The invention discloses a locking glue array lead frame and application of the locking glue array lead frame in a chip packaging piece; a plurality of locking glue holes are uniformly formed in the surface of a substrate of the lead frame, and areas, without holes, among the locking glue holes are used for lead bonding. When the lead frame is applied, the application comprises the following steps:step 1, thinning a wafer of a chip, and then pasting a DAF film on the back surface; step 2, adhering the chip to the substrate of the lead frame; step 3, cleaning a circuit to be bonded, and carrying out lead bonding between the chip and the substrate, between the chip and pins, between the substrate and the pins, or between the chips to obtain a bonded circuit; and step 4, performing plasma cleaning on the bonded circuit, performing plastic package by a plastic package material, embedding the plastic package material into plastic locking holes to form a plastic package body, curing the plastic package body, then sequentially carrying out electrotinning, laser marking and rib cutting forming to obtain a single-chip package part. The plastic package material penetrates through the plasticlocking holes, the effects of pinning strengthening and separation layer spreading preventing are achieved, and the reliability of the product is improved.