45 results about "Coccygeal vein" patented technology

The invention provides a benzodiazepine compound as well as a preparation method and a pharmaceutical effect thereof, and belongs to the field of chemical medicines. The benzodiazepine compound is a compound shown as a formula I, or a salt thereof, or a stereoisomer thereof, or a solvate thereof, or a eutectic thereof, or a composition thereof. The compound is good in intravenous sedative anesthesia effect, the anesthesia effect of the compound is equivalent to or even superior to that of remimazolam, and specifically, the effective dosage is remarkably reduced, and the duration time and the recovery time are remarkably shortened. Meanwhile, in rat and mouse caudal vein anesthesia models, compared with remimazolam, the compound has remarkably improved waking quality. The compound providedby the invention has the advantages of fast onset, short duration, fast awakening and good tolerance during anesthesia, can be used for anesthesia induction, anesthesia maintenance and daytime operation anesthesia, and has a good application prospect.

A mouse tail vein blood collection and injection device, comprising a mouse cabin and a platform, the mouse cabin includes a first arc plate, a second arc plate, a first horizontal plate, a second horizontal plate, a first vertical plate, a second vertical plate plate, the first slide rail, the first arc plate is vertically set, the first vertical plate is vertically set, and is connected with the side of the first arc plate and the first horizontal plate at the same time, the second arc plate is set in the mirror image of the first arc plate, and the second The second horizontal plate is connected to the top of the second arc plate, the second vertical plate is vertically arranged, and is connected to the side of the second arc plate and the second horizontal plate at the same time, the second horizontal plate is provided with a first slide rail, and the second horizontal plate passes through The first slide rail is movably connected with the first horizontal plate. The structure design of the present invention is reasonable, and the space adjustment of the device mouse cage can be carried out by pulling and sliding, and the mice of different sizes can be well fixed. At the same time, a lighting system is provided, and yellow light lighting is used for assistance, making it easier to find the tail vein and improve the accuracy. , more convenient and scientific to use.

The invention discloses a method for constructing an immunodeficiency mouse transplant model of human stem cells. The method comprises the following steps of: performing intraperitoneal injection on a receptor mouse (NOD / SCID) by adopting an N-acetylcysteine (NAC) antioxidant before transplanting, supplying drinking water containing NAC to the receptor mouse, continuously supplying the drinking water containing the NAC to the receptor mouse after the human stem cells are transplanted, and thus obtaining the immunodeficiency mouse transplant model of the human stem cells. The optimal model of NOD / SCID mouse transplant is successful established; and by the method, no matter in caudal vein or medullary cavity transplant, the implantation rate of the human stem cells is effectively improved, and the mouse is low in death rate and good in status in the transplant process and can survive for a long term. The method is simple, novel, easy to implement and low in wound to the mouse; and the NAC antioxidant used by the method is low in price.

The invention discloses a construction method and application of a gout animal model. The construction method comprises daily intraperitoneal injection of uricase inhibitor to rats for 1 week to inhibit the activity of rat uricase, and then simultaneous injection of urate crystals and ATP into tail vein to make rats spontaneously develop gouty arthritis. The gout animal model construction method provided by the invention can stably and rapidly induce gout in rats, and the clinical manifestations and pathological characteristics of the model are completely consistent with the characteristics of human gout. In the gout model constructed by this method, a large number of urate crystals can be observed under a polarizing microscope in the diseased joints, and a large number of neutrophil infiltration can be found by immunohistochemistry. In addition, the present application also relates to the application of the gout animal model constructed by the method in the research of the mechanism of occurrence and relief of gout and related inflammation, screening and evaluation of therapeutic drugs, and the like.

The invention discloses a mouse tail vein blood collection system, which comprises a table body, the table body includes a horizontal plane and an inclined plane, centrifugal equipment is arranged on the horizontal plane, and rat body fixing components, The mouse tail positioning device and the mouse tail clamping assembly, the mouse tail positioning device includes a channel for the mouse tail to pass through; it also includes a platform on an inclined surface, the upper surface of the platform is parallel to the horizontal plane, and the bearing There is a socket for placing the EP tube on the surface of the platform. The invention provides a mouse tail vein blood collection system to solve the problems in the prior art that it is difficult to ensure the stability of the tail of the blood to be taken during the mouse tail vein blood collection process, and it is easy to mix air bubbles in the blood sample to interfere with the follow-up experiment. Rat tail vein blood sampling for the purpose of stability and continuity.

The invention discloses a method for isolating and culturing primary hepatocytes of bream. Healthy juvenile bream with a body weight of 30‑50 g were selected, and the whole body was disinfected with 1% potassium permanganate. After blood was collected from the tail vein, the liver was dissected aseptically. After digestion with collagenase, the cell suspension was filtered through a 200-mesh cell sieve, and excess red blood cells in liver cells were removed by red blood cell lysate, and cell debris was removed by gradient centrifugation. Add an appropriate amount of complete medium to the obtained cell pellet to suspend the cells, then use a cell counter to calculate and adjust the cell concentration, after plating, put it in 28°C, 5% CO 2 cultured in a cell incubator, and observed the adherence after 48 hours. The invention innovatively combines the characteristics of the researched species to isolate and cultivate hepatocytes, and the obtained cell viability is as high as 90%, which meets the requirements of primary culture, and provides theoretical basis and technical support for further experiments related to primary hepatocytes of bream .

The application of N6022 in the treatment of aortic dissection and aortic aneurysm involves the cardiovascular field. In mouse models of aortic dissection and aortic aneurysm, tail vein injection of N6022 can inhibit the expansion of the aorta, reduce the incidence and mortality of arterial dissection and aortic aneurysm; and N6022 can reduce the rupture of aortic aneurysm, inhibit The occurrence of hematoma in the inner wall of the lumen and the excessive collagen deposition on the vessel wall maintain the integrity of the elastic fibers of the vessel wall and treat aortic dissection and tumor diseases. The invention opens up a new application field of N6022, and provides a meaningful reference for preventing and treating aortic dissection and aortic aneurysm diseases and improving vascular pathological changes.

A tail vein injection method for mice, involving an injection method, the tightening bolt (24) is screwed counterclockwise, the clamping of the U-shaped splint (12) is loosened, and the device (1) is rotated clockwise by hand to make the device ( 1) In a vertically placed state, turn the tightening bolt (24) clockwise to clamp and fasten the U-shaped splint (12) and the vertical plate (23), and fix the position of the device (1); the invention discloses A mouse tail vein injection method was developed. During the pharmacological experiment, it can realize the immobilization of mice that meet different specifications and body weights, and the integration of mouse placement and injection process. The body weight is limited and the mouse needs to be "stuck" for the inconvenience of filling. The invention is convenient to operate and optimizes the mouse filling method and the tail vein injection operation of the mouse.