The invention relates to a pharmaceutical preparation for treating gynecological vulvae itching and application of the pharmaceutical preparation. The pharmaceutical preparation comprises the following raw materials: fructus cnidii, potentilla disclor bunge, radix stemonae, radix sophorae flavescentis, cortex phellodendri, chrysanthemum indicum, radix glycyrrhizae, honeysuckle, yam, semen plantaginis, rhizoma atractylodis, radix bupleuri, pericarpium citri reticulatae, folium artemisiae argyi, cortex dictamni, sodium bisulfite, ethylenediamine tetraacetic acid disodium, carbomer, mint and glycerol. The adopted herbs of the pharmaceutical preparation have efficiencies of clearing heat and promoting dieresis, and are combined with auxiliary herbs capable of relieving itching and regulating as well as a matrix such as a ventilated ointment and a stabilizer, so that the external part of a vagina can keep dry, meanwhile, during treatment, normal flora in a patient body can be regulated effectively to improve the immune capacity of the patient, and the pharmaceutical preparation is good in treatment effect, low in cost, free of side effect, convenient to use, and suitable for being used by women of all ages suffering from vulvae itching.