47 results about "Multi photon microscopy" patented technology

The present invention is directed to a method of applying radiation through an optical fiber for detecting disease within a plant or animal or other penetrable tissue, or imaging a particular tissue of a plant or animal. In addition, fluorescence and nonlinear scattering signals can be detected and localized within a subject by such application of radiation through an optical fiber. The radiation is effective to promote simultaneous multiphoton excitation. The optical fibers are used alone to examine internal regions of tissue, in conjunction with an optical biopsy needle to evaluate sub-surface tissue, or with an endoscope to evaluate tissue within body cavities. The present invention also relates to a device for coupling in radiation from an ultrashort mode-locked laser into the beam path of a microscope.

Methods and devices are disclosed for acquiring depth resolved aberration information using principles of low coherence interferometry and perform coherence gated wavefront sensing (CG-WFS). The wavefront aberrations is collected using spectral domain low coherence interferometry (SD-LCI) or time domain low coherence interferometry (TD-LCI) principles. When using SD-LCI, chromatic aberrations can also be evaluated. Methods and devices are disclosed in using a wavefront corrector to compensate for the aberration information provided by CG-WFS, in a combined imaging system, that can use one or more channels from the class of (i) optical coherence tomography (OCT), (ii) scanning laser ophthalmoscopy, (iii) microscopy, such as confocal or phase microscopy, (iv) multiphoton microscopy, such as harmonic generation and multiphoton absorption. For some implementations, simultaneous and dynamic aberration measurements / correction with the imaging process is achieved. The methods and devices disclosed can provide wavefront sensing in the presence of stray reflections from optical interfaces.

According to one aspect of the invention, a wide-field microscope includes a stage configured to hold a specimen having a fluorescent material therein, and a multi-photon excitation light source configured to produce a substantially parallel beam of excitation light having a single photon energy less than an absorption energy required for single photon excitation of the fluorescent material. An infinity corrected objective is optically coupled to the multi-photon excitation light source and configured to focus the substantially parallel beam of excitation light onto the specimen such that multi-photon excitation of the fluorescent material simultaneously occurs over a predetermined area of the specimen. A focus lens is configured to focus emission light emitted from the predetermined area of the specimen onto at least two pixels of an image detector simultaneously. A focus lens is configured to focus emission light emitted from the predetermined area of the specimen onto an image plane, such that the image plane can be viewed through a binocular eyepiece or an imaging array detector.

The present invention is directed to a method of applying radiation through an optical fiber for detecting disease within a plant or animal or other penetrable tissue, or imaging a particular tissue of a plant or animal. In addition, fluorescence and nonlinear scattering signals can be detected and localized within a subject by such application of radiation through an optical fiber. The radiation is effective to promote simultaneous multiphoton excitation. The optical fibers are used alone to examine internal regions of tissue, in conjunction with an optical biopsy needle to evaluate sub-surface tissue, or with an endoscope to evaluate tissue within body cavities. The present invention also relates to a device for coupling in radiation from an ultrashort mode-locked laser into the beam path of a microscope.

An optical system and method are presented for use in a multi-photon microscope. The system comprises an imaging lens arrangement, and a pulse manipulator arrangement. The pulse manipulator arrangement comprises a temporal pulse manipulator unit which is accommodated in an optical path of an input pulse of an initial profile, and is configured to affect trajectories of light components of the input pulse impinging thereon so as to direct the light components towards an optical axis of the lens arrangement along different optical paths, said temporal light manipulator unit being accommodated in a front focal plane of the imaging lens arrangement, thereby enabling to restore the input pulse profile at an imaging plane.

Systems and methods herein provide improved, high-throughput multiphoton imaging of thick samples with reduced emission scattering. The systems and methods use structured illumination to modify the excitation light. A reconstruction process can be applied to the resulting images to recover image information free of scattering. The disclosed systems and methods provide high throughput, high signal-to-noise ratio, and high resolution images that are depth selective.

A two-photon imaging system capable of imaging of an image region in real time is presented. The imaging system comprises a source of excitation light that provides the excitation light as a plurality of laser beamlets. The plurality of laser beamlets is collectively scanned by a single-axis scanner along a first direction in the focal plane of the image region and oriented such that neither the rows nor columns are aligned with the first direction. As a result, each laser beamlet scans a different sub-region of the image region and the plurality of sub-regions are simultaneously scanned. As a result, the entirety of the image region is scanned in the same amount of time required to scan one image sub-region.

Disclosed is an apparatus for division and rearrangement of light from a source object. The apparatus splits the light from the source object, or image of the source object, and recombines it in a parallel, fashion to increase the efficiency of multiphoton microscopy in general and harmonic or fluorescence emission microscopy in particular. The apparatus includes a beam splitter configured to split a light beam into at least two independent light paths to yield a first light path and a second light path; a first beam focuser configured to direct and focus the first light path onto a focal plane; and a second beam focuser configured to direct and focus the second light path onto the same or different focal plane to which the first light path is focused; and wherein the first and second light paths may be superimposed upon one another at a common focal plane or directed independently to different positions.

