effective rate is 93% to 99%, and the obvious effective rate is 80% to 87%; the symptoms of constipation of the patient are obviously improved, and the effective rate of improving constipation is 84% to 92%; no bad symptom causing body discomfort exists.

The invention discloses a lithocarpus litseifolius and emblic leafflower fruit functional food and a preparation method thereof. The lithocarpus litseifolius and emblic leafflower fruit functional food comprises the following raw materials by weight percent: 20%-80% of lithocarpus litseifolius and 20%-80% of emblic leafflower fruit. The preparation method thereof comprises the following steps: pretreating lithocarpus litseifolius and emblic leafflower fruit, and then adding ethyl alcohol for reflux extraction; mixing the acquired filtrate; recycling ethyl alcohol; dispersing extract in hot water; standing by; centrifuging; vacuum-drying precipitation; adding maltodextrin; preparing a soft material; pelletizing; drying; stabilizing size, thereby acquiring total particles. Through research,the invention finds that a synergistic effect is achieved after all the raw materials are combined and used together, and the effects of reducing blood sugar and reducing blood fat are obvious.

A rosemary-containing composition, a preparing method thereof and applications of the composition are disclosed. The composition includes, by weight, 20-100 parts of rosemary, 10-20 parts of phytosterol, 10-30 parts of tomato seed oil and 10-30 parts of diacylglycerol. The composition is used for adjusting human body blood fat metabolism. The rosemary, the phytosterol, the tomato seed oil and the diacylglycerol which are raw materials are free of incompatibility so that safety can be fully ensured. The composition is adopted as a diet control means, and after the composition is eaten, through adjusting human body blood fat metabolism, occurrence and development of dyslipidemia can be prevented and treated, the risk of atherosclerotic cardiovascular and cerebrovascular diseases is reduced, and quality of life of people with some diseases is improved.

The invention discloses folium mali hupehensis and fructus Lycii ruthenici functional food and a preparation method thereof. The functional food contains, by mass, 20%-80% of folium mali hupehensis and 20%-80% of fructus Lycii ruthenici. The preparation method includes: after pretreatment of folium mali hupehensis and fructus Lycii ruthenici, adding ethanol for reflux extraction, combining filtrates, recovering ethanol, dispersing extract in hot water, standing, centrifuging, precipitating, performing vacuum drying, adding maltodextrin, preparing a soft material, granulating, drying and finishing to obtain total particles, so that the functional food is obtained. According to researches, by compatible utilization of the raw materials, synergistic effects are achieved, and blood sugar and blood fat reduction effects are remarkable.

The invention provides an application of the jelly jelly extract in the preparation of medicines or health care products for regulating intestinal flora, and relates to the technical field of traditional Chinese medicine. The present invention proves through experiments that the jelly jelly extract can regulate the intestinal flora structure of high-fat rats, promote the growth of beneficial bacteria Ekmansia, improve the ratio of Firmicutes / Bacteroidetes, regulate the content of short-chain fatty acids, and improve intestinal Improving the imbalance of intestinal flora, promoting fat metabolism and inhibiting fat accumulation, so as to lower blood fat and protect liver.

The invention discloses functional food containing dry Malus toringoides and Malus transitoria leaves and Zea mays styles and stigma and a preparation method of the functional food. The functional food comprises raw materials in percentage by mass as follows: 20%-80% of the dry Malus toringoides and Malus transitoria leaves and 20%-80% of the Zea mays styles and stigma. According to the preparation method of the functional food, the dry Malus toringoides and Malus transitoria leaves and the Zea mays styles and stigma are pretreated, ethanol is added for reflux extraction, obtained filtrates are mixed, ethanol is recovered, extract is dispersed in hot water, left to stand and centrifuged, then precipitates are dried in vacuum, maltodextrin is added, a soft material is prepared, granulation,drying and pelletizing are performed, and total pellets are obtained. Study finds that the raw materials have synergy after being used concomitantly, and the functional food has remarkable effects ofreducing blood sugar and reducing blood lipid.

