The invention relates to a biological stuffing for denitrification. The biological stuffing for denitrification comprises a spherical shell, wherein a plurality of meshes are formed in the surface of the shell, the shell is filled with a biological carrier stuffing, and the biological carrier stuffing is formed by combining two types of granular substance made from different materials. One granular substance is cuboid massive particles made from a nano modified polyurethane material, the other granular substance is porous mineral particles, and the filling rate of the biological carrier filler in the shell is 35%-85%. The biological stuffing for denitrification has the advantages that the biological stuffing for denitrification is large in specific surface area, high in porosity, high biological adhesion, high in biofilm formation speed, high in anti-load capacity, high in denitrification efficiency, small in biological sludge yield, good in chemical and biological stability and durable, enables biofilm formation to be easy, is resistant to aging, acid and alkali, can be arranged directly without fixing and is convenient to install, no harmful substance is not dissolved, and secondary pollution is not caused.