The invention discloses a TPU gluing silicone rubber heat transfer film. The film sequentially comprises a silicone rubber bottom layer, a silicone rubber bonding layer and a TPU thin film; the silicone rubber bonding layer comprises, by mass part, 100 to 120 parts of vinyl silicone rubber based adhesive, 30 to 50 parts of vinyl silicone oil, 3 to 5 parts of polymethylhydrosiloxane, 0.02 to 0.05 part of an inhibitor, 0.05 to 0.2 part of a platinum catalyst, 3 to 5 parts of solid viscosifying powder, 3 to 5 parts of liquid silane coupling agent and 1 to 3 parts of polyether ester modified polysiloxane viscosifier. The invention further discloses a coating process and application of the TPU gluing silicone rubber heat transfer film. The process is simple, the cost is low, the production efficiency is high, the heat transfer film is easily machined, after-forming is achieved, weather resistance is good, hydrolysis resistance is achieved, the film is resistant to high and low temperature,good in color firming, easy to clean, and wide in silicone rubber thickness range, bubbles cannot appear inside, the property and the side effect after engraving of the silicone rubber heat transfer film cannot be affected, the cost is low, and the service life is long.