The invention provides a tile grout composition and a preparation method and an application thereof, and belongs to the technical field of building materials. The tile grout composition can solve the problems of waste in time and labor, low construction efficiency, and difficulty in dense filling during the existing ground joint filling. The tile grout composition comprises cement, an aggregate, a filler, an expansion agent, a water reducing agent, a water retaining agent, emulsion powder, an early strength agent, a moisture repellent, a defoaming agent, a thixotropic agent and other raw materials; after the tile grout composition is mixed with water, the joint is filled, so that the leveling of the tile grout is good, a miniature gap can be fully filled, and the self-leveling is realized. The tile grout composition has the advantages that the density after hydration is good, the shrinkage rate is low, the strength is high, the joint filling construction of ground tiles is convenient, the early strength after construction is higher, the adhering strength after hardening is higher, and the cracking and water leakage after joint filling are avoided; the preparation method is simple, and the tile grout composition is suitable for industrial production, and is especially suitable for filling ground tile joints in building material industry.