The invention relates to an operation specification for cleaning and disinfecting an air-conditioner cooling tower. The operation specification is characterized by including the following steps that 1, field investigation and preparation are performed, specifically, field investigation of normal running states of the cooling tower is performed, the actual situation is analyzed, and then an individualized operation scheme is worked out; an investigator needs to prepare personal protection very well, like wearing a mask and a pair of goggles; and key points of field investigation lie in that (1) dirt, algae and corrosion degrees of a water distributor, a filler, a tower tray (water collecting tray) and a backwater filtering net are investigated, (2) the investigator needs to know the situation of water replenishment of the cooling tower, (3) whether a water replenishing valve and a sewage discharge valve are intact or leak water or not is checked, (4) the investigator needs to know the corrosion degree of a support of the cooling tower, (5) whether an exhaust outlet is aligned to the cooling tower or not is checked, for oily fume increases the cleaning difficulty, and (6) an investigation report or advices are written, a plan is worked out, and suitable cleaning and disinfection agents, tools and equipment are prepared.