13 results about "Multi photon microscopy" patented technology

A laser system for multiphoton microscopy comprises a seeder oscillator, fast modulator, amplifier, second modulator, and optical parametric amplifier configured to produce pulses with variable pulse spacing temporally synchronized to a scanner having sinusoidal motion. The variable pulse spacing ensures each pixel receives substantially the same number of laser pulses, eliminating uneven energy distribution of fixed repetition rate systems. The scanner operates at 4-12 kHz frequencies while the seeder oscillator produces femtosecond pulses at 10-100 MHz with 10-100 ns pulse spacing. The optical parametric amplifier outputs wavelengths from 600-1900 nm, with 1300-1700 nm optimized for three-photon microscopy. Methods include synchronizing pulse timing to scanner motion and varying interpulse intervals across scan lines. For 8 kHz scanners with 512 pixels per line, the system achieves 32% energy reduction compared to constant pulse spacing while maintaining 100-150 mW average power on biological samples, preventing thermal damage and enabling video rate imaging.

The invention discloses a rare earth homologous dual-emission high-resolution deep imaging method based on deep learning enhancement. A plurality of long-life intermediate states of lanthanide upconversion nanoparticles (UCNPs) are utilized, a single 980nm pumping source is used for exciting Tm < 3 + > / Yb < 3 + > co-doped UCNPs, and two-photon fluorescence with higher penetrability and four-photon fluorescence with higher resolution are induced at the same time. By combining a dual-mechanism CycleGAN artificial neural network based on antagonism training and cyclic consistency constraint, cross-domain mapping between two fluorescence signals is established, and the advantages of two fluorescence imaging are cooperatively utilized, so that deep penetration and high-resolution imaging are realized. According to the invention, in a self-established wide-field microscopic imaging system, the resolution of 209nm of a single particle in a 808nm fluorescence channel can be realized, and the signal-to-background ratio is doubled. According to the invention, the technical bottleneck that the resolution and the penetration depth of a traditional multi-photon microscope in deep tissue imaging are mutually restricted is effectively solved.

The invention provides a multi-mode imaging system fusing optical coherence tomography (OCT) and multi-photon microscopic imaging, which is characterized in that optical system design is carried out on the basis of a laser light source and a laser control module of an OCT shared multi-photon microscope, and various imaging modes such as OCT angiography, Doppler OCT, multi-photon fluorescence imaging, second harmonic and third harmonic are fused. Synchronous acquisition of multi-mode and multi-dimensional optical information is achieved, and the application range is wider.

The utility model relates to an integrated light path modulation device which comprises a shell, a modulation assembly and an electric light path switching assembly. The shell is provided with an input interface and an output interface; the modulation assembly comprises a shutter, a polarization adjustment module, a power adjustment beam splitting module and a switchable beam expanding part, the input interface is connected with the laser, and the shutter, the polarization adjustment module, the power adjustment beam splitting module and the light path switching part are sequentially installed; a light inlet of the beam expanding part is positioned on one side of the light path switching part, and an output interface is connected with the multi-photon microscope; the electric light path switching assembly is installed between the power adjusting beam splitting module and the light path switching part. Compared with the prior art, the device integrates the functions of shutter control, polarization adjustment, power adjustment, beam splitting and adjustable beam expansion, is compact and stable in structure, highly integrated and expandable, and meets the requirements of flexible regulation and control of different lasers.

The utility model discloses a multiphoton microscope with lens protection, which comprises a microscope seat, a microscope arm mounted on the microscope seat, an adjusting seat arranged at the top end of the microscope arm, a coarse focusing screw mounted on one side of the adjusting seat, a fine focusing screw mounted on the other side of the adjusting seat, a multiphoton lens barrel arranged on one side of the adjusting seat, and an eyepiece arranged at the top of the multiphoton lens barrel. An objective lens is installed at the bottom of the multi-photon lens cone, an objective table is arranged below the objective lens and arranged on one side of the lens arm, a moving mechanism is arranged on one side of the objective table, a lower dust shielding cover is arranged at one end of the moving mechanism, a plurality of dust shielding grooves are formed in the lower dust shielding cover and used for being matched with an objective lens, and a light chopper is installed at the bottom of the objective table. The bottom of the lens arm is also provided with a mounting rack, the mounting rack is internally provided with a light source generator, and the light source generator is used for illumination detection. The microscope has the advantages that the eyepiece and the objective lens are protected, the lens is prevented from being damaged, the overall structure is simple and convenient to disassemble and assemble, and the objective table is protected to a certain extent.

The invention provides improved systems and methods for multiphoton microscopy including pixel clocking techniques for minimizing pixel integration time and providing consistent signal intensity with maximized imaging speeds. Various systems and method are described for optimizing laser repetition rate based on dye lifetime, combining polygonal mirror scanning and stage translation, using the laser pulse signal to time pixel collection, and minimizing laser pulses and dye usage based on signal to background ratios.

