The present invention discloses a device for measuring the width of the crystallization metastable region of a micro-scale flow system. The device comprises a temperature control system, a microscope,a camera, a computer and an injection pump, and the temperature control system is composed of a constant-temperature circulating water bath device, a first heat preservation jacket, a second heat preservation jacket and a temperature sensor. According to a method for measuring the width of the crystallization metastable region of the micro-scale flow system, by using the above device, a measuredsolution with the temperature being the initial test temperature and reaching the saturation state at the initial test temperature is injected into a micro-channel at the constant flow rate through the injection pump, and by combining the amplification effect of the microscope on the micro-channel, the camera is adopted to shoot the measured liquid in the micro-channel in real time, the crystallization state of the liquid is captured, the crystallization point temperature is obtained, the difference value between the initial test temperature and the crystallization point temperature is calculated, and the difference value is the width of the crystallization metastable region of the micro-scale flow system of the measured solution. The device and the method provided by the invention providethe quantitative data analysis support for the micro-channel-crystallization coupling technology research.