45 results about "Coccygeal vein" patented technology

The invention belongs to the fields of medical evaluation, detection technology and experimental zoology, and specifically relates to a construction method of animal model of rat which is used for screening medicaments for treating chronic inflammation, and evaluating methods for treating systematic chronic inflammation. The method comprises the following step that healthy male SD rats are administered with lipopolysaccharide by tail intravenous injection, on an every first day of a week for 4-8 weeks persistently, at a dosage of 120-300 [mu]g per kg of body weight, so that pathologic changes, which meet pathological characteristics of chronic inflammation, mainly of an increased ratio of peripheral blood leucocyte count to neutrophile granulocyte, an increased serum hypersensitive C-creative protein level, infiltration of heart, liver, lung, kidney in inflammatory cells, and haemostasis and oedema of tissues are generated. The method has a good repeatability, and operation of the method is simple, easily controllable and mechanism-clear, besides indexes can be analyzed quantitatively and are convenient for statistics treatments. In addition, the constructed animal model is more ideal and more conform to clinic pathological and physiological changes of the type of disease.

The invention discloses application of N6022 in treatment of aortic dissection and aortic aneurysm, and relates to the field of cardiovascular diseases. In mouse models of aortic dissection and aorticaneurysm, dilation of aorta can be inhibited after intravenous injection of the N6022 into tail veins, thereby reducing incidence and mortality of aortic dissection and aortic aneurysm; and moreover,the N6022 is capable of reducing rupture of aortic aneurysm, inhibiting occurrence of hematoma in inner walls of lumen and excessive collagen deposition on vascular walls, maintaining integrity of elastic fibers in the vascular walls, and treating the aortic dissection and the aortic aneurysm. The application of the N6022 in the treatment of the aortic dissection and the aortic aneurysm opens upa new application field of the N6022, and provides a meaningful reference for the prevention and the treatment of the aortic dissection and the aortic aneurysm diseases as well as improvement of vascular lesions.

The invention relates to an application of recombinant ganoderma lucidum immunomodulatory protein rLZ-8 in treating lung cancer, laryngocarcinoma and brain glioma. According to the invention, the in-vivo anti-tumor effect of the rLZ-8 is researched by respectively building a lung cancer mice model by virtue of a caudal vein injection method and building a laryngocarcinoma and brain glioma nude mice animal model by virtue of a subcutaneous transplantation tumor method; a treatment result indicates that the rLZ-8 achieves an obvious anti-tumor effect on the lung cancer, the laryngocarcinoma and the brain glioma.

The invention discloses an establishing method of a hypertensive disorder complicating pregnancy mouse model. The establishing method of the hypertensive disorder complicating pregnancy mouse model comprises the following steps of: extracting a syncytiotrophoblast microvilli membrane; and inputting the syncytiotrophoblast microvilli membrane to a pregnant mouse body of the same strain by caudal vein injection to establish the hypertensive disorder complicating pregnancy mouse model. The hypertensive disorder complicating pregnancy mouse model established by the establishing method disclosed by the invention has hypertensive disorder complicating pregnancy characteristic symptoms and signs including elevation of blood pressure, eclampsia hyperspasmia, damages to heart and liver and the like, which are consistent with the symptoms and pathological process of the hypertensive disorder complicating pregnancy including eclampsia and the like at the early stage of the human eclampsia, and therefore, the hypertensive disorder complicating pregnancy mouse model can be used for researching the occurrence and development mechanism of the hypertensive disorder complicating pregnancy including eclampsia and the like at the early stage of the eclampsia, as well as the medical foundation with the purpose of developing antagonistic medicaments and the clinical research.

