39 results about "Glycine synthesis" patented technology

The invention belongs to the field of pharmaceutical chemical engineering intermediate production, which relates to a synthesis technology of p-hydroxyphenylglycine (HPG). The purpose of the invention is achieved by the following steps: phenol, glyoxylic acid, water and sulfamic acid carry out the one-pot braise reaction under the action of catalysts such as benzene sulfonic acid, p-toluenesulfonic acid, o-toluenesulfonic acid, and the like; after the reaction is finished, a small amount of reducing substances such as sodium sulfite, sodium bisulfite, hydroxylamine hydrochloride, and the like, are added; finally, the PH value is adjusted by alkali, the mother liquor separation is washed by large amount of water and then washed by organic solvents such as methanol, ethanol, acetone, glacial acetic acid, and the like; the obtained product HPG is white powder, wherein, the content of NPLC is equal to or more than 98.5, which can meet the requirement of splitting. The method has the advantages of low production cost, simple operation, stable quality, etc.

The invention relates to threonine aldolase, in particular to an L-threonine aldolase mutant R318M and application thereof. The amino acid sequence of the L-threonine aldolase mutant is shown as SEQ ID NO: 3, and the L-threonine aldolase mutant is obtained by changing the 318th amino acid of L-threonine aldolase with the amino acid sequence of SEQ ID NO: 1 from Arg to Met. The mutant has diastereoselectivity (de) of 77.1% in a reaction of catalyzing 3, 4-dihydroxy benzaldehyde and glycine to synthesize droxydopa [L-threonine-(3, 4-dihydroxy) phenylserine], the diastereoselectivity (de) is morethan 2.5 times that of a wild type, and the mutant has huge application potential in industrial production of biosynthesis of droxydopa.

The invention belongs to the field of pharmaceutical chemical engineering intermediate production, which relates to a synthesis technology of p-hydroxyphenylglycine (HPG). The purpose of the invention is achieved by the following steps: phenol, glyoxylic acid, water and sulfamic acid carry out the one-pot braise reaction under the action of catalysts such as benzene sulfonic acid, p-toluenesulfonic acid, o-toluenesulfonic acid, and the like; after the reaction is finished, a small amount of reducing substances such as sodium sulfite, sodium bisulfite, hydroxylamine hydrochloride, and the like, are added; finally, the PH value is adjusted by alkali, the mother liquor separation is washed by large amount of water and then washed by organic solvents such as methanol, ethanol, acetone, glacial acetic acid, and the like; the obtained product HPG is white powder, wherein, the content of NPLC is equal to or more than 98.5, which can meet the requirement of splitting. The method has the advantages of low production cost, simple operation, stable quality, etc.

The invention relates to a technology for treating phenol-contained wastewater in the synthetic process of L-(+)-D-p-hydroxyphenylglycine, which belongs to the technical field of the treatment of organic chemical wastewater. In the method, after the distillation pretreatment is carried out on synthesized wastewater, the macroporous resin adsorption treatment is carried out on the collected phenol-contained wastewater, wherein the pretreatment comprises the step of carrying out acidized pretreatment on the synthesized wastewater. In the method, because a phenol-contained substance in the wastewater is removed in advance, the recycling and processing difficulty of the subsequent inorganic salt can be greatly improved. The recycled sodium phenolate can be charged back to the synthesizing section of p-hydroxyphenylglycine. The method has stable and reliable treatment effect, low treatment cost and simple, convenient and easily-applied operation and is easy to realize industrialized application.

The invention relates to a method of catalytically synthesizing para hydroxybenzene hydantoin by solid acid. The method is characterized by synthesizing para hydroxybenzene hydantoin in the presence of a solid superacid catalyst SO4<2-> / ZrO2 / TiO2 or SO4<2-> / ZrO2 / SiO2. The technical scheme comprises synthesis of hydroxymandelic acid, synthesis of N-carbamyl-4-hydroxyl phenylglycine and synthesis ofpara hydroxybenzene hydantoin. The method not only solves the pollution problem of high concentration phenol waste acid and corrosion problem of production equipment, but also can control generationof o-hydroxybenzene hydantoin effectively, so that the synthetic yield of para hydroxybenzene hydantoin is improved.

The present invention relates to the process of treating glyphosate mother liquor from glycine synthesis process. The treating process includes the following successive steps: 1) introducing ammonia into glyphosate mother liquor to regulate pH to 7-10, letting stand and recovering triethylamine from the upper layer; and 2) decompression distilling lower layer of the mother liquor, cooling, filtering to eliminate ammonium chloride crystal and to obtain concentrated ammonium glyphosate liquid. The said treating process has low production cost, low power consumption and environment friendship, and can obtain concentrated liquid with raised effect. Furthermore, glyphosate may be separated from the mother liquor to raise total glyphosate yield.

The invention provides raw materials for synthesizing glycine and a method for improving glycine synthesis process. In the method, the glycine is synthesized by using industrial products of solid ammonium chloroacetate and ammonium bicarbonate as raw materials, water as a solvent and urotropine as a catalyst. The method has the advantages of lower reaction temperature, a few side reaction, a few impurities in the products of glycine, high quality and over 90 percent synthesis rate; and the method also has the advantages of cyclic utilization of mother liquor, less consumption of the catalyst, easy operation, high safety, low corrosiveness and little pollution.

