47 results about "Multi photon microscopy" patented technology

Methods and devices are disclosed for acquiring depth resolved aberration information using principles of low coherence interferometry and perform coherence gated wavefront sensing (CG-WFS). The wavefront aberrations is collected using spectral domain low coherence interferometry (SD-LCI) or time domain low coherence interferometry (TD-LCI) principles. When using SD-LCI, chromatic aberrations can also be evaluated. Methods and devices are disclosed in using a wavefront corrector to compensate for the aberration information provided by CG-WFS, in a combined imaging system, that can use one or more channels from the class of (i) optical coherence tomography (OCT), (ii) scanning laser ophthalmoscopy, (iii) microscopy, such as confocal or phase microscopy, (iv) multiphoton microscopy, such as harmonic generation and multiphoton absorption. For some implementations, simultaneous and dynamic aberration measurements / correction with the imaging process is achieved. The methods and devices disclosed can provide wavefront sensing in the presence of stray reflections from optical interfaces.

According to one aspect of the invention, a wide-field microscope includes a stage configured to hold a specimen having a fluorescent material therein, and a multi-photon excitation light source configured to produce a substantially parallel beam of excitation light having a single photon energy less than an absorption energy required for single photon excitation of the fluorescent material. An infinity corrected objective is optically coupled to the multi-photon excitation light source and configured to focus the substantially parallel beam of excitation light onto the specimen such that multi-photon excitation of the fluorescent material simultaneously occurs over a predetermined area of the specimen. A focus lens is configured to focus emission light emitted from the predetermined area of the specimen onto at least two pixels of an image detector simultaneously. A focus lens is configured to focus emission light emitted from the predetermined area of the specimen onto an image plane, such that the image plane can be viewed through a binocular eyepiece or an imaging array detector.

The present invention is directed to a method of applying radiation through an optical fiber for detecting disease within a plant or animal or other penetrable tissue, or imaging a particular tissue of a plant or animal. In addition, fluorescence and nonlinear scattering signals can be detected and localized within a subject by such application of radiation through an optical fiber. The radiation is effective to promote simultaneous multiphoton excitation. The optical fibers are used alone to examine internal regions of tissue, in conjunction with an optical biopsy needle to evaluate sub-surface tissue, or with an endoscope to evaluate tissue within body cavities. The present invention also relates to a device for coupling in radiation from an ultrashort mode-locked laser into the beam path of a microscope.

An optical system and method are presented for use in a multi-photon microscope. The system comprises an imaging lens arrangement, and a pulse manipulator arrangement. The pulse manipulator arrangement comprises a temporal pulse manipulator unit which is accommodated in an optical path of an input pulse of an initial profile, and is configured to affect trajectories of light components of the input pulse impinging thereon so as to direct the light components towards an optical axis of the lens arrangement along different optical paths, said temporal light manipulator unit being accommodated in a front focal plane of the imaging lens arrangement, thereby enabling to restore the input pulse profile at an imaging plane.

Disclosed is an apparatus for division and rearrangement of light from a source object. The apparatus splits the light from the source object, or image of the source object, and recombines it in a parallel, fashion to increase the efficiency of multiphoton microscopy in general and harmonic or fluorescence emission microscopy in particular. The apparatus includes a beam splitter configured to split a light beam into at least two independent light paths to yield a first light path and a second light path; a first beam focuser configured to direct and focus the first light path onto a focal plane; and a second beam focuser configured to direct and focus the second light path onto the same or different focal plane to which the first light path is focused; and wherein the first and second light paths may be superimposed upon one another at a common focal plane or directed independently to different positions.

The invention belongs to the technical field of optical microscopic imaging, and particularly discloses a large-view-field microscope objective lens. The objective lens comprises a scanner and a plurality of lenses. A back focal plane calculated by the objective lens according to excitation light wavelength is positioned outside an objective lens body. When the scanner is a single single-axis scanner or a single double-axis scanner, the single single-axis scanner or the single double-axis scanner is positioned on the rear focal plane of the objective lens. When the scanners are two single-axisscanners in one group, the rear focal plane of the objective lens is positioned in the middle of the two single-axis scanners in one group. According to the invention, the design concepts that the diameter of an incident light beam is kept unchanged, the field angle is increased and the rear focal plane of the objective lens is externally arranged are adopted, and the high-numerical-aperture large-field imaging microscope objective lens with low cost and small size is provided for a standard desk type multi-photon microscope.

Light collectors for fluorescence microscopy include slab light guides having a specimen volume such as a specimen well configured to retain a specimen. The specimen volume is bounded by a surface of the slab light guide that permits laterally propagating fluorescence to enter the slab light guide and propagate toward a detection or imaging system. Typically, the slab light guide has an elliptical perimeter and the sample volume is situated at a first focus of the elliptical perimeter. A reflector such as a conical reflector is situated at the second focus so as to direct the collected fluorescence to exit the slab light guide.

An optical scanner, scanner apparatus, or scanner assembly, which may be particularly advantageous for use in a multiphoton microscope, includes a first drivable bending component, a second drivable bending component mounted perpendicularly to the first component, and at least one optical waveguide coupled one or both of the first and second bending components, wherein the at least one optical waveguide provides both a propagation path for a multiphoton excitation radiation delivery between a light source and a target and a multiphoton-induced emission radiation delivery between the target and a detector. A GRIN relay lens. A multiphoton microscope incorporating the scanner and the GRIN relay lens.

A dynamic focus and zoom system for use with wide-field, confocal and multiphoton microscopes having first, second and third MEMS mirrors situated within a housing. First and second fixed lenses form an optical relay. A prism / mirror is situated proximate to a first portal in the housing and is aligned linearly with the optical relay and a first MEMS mirror. The second MEMS mirror is proximate to and at a ninety-degree angle relative to the first MEMS mirror, which is at a forty-five-degree angle to the second fixed lens. The second MEMS mirror, third fixed lens and third MEMS mirror are aligned linearly, with the third fixed lens situated between the second and third MEMS mirrors. The third MEMS mirror is proximate to a second portal in the housing. The third MEMS mirror is situated adjacent to the prism / mirror.

The invention provides for compositions and process for fabricating an optical reflector constructed from biocompatible and bioresorbable silk fibroin proteins. For example, the silk retroreflectors may be built based on millimeter size microprism arrays to rotate the image plane of imaged cortical layers, thus enhancing the amount of photons that are detectable in the reflected direction when inserted in a sample to be analyzed, and ultimately increasing in contrast ratio in multiphoton microscopy. Such device can be used as a label-free, biocompatible, bioresorbable, implantable device for various applications ranging from medical imaging / diagnostics, drug / therapeutic delivery, to food chain safety and environmental monitoring.