The invention discloses a method for constructing an immunodeficiency mouse transplant model of human stem cells. The method comprises the following steps of: performing intraperitoneal injection on a receptor mouse (NOD / SCID) by adopting an N-acetylcysteine (NAC) antioxidant before transplanting, supplying drinking water containing NAC to the receptor mouse, continuously supplying the drinking water containing the NAC to the receptor mouse after the human stem cells are transplanted, and thus obtaining the immunodeficiency mouse transplant model of the human stem cells. The optimal model of NOD / SCID mouse transplant is successful established; and by the method, no matter in caudal vein or medullary cavity transplant, the implantation rate of the human stem cells is effectively improved, and the mouse is low in death rate and good in status in the transplant process and can survive for a long term. The method is simple, novel, easy to implement and low in wound to the mouse; and the NAC antioxidant used by the method is low in price.

The invention discloses a thrombolytic efficacy evaluation method based on a two-photon microscope technology. The thrombolytic efficacy evaluation method comprises the following steps: (1) establishing a cerebral thrombosis animal model of a living animal in a waking state, wherein the cerebral thrombosis animal model comprises an arterial thrombosis model and a venous thrombosis model; (2) carrying out fluorescence labeling on a thrombolytic drug by adopting fluorescein isothiocyanate to obtain a fluorescein isothiocyanate labeled thrombolytic drug; (3) injecting the fluorescein isothiocyanate labeled thrombolytic drug into the caudal vein of the cerebral thrombosis animal model; (4) detecting hemodynamics and vascular morphological changes after thrombolytic drug treatment, and observing changes of blood oxygen pressure after thrombolytic drug treatment; (5) observing the change of the cerebral infarction area after thrombolytic drug treatment, and judging the cerebral area range affected by thrombus; and (6) judging the efficacy of the thrombolytic drug according to the result. The method has the characteristic that the efficacy of the thrombolytic drug can be directly evaluated.

The invention discloses a construction method and application of a gout animal model. The construction method comprises the steps of injecting a uricase inhibitor into the abdominal cavity of a rat every day for one week to inhibit the activity of uricase of the rat and meanwhile injecting uric acid salt crystals and ATP into the tail vein to make the rat spontaneously develop gouty arthritis. Theprovided construction method of the gout animal model can stably and rapidly induce gout in the rat, and the clinical manifestation and pathological characteristics of the model are completely matched with the characteristics of human gout. According to the gout model constructed by means of the method, massive uric acid salt crystals can be observed under a polarizing microscope in a diseased joint, and infiltration of massive neutrophilic granulocytes can be found through immunohistochemistry. In addition, the invention also relates to the application of the gout animal model constructed bymeans of the method in research on the occurrence and remission mechanisms of gout and related inflammation, screening and evaluation of therapeutic drugs and the like.

The invention discloses a rat caudal vein blood sampling device, which is characterized by comprising a bottom plate, a connecting rotating shaft, a cover body, an operation table, a storage drawer, amouse locking cage, a mouse locking cage rotating shaft, a rotating shaft base, a tail fixing table, a supporting connecting column, a guide rail, a centrifugal machine body, a centrifugal machine cover, a blood drawing tube, a vacuum blood vessel bottle and a constant-temperature water bath tube. According to the device, a rat fixing device, a tail fixing device, a heating device, a blood sampling device and a centrifugal machine are integrated, all steps are seamlessly connected when the device is used, and the efficiency is higher; and the rat caudal blood vessel filling adopts dual effects of electric heating and compression, so that the effect is more obvious.

The invention provides a benzodiazepine compound as well as a preparation method and a pharmaceutical effect thereof, and belongs to the field of chemical medicines. The benzodiazepine compound is a compound shown as a formula I, or a salt thereof, or a stereoisomer thereof, or a solvate thereof, or a eutectic thereof, or a composition thereof. The compound is good in intravenous sedative anesthesia effect, the anesthesia effect of the compound is equivalent to or even superior to that of remimazolam, and specifically, the effective dosage is remarkably reduced, and the duration time and the recovery time are remarkably shortened. Meanwhile, in rat and mouse caudal vein anesthesia models, compared with remimazolam, the compound has remarkably improved waking quality. The compound providedby the invention has the advantages of fast onset, short duration, fast awakening and good tolerance during anesthesia, can be used for anesthesia induction, anesthesia maintenance and daytime operation anesthesia, and has a good application prospect.

