37results about How to "Avoid depolymerization" patented technology

Methods of identifying subjects at increased risk of cancer, based upon detection of biofilms and / or biofilm-associated microbes within a subject, are disclosed. Therapies designed to prevent formation and / or reduce the size of biofilms in a subject identified to be at increased risk of cancer based upon detection of biofilms and / or biofilm-associated microbes are disclosed. In particular embodiments, the invention provides for identification of a subject at elevated risk of developing or having colorectal cancer and / or a colorectal adenoma, based upon detection of a biofilm and / or biofilm-associated bacteria within the gastrointestinal tract of the subject (optionally, within a biopsy specimen and / or stool sample of such subject). Therapies involving administration of an antibiotic agent and / or a probiotic agent to a subject, to prevent or reduce biofilm formation within the gastrointestinal tract of the subject, optionally provided in combination with additional cancer therapy, are also disclosed.

Cancer therapies that combine epigenetic modulating agent(s) with immune modulating agent(s), which were remarkably identified to provide an improved treatment regimen over single agent therapy, are disclosed. In particular embodiments, the invention provides for improved treatment of NSCLC in patients via administration of exemplary immune modulating agents anti-PD-1 antibody or anti-PD-L1 antibody, which were observed to show enhanced activity in combination with the exemplary epigenetic modulating agent 5-deoxyazacytidine. Further, expression markers of responsive neoplastic cells are also disclosed.

The invention is concerned with a kind of rubber disintegrator. There are two roller canisters set in type of revolving on axis seat and they move inside of the roller. The axis of roller canister is fixed on frame of machine through the bearing seat, and the motor and reducer is droved by the revolving on axis seat and they move inside of the roller. The axis of roller canister is fixed on frame of mach axis of the two roller canisters. The said roller canister is made up of some slice type of drills on the axis with lengthways congruence and the drills of two roller canisters are interleaving. The roller canisters move to different directions and send the top of rubber block between the two roller canisters into the drills for cutting. The structure to exit of stuff is outer screen; with exit of stuff and the main part wrapped by the outer screen is the main body of the two roller canisters. The outer screen is a column and the side of it is the screen, the two bottom of it has circumferential rim and the bottom of each rim on the revolving axis seat is driven by transmission framework.

Production of polyalkylene carbonates via polymerization of carbon dioxide with at least one epoxide of the general formula (I):where R re mutually independently H, halogen, NO2, CN, COOR′ or C1-20-hydrocarbon moiety, which can have substitution, where one of the moieties R can also be OH, and where two moieties R can together form a C3-5-alkylene moiety, R′ is H or C1-20-hydrocarbon moiety, which can have substitution; n a zinc salt of C4-8-alkanedicarboxylic acids as catalysts, where a carboxylic acid or an acidic ion exchanger and a non-water-miscible organic solvent which dissolves the polyalkylene carbonate are admixed with the reaction mixture obtained after the reaction, and the organic phase is washed with water, and the polyalkylene carbonate is optionally obtained from the organic phase.

The invention discloses a preparation method of a hydrophobic charge induction chromatography medium with large capacity, and belongs to the protein chromatography separation technology in the field of biochemical engineering. The chromatography medium refers to the end of a space arm that is coupled with imidazol compounds as a chromatography matrix. The preparation method is that: the medium is activated after dimethyl sulfoxide, epichlorohydrin and sodium hydroxide solution are sequentially put into agarose gel medium; the activated medium is coupled with the imidazol compounds matrix in the alkaline dimethyl sulfoxide solution; finally, the epoxy left on the medium surface gets the needed chromatography medium after being reduced. The adsorption capacity of the chromatography medium to the protein achieves a moist medium of 80mg / ml and keeps relatively stable within a broader ionic strength scope from 0.1 mol / L to 1.0 mol / L; at the same time, the protein elutropic model which is finished by depending on the regulation of PH between 4.0 and 6.0 is more moderate, thereby having the wide application prospect in the separation and purification of biomacromolecule such as the protein, etc.

