The present application relates to the field of freeze-dried preparations of leeches, and provides a freeze-dried
powder containing multiple active enzymes and a preparation method thereof, to solve the problem that the prior art cannot simultaneously ensure the release of active ingredients and the maintenance of the stability of active ingredients, comprising the following steps: S100, cooling leeches to obtain freeze-dried leeches; S200, putting the freeze-dried leeches into a
solution system, crushing, centrifuging, and taking the supernatant; the pH value of the
solution system is 6.5-8.5; the
solution system at least comprises water; the crushing speed is 150-250 r / min, and the time is 5-8 min; S300, after the supernatant is separated and purified, freeze-
drying is performed to obtain the freeze-dried
powder of leeches. The present application adopts a freeze-crushing process, and after freezing, not only can the crushing operation be simply realized, but also the loss of active ingredients caused by high temperature can be reduced by utilizing the crushing process at low temperature of raw materials.