The invention relates to a method for establishing a rat cerebral hemorrhage model by combining ultrasound with microbubbles, and belongs to the technical field of biological model preparation. The method is implemented by the following steps of 1) taking one SPF-grade male SD rat with the body mass of 240-270 g; 2) making preparation work; 3) with the help of an auxiliary tool, injecting a microbubble contrast agent from the tail vein of the rat, wherein the injection amount is determined according to the body mass of the rat; 4) performing ultrasonic intervention; 5) carrying out CT plain scanning on the head of the rat; 6) irradiating the rat with an incandescent lamp for at least 2 hours, and keeping the anal temperature at 37+ / -0.5 DEG C; and 7) scoring the neurological function of the rat, and feeding and observing the rat for later use. According to the method, the contrast agent is ultrasonically targeted, and local blood vessels in an intracerebral target area are destroyed byvirtue of the instantaneous cavitation effect of the microbubbles, so that the rat has an intracerebral parenchymal hemorrhage phenomenon, and the research requirement of spontaneous cerebral hemorrhage is met; the method has the advantages of noninvasiveness, simplicity, convenience and easiness in popularization and is a proper reproducible biological modeling method; and meanwhile, auxiliary equipment is introduced, so that the operation is simpler, more convenient and more feasible.

Provided is a method for manufacturing a pig ear intravenous injection model based on mathematical modeling. The method comprises the steps that firstly, agentia is prepared, and the caudal vein is separated; then the prepared agentia is utilized for manufacturing a pig ear mold and an ear vein silicone rubber model, the ear vein silicone rubber model is embedded into an ear vein groove of the pig ear mold, and wrapped by a skin model, and the pig ear intravenous injection model can be manufactured. The method is simple, cost is low, the manufactured ear intravenous injection model is easy to store, complete and attractive in structure, clear and natural in form position, and capable of being used for repeated practice, the defect that live pig ear intravenous injection cannot be repeatedly operated is overcome, the problems that a beginner usually injects a pig repeatedly, and repeated ear intravenous injection cannot be carried out on a live pig are solved, the teacher teaching time is shortened, the learning interest of students is stimulated, and the teaching effect is improved.

The invention discloses application of 2-methoxyestradiol (2ME2) in treatment of systemic lupus erythematosus. 2ME2 can be used as a therapeutic medicine for systemic lupus erythematosus or a component contained in the therapeutic medicines. The systemic lupus erythematosus includes cutaneous lupus, lupus nephritis, lupus encephalopathy and other systemic or / and organic lupus diseases. 2ME2 is given in a tail vein injection manner with a dosage of 0.75 mu M / kg each time, or is given in an intragastric manner with a dosage of 100mu M / kg each time. 2ME2 is used for treating B6.MRL-Fas(lpr) mice. When used for treating lupus erythematosus model mice (MRL-Fas(lpr) mice), 2ME2 has the beneficial effects of lowering the autoantibody level of lupus erythematosus, reducing syndromes of nephritis related to lupus, and relieving lupus syndromes.

A mouse tail vein blood collection and injection device, comprising a mouse cabin and a platform, the mouse cabin includes a first arc plate, a second arc plate, a first horizontal plate, a second horizontal plate, a first vertical plate, a second vertical plate plate, the first slide rail, the first arc plate is vertically set, the first vertical plate is vertically set, and is connected with the side of the first arc plate and the first horizontal plate at the same time, the second arc plate is set in the mirror image of the first arc plate, and the second The second horizontal plate is connected to the top of the second arc plate, the second vertical plate is vertically arranged, and is connected to the side of the second arc plate and the second horizontal plate at the same time, the second horizontal plate is provided with a first slide rail, and the second horizontal plate passes through The first slide rail is movably connected with the first horizontal plate. The structure design of the present invention is reasonable, and the space adjustment of the device mouse cage can be carried out by pulling and sliding, and the mice of different sizes can be well fixed. At the same time, a lighting system is provided, and yellow light lighting is used for assistance, making it easier to find the tail vein and improve the accuracy. , more convenient and scientific to use.

