The invention relates to a preparation method of congenital qi-strengthening paste for females. The congenital qi-strengthening paste consists of nuts, almonds, grapes, colla corii asini, longan aril, yellow wine and crystal sugar. The preparation method comprises the preparation processes: firstly, baking the nuts, the almonds and the grapes at low temperature until the nuts, the almonds and the grapes are cooked completely, pulping the longan aril to obtain juice, then mixing the cooked nuts, the cooked almonds, the cooked grapes and the colla corii to obtain a mixture, crushing the mixture to obtain mixed materials, and finally mixing the mixed materials, the longan aril juice and the yellow wine together to obtain a new mixture; and then, cooking the new mixrture until the mixed materials are completely cooked so as to obtain the finished products of the congenital qi-strengthening paste. Through the implementation of the preparation method disclosed by the invention, the raw materials are baked at the low temperature, so that the nutrients of the raw materials can be guaranteed not to lose, and besides, the congenital qi-strengthening paste does not have peculiar smell and is rich in fragrance; after the congenital qi-strengthening paste is eaten, people do not suffer from excessive internal heat. Besides, the prepared congenital qi-strengthening paste is especially suitable for females to eat for a long term, has a very good cosmetic effect, can enable hairs to be jet-black, is rich in light and elasticity, reduces splashes and wrinkles of faces, and has a favorable blood replenishing effect, so that the congenital qi-strengthening paste can delay aging. Besides, the congenital qi-strengthening paste is suitable for females suffering from dysmenorrhea, lying-in women to supplement qi and blood, and ready-to-be mothers to replenish blood, nourish the kidney, loosen bowel to relieve constipation, ease the mind and the like.