The invention discloses a plant essence formula capable of effectively promoting hair growth and a preparation method thereof. The plant essence formula comprises: fleece-flower roots, herba ecliptae,cacumen biotae, folium mori, radix sophorae flavescentis, fructus cnidii, glossy privet fruit, cortex moutan, black bean coat, angelica sinensis, radix rehmanniae, radix rehmanniae preparata, radix salviae miltiorrhizae, lavender, liquorice, rhizoma zingiberis and folium llicis latifoliae. The preparation method comprises the following steps: (1) crushing and uniformly mixing plant raw materialsto obtain plant powder; (2) adding white spirit into the plant powder, and performing soaking and filtering to obtain a plant essence leaching solution; (3) heating and extracting the plant essence leaching solution to obtain a plant essence concentrated solution; and (4) stirring and filtering the plant essence concentrated solution to obtain the plant essence. The plant essence formula takes pure traditional Chinese medicine plants as raw materials, is safe, non-toxic and free of side effects, has the effects of nourishing scalp, repairing hair follicles, eliminating dandruff and effectivelypromoting hair growth, can be directly smeared on scalp, does not need secondary cleaning, and is convenient to use.