The invention relates to a mouse operation and optical imaging experiment device. The mouse operation and optical imaging experiment device comprises an operation table, a fixing part, a nose mask, an eye mask, a scalp spreader and a caudal vein injection auxiliary device, wherein the operation table is used for placing a mouse; the fixing part is used for accommodating four limbs and the tail of the mouse and realizing flexible fixation of the mouse together with the operation table; the nose mask is used for gas anesthesia of the mouse; the eye mask is used for protecting eyes of the mouse and can be used independently; the scalp spreader is used for fully exposing the brain operation view of the mouse; and the caudal vein injection auxiliary device is connected with the operation table and used for assisting puncture and injection of the caudal vein of the mouse. By means of the mouse operation and optical imaging experiment device, mouse fixation, anesthesia, caudal vein puncture administration and other experiment operations including operation and imaging can be uniformly and smoothly completed on the device, good eye protection and body temperature maintenance can be provided for the mouse, so that the experiment operations are facilitated, the experiment success rate is increased, the research range is expanded, the experiment cost is saved, and animal welfare is fully enhanced.

The invention discloses a method for constructing an immunodeficiency mouse transplant model of human stem cells. The method comprises the following steps of: performing intraperitoneal injection on a receptor mouse (NOD / SCID) by adopting an N-acetylcysteine (NAC) antioxidant before transplanting, supplying drinking water containing NAC to the receptor mouse, continuously supplying the drinking water containing the NAC to the receptor mouse after the human stem cells are transplanted, and thus obtaining the immunodeficiency mouse transplant model of the human stem cells. The optimal model of NOD / SCID mouse transplant is successful established; and by the method, no matter in caudal vein or medullary cavity transplant, the implantation rate of the human stem cells is effectively improved, and the mouse is low in death rate and good in status in the transplant process and can survive for a long term. The method is simple, novel, easy to implement and low in wound to the mouse; and the NAC antioxidant used by the method is low in price.

The invention discloses an anti-tumor nano preparation and application thereof in targeted therapy of malignant tumors. Polydopamine nanoparticles are used as a photothermal conversion material to prepare the anti-tumor nano preparation with high photothermal conversion efficiency, good in-vivo degradability, good biocompatibility and almost no cytotoxicity; meanwhile, the therapeutic effect of killing tumor cells is achieved in combination with photothermal therapy. After the preparation is subjected to tail vein injection into the body of a BALB / C female mouse inoculated with 4T1-luc cells,the photothermal therapy is carried out, the treatment effect is obviously superior to that of conventional treatment, the treatment mode is small in wound area and almost free of toxic or side effects, the administration dosage is reduced, and compared with surgical treatment, the pain is reduced.

The present invention discloses a method of preparing macrophages expressing mIL4 protein. The method comprises the steps: enabling macrophages to express mIL4 protein so as to obtain macrophages expressing the mIL4 protein. According to the steps of the method, the inventor prepares RAW264.7 cells infected with lentivirus pCDH-mIL4. Experiments show that the survival rate of mice is significantlyincreased after the injection of the RAW264.7 cells infected with the lentivirus pCDH-mIL4 into the caudal vein of mouse septicopyemia model; and the survival rate of the mice is also significantly increased after the injection of the RAW264.7 cells infected with the lentivirus pCDH-mIL4 into the trachea of mice with acute lung injury. Therefore, the RAW264.7 cells infected with the lentivirus pCDH-mIL4 can treat septicopyemia and acute lung injury. The RAW264.7 cells have important application value.