The invention discloses a method for rapidly preparing high-yield double-electricity chitin nanofiber. The method comprises the steps of firstly utilizing concentrated alkaline for carrying out deacetylation on chitin, endowing the chitin with amidogen, and then utilizing a TEMPO-NaClO2-NaClO system for oxidizing, thus obtaining the chitin nanofiber with amidogen and carboxyl at the same time, so that the prepared chitin nanofiber has double-electricity, not only can be dispersed under an acid condition, but also can be dispersed under an alkaline condition, and is favorable for surface modification and design by rich functional groups on the surface; the preparation yield is higher, and large-scale production is facilitated, so that the application range of the chitin nanofiber is expanded.

The invention discloses immobilized maleic acid cis-trans isomerase and a preparation method and application thereof, and belongs to the technical fields of enzymology and enzyme engineering and the field of biochemical industries. According to the immobilized maleic acid cis-trans isomerase and the preparation method and application thereof, R5 peptide fragments are connected to the N ends of the maleic acid cis-trans isomerase, R5-MaiA is crosslinked through glutaraldehyde, embedding is conducted on the crosslinked R5-MaiA by utilizing silicon reaction liquid, and immobilization of the maleic acid cis-trans isomerase is completed. The immobilized maleic acid cis-trans isomerase (R5-MI-CLEA-Si) with high stability is obtained through the method which is low in cost and easy to operate, the optimum reaction temperature of the immobilized isomerase is 55 DEG C, and the optimum reaction pH is 7.7; on the condition that the temperature is 55 DEG C, the half-life period of the immobilized isomerase is 4 h, the half-life period of the free isomerase is 0.5 h, the stability of the immobilized isomerase is eight times of that of the free isomerase, and after a catalytic reaction is repeated for eight times, the enzyme activity of the immobilized isomerase can be still retained at 75%.

The invention is concerned with a kind of rubber disintegrator. There are two roller canisters set in type of revolving on axis seat and they move inside of the roller. The axis of roller canister is fixed on frame of machine through the bearing seat, and the motor and reducer is droved by the revolving on axis seat and they move inside of the roller. The axis of roller canister is fixed on frame of mach axis of the two roller canisters. The said roller canister is made up of some slice type of drills on the axis with lengthways congruence and the drills of two roller canisters are interleaving. The roller canisters move to different directions and send the top of rubber block between the two roller canisters into the drills for cutting. The structure to exit of stuff is outer screen; with exit of stuff and the main part wrapped by the outer screen is the main body of the two roller canisters. The outer screen is a column and the side of it is the screen, the two bottom of it has circumferential rim and the bottom of each rim on the revolving axis seat is driven by transmission framework.

The present invention relates to a polylactide resin having excellent heat resistance, a preparation method thereof, and a polylactide resin composition including the same.The polylactide resin is characterized by high polymerization activity owing to an organic coordinated metal catalyst even though it has a low catalyst content, and a molecular weight reduction due to thermal decomposition at high temperature and a rate of thermal decomposition including depolymerization are greatly suppressed by a low catalyst content and a low resin acidity, and thus provided is the polylactide resin having superior heat resistance.

The invention provides a preparation method of high-performance polysulfone resin. The preparation method includes adding 45-55 parts of 4,4-dichlorodiphenylsulfone, 35-40 parts of salt-forming agent, 80-100 parts of low boiling point organic solvent and 0.01-0.04 parts of 3-fluoro-4-chlorobenzophenone into the reactor, and then 20-60 parts of 4,4'- -dihydroxydiphenylsulfone or bisphenol, injecting nitrogen gas and heating until the temperature inside the reactor rises to 200-210 DEG C, maintaining reaction at constant temperature for 1 to 2 hours, and after the temperature rises to 240-260 DEG C for 4 to 7 hours, adding 0.09-0.16 parts of 3-fluoro-4-chloro benzophenone, reacting 10-20 minutes, then stopping reactor and treating the reactant to obtain high-performance polysulfone resin. The polysulfone resin prepared by the method has high molecular weight, good transparency and heat resistance.

