The invention provides a one-step forming reflective silk screen printing ink and a production method thereof, relating to the technical field of ink and silk screen printing. The production method comprises the following steps: mixing resin with solid content of 100%, a plasticizer, an anti-sedimentation agent, transparent pigment, an organic solvent and aluminum powder paste, and grinding until a mixed material with fineness less than 25 mu m and viscosity large than or equal to 140 S is obtained; and adding reflective microspheres with fineness above 350 meshes, and evenly stirring, wherein in the mixing process, the masses of the resin with solid content of 100%, the plasticizer, the anti-sedimentation agent, the transparent pigment, the organic solvent, the aluminum powder paste and the reflective microspheres respectively account for 15-45%, 0.5-4%, 1-4%, 2-8%, 10-36%, 1-20% and 30-70% of the total mass of the ink. The operation is simple, and the method is especially suitable for large-scale production of reflective signs with the same pattern; the ink can be applied to the surfaces of materials such as plastics, metals, clothes and the like; and when being irradiated by lamplight at night, the bright reflective signs can be provided, thereby effectively reducing the loss of life and property.