The invention discloses a mouse tail vein blood sampling and injection clamp. The mouse tail vein blood sampling and injection clamp comprises a fixing device and a handle device; the fixing device comprises magnetic fixing plates, a fixing opening and a bolster sheet, the magnetic fixing plates can fix the mouse tail to a magnetic panel or a metal plate, the fixing opening is located between a connecting shaft and the front-end magnetic fixing plates, and the rubber bolster sheet is placed in an inner groove of the fixing opening, and is thin at front and thick at back in the horizontal direction of the inner groove; the handle device comprises a spring sheet, handles and the connecting shaft, and the spring sheet is fixed to the inner sides of the handles and used for pressurizing the handles. The mouse tail vein blood sampling and injection clamp has the advantages that that the mouse tail vein blood sampling and injection clamp is simple in structure, small in size and convenient to carry; the rubber bolster sheet is arranged in the clamp and is thin at front and thick at back in the horizontal direction of the inner groove, which conforms to the physiological curvature of themouse tail; the clamp is convenient to operate, and capable of fixing the mouse tail from multiple angles and quickly filling the vein of the mouse tail, tail vein puncturing is facilitated, the timeis saved, the labor can also be saved, and the mouse tail vein blood sampling and injection success rate is increased.

The invention provides a mouse tail vein injection method, and relates to the injection method. The method comprises the following steps: a turning bolt (24) is twisted counterclockwise, clamping of aU-shaped camp plate (12) is loosened, a placement tool (1) is rotated clockwise by hands to make the placement tool (1) in a vertical placement state, the turning bolt (24) is twisted clockwise to clamp and fasten the U-shaped clamp plate (12) and a vertical plate (23), so that the position of the placement tool (1) is fixed. According to the mouse tail vein injection method disclosed by the invention, in pharmacological experiments, the fixation of mice with different specifications and weights and the integration of mouse placement and injection process can be met, and the inconvenience that a fixed device has limit to a mouse weight and mice need to be squeezed when the mice are packed in traditional pharmacological experiments is solved; and the method is convenient to operate, and optimizes a mouse packing manner and the mouse tail vein injection operation.

The invention discloses a human intron derived 27 base microRNA and an application thereof to blood pressure regulation. The human intron derived 27 base microRNA is generated by four-time duplicationof a fourth intron 27 base duplicon of eNOS (endothelial nitric oxide synthase), and the nucleotide sequence of the human intron derived 27 base microRNA is as shown in SEQ ID NO. 1. A high-expression plasmid is prepared according to the sequence, the high-expression plasmid and lipidosome are mixed according to the weight ratio of 3:1 and diluted by normal saline, and mixture is injected into the body of an SD (sprague-daw) rat through rat caudal veins to prove that the human intron derived 27 base microRNA has a regulating effect on rat blood pressure, can be used for researching hypertension diseases, finally can be used for screening some antihypertensive drugs and preparing a hypertension early warning chip and has a potential application prospect.

The invention relates to a preparation and detection method of pancreatic regenerating protein PSP / reg. The preparation and detection method specifically comprises the steps that an adult healthy malesprague-dawley (SD) rat is adopted and treated with a single injection of dibutyltin dichloride in caudal vein to construct a chronic pancreatitis rat model, high fructose is given for feeding, a type 3c diabetes (T3cDM) rat model is established, and then detection of related physiological and biochemical indexes is performed. It is verified that the clinical physiological and biochemical changesof the T3cDM rat model established are consistent with the clinical physiological and biochemical changes of T3cDM. The rat model can establish an experimental basis for the drug screening and clinical treatment of T3cDM and provides experimental tools. Meanwhile, the pancreatic regenerating protein PSP / reg further provides a good experimental gist for the diagnosis or treatment of T3cDM.

The invention discloses a mouse tail vein blood collection system, which comprises a table body, the table body includes a horizontal plane and an inclined plane, centrifugal equipment is arranged on the horizontal plane, and rat body fixing components, The mouse tail positioning device and the mouse tail clamping assembly, the mouse tail positioning device includes a channel for the mouse tail to pass through; it also includes a platform on an inclined surface, the upper surface of the platform is parallel to the horizontal plane, and the bearing There is a socket for placing the EP tube on the surface of the platform. The invention provides a mouse tail vein blood collection system to solve the problems in the prior art that it is difficult to ensure the stability of the tail of the blood to be taken during the mouse tail vein blood collection process, and it is easy to mix air bubbles in the blood sample to interfere with the follow-up experiment. Rat tail vein blood sampling for the purpose of stability and continuity.