Methods for use of a multi-arm polyethylene glycol (PEG) modifier in modification of asparaginase. The described multi-arm PEG modifier enhances the subunit interaction of a multimeric protein to maintain the multimeric protein in a polymerized form, thereby improving the stability of the multimeric protein, maintaining the bioactivity of the multimeric protein, and reducing the probability of exposure of the antigen binding site after depolymerization of the subunits, so as to reduce the immunogenicity.

The invention relates to a method for manufacturing polylactide, comprising the steps of mixing lactide and a metal-coordination compound as polymerization catalyst to obtain a reaction mixture, polymerizing the lactide in liquid phase at a temperature of at least 150° C. to form polylactide in liquid phase and allowing the polylactide to solidify, characterized in that the polymerization catalyst comprises a metal-ligand coordination compound whereby the parent ligand answers the formula (I), whereby R represents an H atom, an aliphatic group, a halide atom or a nitro group and the metal is at least one of Zr and Hf. The invented catalysts show kinetics which is comparable to the kinetics of the known Sn-octoate catalyst.

The invention discloses a preparation method of 3-carboxymethyl-1,2,4-cyclopentanetricarboxylic acid-1,4:2,3-dianhydride and belongs to the field of preparation methods of fine chemicals. The main technical scheme is that dicyclopentadiene is taken as a starting material and subjected to oxidation reaction with ozone and hydrogen peroxide sequentially, intermediate product 2,3,5-tricarboxylic acidcyclopentane acetic acid is obtained, finally, acetic anhydride is taken as a dehydrating agent, and dehydration ring closing is performed for synthesizing the target product 3-carboxymethyl-1,2,4-cyclopentanetricarboxylic acid-1,4:2,3-dianhydride. The method in the scheme has the advantages of being mild in reaction condition, safe to operate, high in product purity and yield, pollution-free andthe like, and is suitable for large-scale industrial production.

The invention relates to injectable hydrogel with antibacterial, antioxidant and hemostatic effects and a preparation method and application thereof, the preparation method comprises the following steps: mixing an oxidized hyaluronic acid solution and a tannic acid solution, then mixing with a quaternized chitosan solution, and carrying out Schiff base reaction polymerization to obtain the injectable hydrogel. The hydrogel overcomes the defects that hyaluronic acid chitosan hydrogel in the prior art is poor in mechanical strength, rapid in degradation and the like; gel is formed in situ through Schiff base reaction, a cross-linking agent is not needed, and the method is green and safe; the device can be used for injection at a wound part, and has strong adaptability to wounds with irregular shapes; and the gel is degradable, so that a secondary operation is avoided. The polyphenol free radical scavenging activity of the tannic acid can effectively inhibit wound secondary oxidative stress injury caused by ROS (reactive oxygen species) and is beneficial to wound antibiosis and inflammation diminishing, meanwhile, the tannic acid can coagulate damaged collagen and provide an effective in-vivo hemostatic effect, and more importantly, after the tannic acid is introduced, the mechanical strength of the gel can be further enhanced.

The invention discloses a method for carrying out ICSI (intracytoplasmic sperm injection) on a pig. The method for carrying out ICSI on the pig comprises the following steps: (1) hydrolyzing sperms in vitro animal semen with lipoidase or lipoidase solution, and collecting a hydrolysis product, so that the sperms used for preparing ICSI fertilized ova; (2) injecting the sperms used for preparing the ICSI fertilized ova into in vitro oocytes to obtain ICSI ova; and (3) carrying out electric activation on the ICSI ova and cultivating, so that the ICSI fertilized ova are obtained. The method for carrying out ICSI on the pig has the advantages that bad effects on depolymerization of pig sperms, formation of male pronucleus and later development due to addition of PVP can be avoided, and regions such as plasma membranes and perforatorium of the pig sperms can not be damaged, so that release of oocyte activation promoting factors from the pig subjected to ICSI operation can be facilitated, and the formation rate of the male pronucleus and the formation rate of blastocysts of embryos after fertilization are increased.

