The invention discloses a cotton fiber with beautify efficacy and a preparation method of the cotton fiber. The cotton fiber with beautify efficacy is characterized by being prepared by raw materials in parts by weight as follows: 25-35 parts of colored cotton fiber, 10-13 parts of linen fiber, 11-14 parts of tencel fiber, 7-9 parts of wood fiber, 4-7 parts of castor oil, 1-2 parts of potassium nitrate, 2.1-3.5 parts of nano TiO2, 1.1-2.3 parts of a softening agent VS, 10-15 parts of fatty acid methyl ester, 18-21 parts of ethylene glycol, 16-18 parts of dimethyl terephthalate, 5-7 parts of aloe, 2-5 parts of coptis chinensis, 3-4 parts of rhizoma imperatae, 100-120 parts of 1-butyl-3-ethylimidazolium dibutyl phosphate, 120-130 parts of 1-butyl-3-methylimidazole acetate, 4-7 parts of an auxiliary and an appropriate amount of water. According to the cotton fiber, the production technology and the formula are optimized, multiple types of animal and plant fiber are added, various performance indexes of natural cotton fiber are improved, and the cotton fiber has efficacy of beauty, bacterium prevention, mite prevention, smooth hand feeling and the like and can be widely applied to the field of beauty masks, beauty towel and the like.