The invention provides a miniaturized multi-photon microscope and an imaging method, and relates to the technical field of microscopes. The miniaturized multi-photon microscope comprises a multi-wavelength laser management module, a Tlens miniature zoom lens and a galvanometer, wherein the multi-wavelength laser management module comprises at least three independent femtosecond lasers; wherein the multi-wavelength laser management module is used for transmitting a plurality of laser pulses with different wavelengths through at least three independent femtosecond lasers, and transmitting a combined laser pulse formed by coupling the plurality of laser pulses with different wavelengths to the Tlens miniature zoom lens, and the wavelength of the laser pulse is located in a near-infrared second area range; the Tlens miniature zoom lens is used for changing the divergence angle of the combined laser pulse and outputting a target light beam with a controllable divergence angle; the galvanometer is used for reflecting and scanning a target light beam with a controllable divergence angle.

A microscope objective having proximal and distal ends comprises a plurality of lenses including a lens element closest to the proximal end, a lens element closest to said distal end and a plurality of lens elements therebetween. At least one of the lenses has a clear aperture of larger than 35 mm. The microscope objective has a working distance of between 8 mm and 14 mm and a numerical aperture of between 0.55 and 0.65 in air.

In one aspect, the disclosure relates to a method for correcting distortions or aberrations in a multi-photon microscopy image, the method including at least: (a) illuminating the sample; (b) imaging the illuminated sample with an imaging device having one or more detection elements that receive a signal from the sample; and (c) processing the image using a scattering correction and a phase correction. The phase correction can be a low-order deformable mirror adaptive optics correction with a hardware component and a software component. In some aspects, a spatial light modulator can be employed as a low-order phase correcting device. The scattering correction can be intensity scattering correction, phase scattering correction, or both, and can be performed with a high-order digital micromirror device with binary intensity modulation or a phase or intensity high-order spatial light modulator device. Also disclosed are methods of imaging complex tissue in living subjects.

The invention relates to a laser microscope (20), in particular multiphoton microscope, for the histological examination of a tissue sample (14), comprising: a laser light source (27), laser optics (21), a measurement signal acquisition module (28) and a sample receiver (30) for receiving the tissue sample (14) and / or a sample holder for a tissue sample (14), wherein: the laser light source (27) is designed to produce laser light (L) having an excitation wavelength of between 950 nm and 1200 nm, in particular between 1000 nm and 1100 nm, more particularly between 1030 nm and 1070 nm, preferably 1050 nm; and the measurement signal acquisition module (28) is arranged and designed to detect fluorescent light (XF) reflected by the tissue sample (14) into the laser optics (21), in particular having a fluorescence wavelength of between 500 nm and 700 nm, preferably between 550 nm and 650 nm, and / or ultraviolet THG light (XT), in particular having a THG wavelength of between 300 nm and 400 nm, in particular between 330 nm and 370 nm, in particular between 340 nm and 360 nm, preferably 350 nm. The invention is characterized in that the sample holder (30) is arranged above the laser optics (21) in such a way that laser light (L) emitted by the laser optics (21) is incident on the tissue sample (14) in particular only through the sample receiver (30), and in particular the sample receiver (30) is freely accessible on a side remote from the laser optics (21).

The invention provides improved systems and methods for multiphoton microscopy including pixel clocking techniques for minimizing pixel integration time and providing consistent signal intensity with maximized imaging speeds. Various systems and method are described for optimizing laser repetition rate based on dye lifetime, combining polygonal mirror scanning and stage translation, using the laser pulse signal to time pixel collection, and minimizing laser pulses and dye usage based on signal to background ratios.

The invention relates to the technical field of optical microscopic imaging, in particular to a wide-view-field multi-photon microscopic imaging method, device and equipment, and the method comprises the steps: obtaining training data collected by a multi-photon microscope according to a rolling sub-sampling scanning strategy, the strategy covers the entire field of view by successively and longitudinally moving a periodic scan path of high frame rate, low spatial sampling rate. Preprocessing such as normalization, time sequence registration or space division is carried out on the data, and a mask matrix is generated; and performing cross supervision by using the complementary region to construct a self-supervised training sample and a pseudo tag. And training a three-dimensional convolutional neural network based on the training samples and the pseudo labels, and performing reasoning reconstruction on the rolling sub-sampling data by using the trained network to obtain a target image sequence. Therefore, the problem that in the prior art, the high spatial sampling density causes the low frame rate, and the high frame rate needs the small imaging area or the low spatial sampling rate, that is, the imaging view field and the imaging temporal-spatial resolution are difficult to consider at the same time is solved.

The present disclosure provides an optical instrument, imaging system, and imaging method for use in a compact multiphoton microscope, and relates to an optical arrangement area. The optical instrument has a fluorescence excitation light path and a fluorescence collection light path, and includes a condenser lens, the condenser lens including a first lens group and a second lens group spaced apart from each other, the first lens group being located in the fluorescence collection light path, the second lens group being located in the fluorescence excitation light path and the fluorescence collection light path, and the second lens group being closely attached to a microscope objective lens. The solution disclosed herein improves the collection efficiency of the compact multiphoton microscope for scattered fluorescence and improves the imaging signal-to-noise ratio when observing a sample with scattering properties.