The invention relates to the technical field of slow-release nitrogen fertilizer production. The invention further discloses a process method for preparing the agricultural slow-release nitrogen fertilizer by crushing and regenerating the waste amino resin. The preparation method comprises the following steps: preparing choline chloride and urea into eutectic ionic liquid, activating and degradingthe crushed powdery waste amino resin and processing the amino resin into amino resin slow-release nitrogen fertilizer liquid, and granulating and drying the nitrogen fertilizer liquid to finally obtain the amino resin slow-release nitrogen fertilizer. The initial nitrogen release rate of the slow-release nitrogen fertilizer is 1.5-2 times of the nitrogen release rate of common urea formaldehyde,the effective slow-release total amount is about 2-2.5 times of that of common urea formaldehyde, the effective release time is prolonged by 3-4 weeks, and the slow-release nitrogen fertilizer has agood slow-release effect.

The invention discloses a lipoic acid-silver nano self-healing material and its preparation method and application, and belongs to the field of self-healing materials. The lipoic acid-silver nano self-healing material prepared by the invention uses lipoic acid as the main raw material, and uses its strong reducing property to directly reduce silver ions into silver nanometers. The generated silver nanoparticles combine with the sulfur atoms of the end chain to prevent its depolymerization and ring closure, providing more active sites. In addition, dynamic disulfide bonds, coordination between silver ions and carboxyl groups, and hydrogen bonding all make lipoic acid-silver nano self-healing materials have strong mechanical strength and self-healing ability. The production of silver nanometers may provide the self-healing material with better antibacterial properties, catalytic properties and photothermal properties, and has certain application potential in medicine, environment and energy.

The invention discloses a new application of a multi-arm polyethylene glycol modifier and its application in modifying asparaginase. The multi-arm PEG modifier provided by the present invention can be used to strengthen the inter-subunit interaction force of multi-subunit proteins, keep multi-subunit proteins in the form of aggregation, thereby increasing the stability of multi-subunit proteins, enhancing or maintaining multi-subunit proteins biological activity, while reducing the chance of exposure of antigenic sites after depolymerization of each subunit, reducing immunogenicity. In addition, compared with the commercially available PEGylated asparaginase product "pegaspargase", the PEG-modified asparaginase prepared in the present invention has low immunogenicity, high activity and high stability, and PEG does not It has the advantages of falling off and the like, and is more suitable for the preparation of antitumor drugs.

The invention discloses a diketone derivative, as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The derivative comprises compounds in the general formula I and pharmaceutically-acceptable salt, wherein X is C or N; Y is C or N; R1 is one of hydrogen, chlorine, bromine or thiophene; R2 is one of hydrogen, isopropyl or methoxy. The most of compounds of the diketone derivative not only has an inhibiting effect on tau protein polymerization, but also plays a role in stabilizing microtubulin; the diketone derivative can be effectively used for treating the Alzheimer's disease.

The invention discloses immobilized maleic acid cis-trans isomerase and a preparation method and application thereof, and belongs to the technical fields of enzymology and enzyme engineering and the field of biochemical industries. According to the immobilized maleic acid cis-trans isomerase and the preparation method and application thereof, R5 peptide fragments are connected to the N ends of the maleic acid cis-trans isomerase, R5-MaiA is crosslinked through glutaraldehyde, embedding is conducted on the crosslinked R5-MaiA by utilizing silicon reaction liquid, and immobilization of the maleic acid cis-trans isomerase is completed. The immobilized maleic acid cis-trans isomerase (R5-MI-CLEA-Si) with high stability is obtained through the method which is low in cost and easy to operate, the optimum reaction temperature of the immobilized isomerase is 55 DEG C, and the optimum reaction pH is 7.7; on the condition that the temperature is 55 DEG C, the half-life period of the immobilized isomerase is 4 h, the half-life period of the free isomerase is 0.5 h, the stability of the immobilized isomerase is eight times of that of the free isomerase, and after a catalytic reaction is repeated for eight times, the enzyme activity of the immobilized isomerase can be still retained at 75%.

Disclosed herein is a metal enolate initiator for polymerizing isocyanates and a method for polymerizing isocyanates by anionic polymerization using the same, in which the initiator forms a cluster upon the initiation and protects stability of terminal anions at the end of the chain to cause controlled polymerization, thus preventing depolymerizaton and improving reaction time and efficiency without the use of a separate additive.