The invention relates to a near-infrared fluorescent probe molecule capable of being combined with thrombus emboli and application of the near-infrared fluorescent probe molecule in pulmonary embolism modeling and non-invasive quantitative detection. According to a method, rat plasma, thrombin, calcium chloride and near-infrared fluorescent probe molecules form traceable thrombus emboli in vitro, and the traceable thrombus emboli is cut into pieces, moderately ground to micron scale and then injected into a rat body through caudal vein. Thromboembolic particles can be accumulated in lung blood vessels of a rat to block the blood vessels to form embolism, construction of a rat pulmonary embolism model is achieved, non-invasive quantitative detection can be achieved with assistance of a small animal living body fluorescence imaging system, and the thromboembolic particles can be applied to evaluation of the thrombolysis effect of thrombolytic drugs and the like.

The invention discloses an oxtail vein hemostix. The oxtail vein hemostix comprises an oxtail fixing sleeve, wherein a side fixing shell is fixedly welded to one side of the outer wall of the oxtail fixing sleeve, a needle tube fixing assembly is movably mounted in the side fixing shell, and attaching moving assemblies are fixedly mounted in the outer wall of the oxtail fixing sleeve; a self-locking fixing assembly is movably mounted on the outer wall of the oxtail fixing sleeve and located outside the five attaching moving assemblies; a needle tube sleeve is further arranged in the needle tube fixing assembly; a belt is further arranged in each attaching moving assembly; strips are also arranged in the self-locking fixing assembly; and an oxtail mounting groove is fixedly formed in one side of the outer wall of the oxtail fixing sleeve. According to the oxtail vein hemostix, the hemostix can quickly, conveniently and fixedly sleeve the oxtail, an extra fixing operation is not needed,and the situation that the oxtail shakes, so that a screw scratches an ox body is avoided; and besides, in the blood sampling process, the mounting and the angle of the needle tube are fixed, the operation of an operator is facilitated, and the hands of the operator are released.

The invention discloses a preparation process of morchella polysaccharide and novel application thereof in prevention and treatment of thrombus and diseases caused by thrombus, and relates to the technical field of medicines and special medical foods. The morchella polysaccharide is obtained by extracting and purifying one or more of morchella, morchella deliciosa, morchella crassipes, double-rib morchella, morchella conica and congeneric morchella; and the diseases caused by thrombus comprise cerebral apoplexy, myocardial infarction, peripheral venous thrombosis, pulmonary embolism and the like. The prepared morchella polysaccharide can significantly inhibit carrageenan-induced mouse thrombus, mouse caudal vein thrombus formation and mouse liver and lung blood vessel thrombus formation, the morchella polysaccharide serving as an antithrombotic drug is extremely high in safety and high in medication compliance, large-scale artificial planting of morchella is achieved, and the source is wide, therefore, the morchella polysaccharide has a relatively wide application prospect in prevention and treatment of thrombus and diseases caused by thrombus.

The invention discloses the application of MicroRNA-302c-3p as an NLRP3 inhibitor; the invention screens and identifies miRNAs directly targeting NLRP3 through an online bioinformatics database, and screens out the most significant miR-302c through various experimental verifications ‑3p; miR‑302c‑3p inhibits endothelial inflammation and pyroptosis by directly binding to a specific site of NLRP3 mRNA; and after injecting miR‑302c‑3p agomir through the tail vein, aortic inflammation and pyroptosis in ApoE‑ / ‑ mice were both improved; studies have shown that miR-302c-3p / NLRP3 has a new regulatory signaling pathway and its mechanism of action in endothelial cells; MicroRNA-302c-3p can directly bind to specific sites of NLRP3 mRNA to inhibit NLPR activation, thereby inhibiting endothelial inflammation and pyroptosis can achieve the purpose of treating atherosclerosis.