6 results about "Multi photon microscopy" patented technology

A laser system for multiphoton microscopy comprises a seeder oscillator, fast modulator, amplifier, second modulator, and optical parametric amplifier configured to produce pulses with variable pulse spacing temporally synchronized to a scanner having sinusoidal motion. The variable pulse spacing ensures each pixel receives substantially the same number of laser pulses, eliminating uneven energy distribution of fixed repetition rate systems. The scanner operates at 4-12 kHz frequencies while the seeder oscillator produces femtosecond pulses at 10-100 MHz with 10-100 ns pulse spacing. The optical parametric amplifier outputs wavelengths from 600-1900 nm, with 1300-1700 nm optimized for three-photon microscopy. Methods include synchronizing pulse timing to scanner motion and varying interpulse intervals across scan lines. For 8 kHz scanners with 512 pixels per line, the system achieves 32% energy reduction compared to constant pulse spacing while maintaining 100-150 mW average power on biological samples, preventing thermal damage and enabling video rate imaging.

The invention provides a multi-mode imaging system fusing optical coherence tomography (OCT) and multi-photon microscopic imaging, which is characterized in that optical system design is carried out on the basis of a laser light source and a laser control module of an OCT shared multi-photon microscope, and various imaging modes such as OCT angiography, Doppler OCT, multi-photon fluorescence imaging, second harmonic and third harmonic are fused. Synchronous acquisition of multi-mode and multi-dimensional optical information is achieved, and the application range is wider.

The utility model relates to an integrated light path modulation device which comprises a shell, a modulation assembly and an electric light path switching assembly. The shell is provided with an input interface and an output interface; the modulation assembly comprises a shutter, a polarization adjustment module, a power adjustment beam splitting module and a switchable beam expanding part, the input interface is connected with the laser, and the shutter, the polarization adjustment module, the power adjustment beam splitting module and the light path switching part are sequentially installed; a light inlet of the beam expanding part is positioned on one side of the light path switching part, and an output interface is connected with the multi-photon microscope; the electric light path switching assembly is installed between the power adjusting beam splitting module and the light path switching part. Compared with the prior art, the device integrates the functions of shutter control, polarization adjustment, power adjustment, beam splitting and adjustable beam expansion, is compact and stable in structure, highly integrated and expandable, and meets the requirements of flexible regulation and control of different lasers.

A microscope objective having proximal and distal ends comprises a plurality of lenses including a lens element closest to the proximal end, a lens element closest to said distal end and a plurality of lens elements therebetween. At least one of the lenses has a clear aperture of larger than 35 mm. The microscope objective has a working distance of between 8 mm and 14 mm and a numerical aperture of between 0.55 and 0.65 in air.

In one aspect, the disclosure relates to a method for correcting distortions or aberrations in a multi-photon microscopy image, the method including at least: (a) illuminating the sample; (b) imaging the illuminated sample with an imaging device having one or more detection elements that receive a signal from the sample; and (c) processing the image using a scattering correction and a phase correction. The phase correction can be a low-order deformable mirror adaptive optics correction with a hardware component and a software component. In some aspects, a spatial light modulator can be employed as a low-order phase correcting device. The scattering correction can be intensity scattering correction, phase scattering correction, or both, and can be performed with a high-order digital micromirror device with binary intensity modulation or a phase or intensity high-order spatial light modulator device. Also disclosed are methods of imaging complex tissue in living subjects.

The invention relates to the technical field of optical microscopic imaging, in particular to a wide-view-field multi-photon microscopic imaging method, device and equipment, and the method comprises the steps: obtaining training data collected by a multi-photon microscope according to a rolling sub-sampling scanning strategy, the strategy covers the entire field of view by successively and longitudinally moving a periodic scan path of high frame rate, low spatial sampling rate. Preprocessing such as normalization, time sequence registration or space division is carried out on the data, and a mask matrix is generated; and performing cross supervision by using the complementary region to construct a self-supervised training sample and a pseudo tag. And training a three-dimensional convolutional neural network based on the training samples and the pseudo labels, and performing reasoning reconstruction on the rolling sub-sampling data by using the trained network to obtain a target image sequence. Therefore, the problem that in the prior art, the high spatial sampling density causes the low frame rate, and the high frame rate needs the small imaging area or the low spatial sampling rate, that is, the imaging view field and the imaging temporal-spatial resolution are difficult to consider at the same time is solved.