The invention belongs to the technical field of packaging materials, and particularly relates to a biomass honeycomb paperboard, and a preparation method and application thereof. The biomass honeycombpaperboard comprises wheat straw, rice husks, cotton seed hulls, corncobs, wheat bran, corn flour, quick lime and gypsum, or comprises corn straw, the wheat straw, the wheat bran, the corn flour, thequick lime and the gypsum, or comprises the corncobs, the cotton seed hulls, the wheat bran, the quick lime and the gypsum. The biomass honeycomb paperboard has the advantages that use of conventional paper is reduced, and damage of papermaking to the environment is lowered; excessive crop waste is utilized, and a crop waste treatment method is provided; material density is reduced through the honeycomb structure, production cost is saved, and transport cost is reduced; the biomass honeycomb paperboard is formed integrally, has lower production and transport costs than honeycomb paperboards,and has broad market prospect and high economic value.