The invention discloses an exosome of an LDLR mutant, a preparation method of the exosome, and an application of the exosome in preparing drug against hyperlipidaemia, and relates to the technical field of drugs for treating dyslipidemia. The preparation method comprises the following steps: inoculating a medium containing fetal bovine serum with hepatocytes, transfecting the hepatocytes with adenoviruses over-expressing a low-density lipoprotein receptor gene mutant that is terminating mutation caused by mutation of a single base in an O-linked sugar chain domain of the low-density lipoprotein receptor gene; then replacing the serum-free medium and continuously conducting culturing after transfection, and collecting supernatant; centrifuging the supernatant to remove floating living cells, thereby obtaining the supernatant containing the exosome; and condutcing separating and purifying to obtain the cell exosome over-pressing the low-density lipoprotein receptor gene mutant. The exosome of the present invention is a natural vector produced by an endogenous cell. The exosome is used as a transport vector, and has the unique advantages of low immunogenicity, high transport efficiency, good stability, high targetability and ability to pass the blood-brain barrier.

The invention discloses malus toringoides leaf momordica grosvenori functional food and a preparation method thereof. The malus toringoides leaf momordica grosvenori functional food is prepared from the following raw materials in percentage by mass: 20 to 80 percent of malus toringoides leaves and 20 to 80 percent of momordica grosvenori. The preparation method comprises the following steps of pretreating malus toringoides leaves and momordica grosvenori; adding ethanol; performing backflow extraction; merging the obtained filter liquid; recovering ethanol; performing hot water dispersion on extracts; performing standing, centrifugation and precipitation vacuum drying; adding maltodextrin; making soft materials; performing granulation, drying and particle sorting to obtain total particles;obtaining a final product. Through study, the conditions that after the matched use of all raw materials, the synergistic interaction effect is achieved, and the blood sugar and blood fat reducing effects are obvious are discovered.

The invention discloses an application of Symplocos chinensis polysaccharides in the preparation of medicines for preventing and treating hyperlipidemia and hypertension. The Symplocos chinensis polysaccharides can be used in the preparation of medicinal preparations for preventing and treating hyperlipidemia and hypertension as one of active components or as the only one active component. The Symplocos chinensis polysaccharides can effectively reduce the content of total cholesterol (TC), triglycerides (TG) and LDL-C in hyperlipidemia mouse serums, and have active effects on fat metabolism adjustment and AS prevention. The HDL-C transports cholesterols in blood to the liver, and converts the cholesterols into bile acid or makes the cholesterols be directly excreted through bile. Obvious increase of the HDL-C in Symplocos chinensis polysaccharide group mice shows that the Symplocos chinensis polysaccharides can promote transportation of the cholesterols in the blood to the liver and promote conversion and drainage in order to reduce the cholesterols in the blood and reduce rises of the coronary heart disease and atherosclerosis.

The invention belongs to the technical field of traditional Chinese medicine and especially relates to a compound with blood lipid-reducing and oxidation-resisting effects. The compound with blood lipid-reducing and oxidation-resisting effects is characterized in that the compound is prepared from the following raw materials in parts by weight: 3-20 parts of Huashan alum polysaccharide, 3-25 parts of fleece-flower roots, 3-25 parts of red sage roots, 3-25 parts of hawthorn fruits, 3-25 parts of Gynostemma pentaphylla, 5-30 parts of semen cassiae torae, 8-35 parts of chrysanthemum flowers and 8-35 parts of Chinese wolfberry fruits. The compound is prepared into tablets, capsules, pills, granules and injections by conventional processes. The Huashan alum polysaccharide resources are fully utilized in the compound, and the medicinal uses of the Huashan alum are developed; moreover, the developed Huashan alum polysaccharide has good effects of reducing blood lipid as well as good antioxidant activity and low adverse reaction rate. Curative effects of the medicament prepared from the compound for treating hyperlipidemia are better than the curative effects of the single use of the Huashan alum polysaccharide for treating hyperlipidemia in rats.

The invention discloses functional food containing malus hupehensis leaves and emblic leafflower fruits and a preparation method of the functional food. The functional food comprises raw materials inpercentage by mass as follows: 20%-80% of malus hupehensis leaves and 20%-80% of emblic leafflower fruits. The preparation method of the functional food comprises the steps as follows: pre-treating the malus hupehensis leaves and the emblic leafflower fruits, then adding ethanol, performing reflux extraction, mixing obtained filtrates, recovering the ethanol, dispersing an extract by hot water, performing still standing, centrifugation, precipitation and vacuum drying, adding maltodextrin, preparing a soft material, and performing granulation, drying and granulation to obtain total granules toobtain the functional food. Research finds that after compatible use of all raw materials, the functional food plays a synergistic effect and has significant blood sugar and blood lipid reduction functions.