γ-Glu-Val synthetase suitable for generating γ-Glu-Val, and a method for producing γ-Glu-Val-Gly using the same are provided. By using γ-Glu-Val synthetase showing a ratio of γ-glutamylvaline synthetase activity to γ-glutamylglycine synthetase activity of 2.0 or higher, such as γ-Glu-Val synthetase of Kocuria rosea (AJ3132), γ-Glu-Val-Gly is produced from Glu, Val, and Gly as raw materials.

γ-Glu-Val synthetase suitable for generating γ-Glu-Val, and a method for producing γ-Glu-Val-Gly using the same are provided. By using γ-Glu-Val synthetase showing a ratio of γ-glutamylvaline synthetase activity to γ-glutamylglycine synthetase activity of 2.0 or higher, such as γ-Glu-Val synthetase of Kocuria rosea (AJ3132), γ-Glu-Val-Gly is produced from Glu, Val, and Gly as raw materials.

The invention discloses a production process of glycine. The production process comprises the following steps of: S1, performing ammonification; S2, performing alkaline hydrolysis ammonia distillation; S3, performing decolorizing with activated carbon; S4, performing ion exchange acidification separation, specifically, performing ion exchange reaction on metal ions of discolored glycinate and H < + > on ion exchange resin to generate glycine, discharging the glycine out of a system along with an aqueous solution, performing ion exchange reaction on iminodiacetic acid disalt and the H < + > to generate iminodiacetic acid monosalt, adsorbing the iminodiacetic acid monosalt on the resin, discharging the iminodiacetic acid monosalt and an inorganic salt solution generated in a resin regeneration process out of the system; S5, concentrating and crystallizing glycine, and when the iminodiacetic acid monosalt in circulating mother liquor is accumulated to 10% or above of the total solute, pumping out the mother liquor, concentrating and crystallizing the inorganic salt, and when iminodiacetic acid monosalt in the solute of the circulating mother liquor is accumulated to 15% or above of the total solute, extracting the mother liquor; S6, mixing the extracted mother liquor, and carrying out continuous chromatographic separation; and S7, recycling the iminodiacetic acid. The production process of the glycine provided by the invention has good glycine synthesis and separation effects.

The present invention relates to a method for synthesizing glutathione using yeast cells. Gamma-GCS and GS in yeast cells are used, glucose is used as the energy, an intracellular glucolysis system is used to produce triphosadenine, glutamic acid, cysteine and glycin in reaction liquid are synthesized to form glutathione; saccharomyces cerevisiae, baker's yeast, candida and other yeast cells are permeabilized and then react in a reaction liquid composed of phosphate buffer, MgCl2.6H2O, CaCl2.2H2O, glucose, L-glutamic acid and L-cysteine, gamma-L-glutamoyl L- cysteine is produced, then glycin is added to produce glutathione; in the two-stage reaction, the inhabitation effect of glutathione to gamma-GCS catalytic reaction is greatly reduced; glutathione produced by the method has obviously improved product concentration and reaction yield.

The invention discloses a characteristic sequence for identifying Kumamoto oyster, specific identification primers and an identification method, wherein the characteristic sequence is located in the mitochondrial non-coding region between glycine tRNA synthetase and proline tRNA synthetase of oyster, and the characteristic sequence is The nucleotide sequence shown in SEQ ID NO.1; the sequence of the specific identification primer is: upstream primer MNR-F: 5'-CTGTAAGTATATTTGTCTTCCA-3'; downstream specific primer MNR-S-R: 5'-AGGCTTTCACTCCACTTACT ‑3'. The present invention can effectively distinguish Kumamoto oyster from other four species of Pyramid oyster, the determined characteristic sequence length of Kumamoto oyster is 660±5bp, and whether it is Kumamoto oyster can be judged by the existence and size of the specific band. In the field of Kumamoto oyster germplasm resource protection and Kumamoto oyster breeding, species identification can be carried out on broodstock before seed breeding to ensure that the breeding seed is pure and not affected by other species that may hybridize with it.

The invention discloses a recombinant Escherichia coli strain for producing 5-aminolevulinic acid (5-ALA); the invention also discloses a high-efficiency approach for synthesizing 5-aminolevulinic acid: strengthening the 5-aminolevulinic acid of Escherichia coli itself Aminolevulinic acid synthase (HemL and HemA), the strain initially has the ability to synthesize 5-aminolevulinic acid; strengthen the expression of the 5-aminolevulinic acid efflux protein eamA gene, and increase the 5-aminolevulinic acid efflux of the strain Removal ability; introduce exogenous 5-aminolevulinic acid synthase hemA gene to enhance the 5-aminolevulinic acid synthesis ability of the strain; transform galR, glk, ppc genes in the glucose utilization pathway to improve glucose utilization efficiency; knock out metabolism Bypassed genes (hemF, poxB, and aceB). The recombinant Escherichia coli strain constructed by the invention has the ability to efficiently utilize glucose and glycine to synthesize 5-aminolevulinic acid, making it useful for industrial production of 5-aminolevulinic acid.