The invention relates to a single-
wavelength two-
photon STED and dual-
wavelength single-
photon STED coupled imaging device comprising a
femtosecond laser device, a
picosecond laser device, an I electric
shutter, an I
half wave plate, an I optical splitter, an II optical splitter or an I reflector switching device, an II
half wave plate, an I quarter wave plate, an II electric
shutter, an II reflector, an III reflector, an I
relay reflector group, an IV reflector, an I dichroic mirror, a V reflector, an II
relay reflector group, a VI reflector, a glass rod, an III
half wave plate, a
polarizer,a VII reflector, an I lens, a
polarization maintaining optical fiber, an II lens, a VIII reflector, a
phase plate, an IX reflector, an enhancing
optical path reflector group and a
confocal scanning
microscope. The imaging device provided by the invention can be switched to the single-
wavelength two-
photon STED imaging
system and the dual-wavelength single-photon imaging
system in a time-sharing manner according to needs.