The invention discloses a diketone derivative, as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The derivative comprises compounds in the general formula I and pharmaceutically-acceptable salt, wherein X is C or N; Y is C or N; R1 is one of hydrogen, chlorine, bromine or thiophene; R2 is one of hydrogen, isopropyl or methoxy. The most of compounds of the diketone derivative not only has an inhibiting effect on tau protein polymerization, but also plays a role in stabilizing microtubulin; the diketone derivative can be effectively used for treating the Alzheimer's disease.

The invention relates to the use of CXCL4L1 as a biomarker of pancreatic cancer in a patient. More particularly, the invention relates to a method for detecting a pancreatic cancer and / or pancreatic metastasis in a patient, said method comprising determining the expression level of the CXCL4L1 gene in a biological sample obtained from said patient.

The invention provides a preparation method of high-performance polysulfone resin. The preparation method includes adding 45-55 parts of 4,4-dichlorodiphenylsulfone, 35-40 parts of salt-forming agent, 80-100 parts of low boiling point organic solvent and 0.01-0.04 parts of 3-fluoro-4-chlorobenzophenone into the reactor, and then 20-60 parts of 4,4'- -dihydroxydiphenylsulfone or bisphenol, injecting nitrogen gas and heating until the temperature inside the reactor rises to 200-210 DEG C, maintaining reaction at constant temperature for 1 to 2 hours, and after the temperature rises to 240-260 DEG C for 4 to 7 hours, adding 0.09-0.16 parts of 3-fluoro-4-chloro benzophenone, reacting 10-20 minutes, then stopping reactor and treating the reactant to obtain high-performance polysulfone resin. The polysulfone resin prepared by the method has high molecular weight, good transparency and heat resistance.

The invention discloses a preparation method of a compound protein surimi product, and belongs to the technical field of meat food processing. The preparation method comprises the steps of fish cream preparation, surimi preparation, core material preparation, core-wrapped surimi preparation and shell layer preparation. The preparation method has the advantages that a trypsin agent is added into surimi, so that denaturation of myofibrillar protein under the freezing condition can be inhibited, and in the curing process, cross-linking with whey protein and myofibrillar protein can be realized, so that the elasticity of the surimi is improved; the core material is coated with the surimi, so that the edible pleasure of the surimi product can be greatly improved, and the cheese, the yolk liquid, the maltodextrin and the cured soybean paste are blended, so that the dissolution of fat-soluble substances can be effectively avoided; and the slurry is molded and cured into a film on the surface of the surimi, so that a good water retaining effect on the surimi is achieved, and the retention rate of fat-soluble substances in the core material can be well realized.

Belonging to the technical field of biomedicine, the invention discloses a 4-oxo-2-thiothiazolidine containing derivative, a preparation method and application thereof. The derivative involved in the invention contains a compound shown as general formula I, wherein n is an integer 1 or 2; R1 is an ester group or amide or acid group; R2 is one of hydrogen, isopropyl or methoxy. Most compounds of the type have an inhibiting effect on tau protein polymerization, also has a stabilizing effect on tubulin, and can be effectively used for treatment of Alzheimer's disease.

Methods of identifying subjects at increased risk of cancer, based upon detection of biofilms and / or biofilm-associated microbes within a subject, are disclosed. Therapies designed to prevent formation and / or reduce the size of biofilms in a subject identified to be at increased risk of cancer based upon detection of biofilms and / or biofilm-associated microbes are disclosed. In particular embodiments, the invention provides for identification of a subject at elevated risk of developing or having colorectal cancer and / or a colorectal adenoma, based upon detection of a biofilm and / or biofilm-associated bacteria within the gastrointestinal tract of the subject (optionally, within a biopsy specimen and / or stool sample of such subject). Therapies involving administration of an antibiotic agent and / or a probiotic agent to a subject, to prevent or reduce biofilm formation within the gastrointestinal tract of the subject, optionally provided in combination with additional cancer therapy, are also disclosed.