The invention discloses a handheld medical cold-sealed air plasma jet spray gun in the field of plasma sterilization and disinfection, which comprises a device shell, and a discharge electrode, a Tesla coil, a touch screen, a cooling fan, an internal circuit, a control switch and a power lithium battery which are arranged in the device shell, wherein the power lithium battery is connected with the touch screen, the control switch and the Tesla coil through the internal circuit. The touch screen can regulate and control the pulse width of a square wave pulse signal and control the conduction time of an IGBT, on the one hand, tip pulse discharge is controlled, and the discharge jet flow temperature is reduced; on the other hand, the cooling fan is driven to be linked, when the pulse width is widened, the IGBT conduction time is prolonged, the rotating speed of the cooling fan is increased, the wind speed is increased, and the purpose of reducing the discharge jet flow temperature is achieved. Stable, continuous and high-density cold plasma is generated on the discharge electrode. The handheld medical cold-sealed air plasma jet spray gun has the advantages of high sterilization efficiency, universality and flexibility, can meet the sterilization requirement in the medical field, and is smaller in equipment size, convenient to carry and simple to operate.