The invention discloses a packaging method for rapidly obtaining high-titer lentivirus, which comprises the following steps: mixing lentivirus packaging plasmids, a target gene expression plasmid, a transfection reagent and protamine to form liposome, wherein the lentivirus packaging plasmids are pRsv-REV, pMDLG-pRRE and pVSV-G; adding the formed liposome into tool cells for transfection and culture; and collecting the cultured virus supernatant, and treating to obtain a virus solution. Through the mode, the optimized and improved formula of the four-plasmid system of the lentivirus packaging system and the transfection reagent are adopted, the virus packaging period can be greatly shortened, the time cost is saved, the method does not need to repeatedly replace medium for cells, the packaging steps are simple and easy to operate, the types of reagents used in the method are reduced, the operation is easy, and the lentivirus packaged by the method is high in titer and high in purity, and can be used for animal-level research.