The present invention is applicable to the field of optoelectronic technology, and provides a self-reference measuring device; the self-reference measuring device comprises an excitation unit, a firstoptical path adjusting unit, a soliton generating unit, a second optical path adjusting unit and a multiphoton microscope unit. The excitation unit is used for generating pump light with a preset wavelength and allowing the pump light to enter the first optical path adjusting unit; the first optical path adjusting unit is used for adjusting the polarization state of the pump light and allowing the pump light to enter the soliton generating unit, the the soliton generating unit is used to generate a laser pulse according to the pump light and allow the laser pulse to enter the second optical path adjusting unit; the second optical path adjusting unit is configured to process the laser pulse and allow the laser pulse to enter the multiphoton microscope unit, and the multiphoton microscope unit is used for measuring axial chromatic aberration based on imaging of a sample and transmitting harmonic signals to a signal collection unit for collection. The self-reference measuring device doesnot require a mechanical displacement platform to repeat scanning all the time, thereby eliminating mechanical errors introduced by the displacement platform when monochromatic excitation light repeatedly scanns.

The invention belongs to the technical field of optical microscopic imaging, and particularly discloses a large-view-field microscope objective lens. The objective lens comprises a scanner and a plurality of lenses. A back focal plane calculated by the objective lens according to excitation light wavelength is positioned outside an objective lens body. When the scanner is a single single-axis scanner or a single double-axis scanner, the single single-axis scanner or the single double-axis scanner is positioned on the rear focal plane of the objective lens. When the scanners are two single-axisscanners in one group, the rear focal plane of the objective lens is positioned in the middle of the two single-axis scanners in one group. According to the invention, the design concepts that the diameter of an incident light beam is kept unchanged, the field angle is increased and the rear focal plane of the objective lens is externally arranged are adopted, and the high-numerical-aperture large-field imaging microscope objective lens with low cost and small size is provided for a standard desk type multi-photon microscope.

Light collectors for fluorescence microscopy include slab light guides having a specimen volume such as a specimen well configured to retain a specimen. The specimen volume is bounded by a surface of the slab light guide that permits laterally propagating fluorescence to enter the slab light guide and propagate toward a detection or imaging system. Typically, the slab light guide has an elliptical perimeter and the sample volume is situated at a first focus of the elliptical perimeter. A reflector such as a conical reflector is situated at the second focus so as to direct the collected fluorescence to exit the slab light guide.

An optical scanner, scanner apparatus, or scanner assembly, which may be particularly advantageous for use in a multiphoton microscope, includes a first drivable bending component, a second drivable bending component mounted perpendicularly to the first component, and at least one optical waveguide coupled one or both of the first and second bending components, wherein the at least one optical waveguide provides both a propagation path for a multiphoton excitation radiation delivery between a light source and a target and a multiphoton-induced emission radiation delivery between the target and a detector. A GRIN relay lens. A multiphoton microscope incorporating the scanner and the GRIN relay lens.

A multi-photon microscope has an illumination source, an objective lens unit arranged in an optical path of the illumination source, a first light collection system arranged to collect a first portion of light emitted from a sample when the sample is illuminated by light from the illumination source, and a second light collection system arranged to collect a second portion of light emitted from the sample when the sample is illuminated by light from the illumination source. The first portion of light when collected by the first light collection system and the second portion of light when collected by the second light collection system, together provide a means of collecting as much light from as many angles as possible emanating from an emitting point source. This collection scheme has the potential to approach the total emission collection of light from an emitting point source depending on the optical properties of the sample being imaged.

A dynamic focus and zoom system for use with wide-field, confocal and multiphoton microscopes having first, second and third MEMS mirrors situated within a housing. First and second fixed lenses form an optical relay. A prism / mirror is situated proximate to a first portal in the housing and is aligned linearly with the optical relay and a first MEMS mirror. The second MEMS mirror is proximate to and at a ninety-degree angle relative to the first MEMS mirror, which is at a forty-five-degree angle to the second fixed lens. The second MEMS mirror, third fixed lens and third MEMS mirror are aligned linearly, with the third fixed lens situated between the second and third MEMS mirrors. The third MEMS mirror is proximate to a second portal in the housing. The third MEMS mirror is situated adjacent to the prism / mirror.

The invention provides for compositions and process for fabricating an optical reflector constructed from biocompatible and bioresorbable silk fibroin proteins. For example, the silk retroreflectors may be built based on millimeter size microprism arrays to rotate the image plane of imaged cortical layers, thus enhancing the amount of photons that are detectable in the reflected direction when inserted in a sample to be analyzed, and ultimately increasing in contrast ratio in multiphoton microscopy. Such device can be used as a label-free, biocompatible, bioresorbable, implantable device for various applications ranging from medical imaging / diagnostics, drug / therapeutic delivery, to food chain safety and environmental monitoring.