The invention discloses an organic electroluminescence device. The organic electroluminescence device comprises a substrate, a first electrode, a hole injection layer, a hole transmission layer, an organic light emitting layer, an electron transmission layer, an electron injection layer and a second electrode, wherein the substrate, the first electrode, the hole injection layer, the hole transmission layer, the organic light emitting layer, the electron transmission layer, the electron injection layer and the second electrode are sequentially stacked. The hole injection layer comprises two thin inorganic nano-rod films which are vertically stacked. The inclination angles of inorganic nano-rods of the thin inorganic nano-rod film at the side close to the substrate are smaller than the inclination angles of inorganic nano-rods of the thin inorganic nano-rod film at the side far away from the substrate. The thin inorganic nano-rod film with the smaller inclination angles is beneficial to the increase of the effective contact area between the hole injection layer and an adjacent electrode layer, the barrier height, from an anode to the hole injection layer, of a hole is effectively reduced, and the starting voltage and the working voltage are reduced. The thin inorganic nano-rod film with the larger inclination angles can effectively adjust the balance of electrons and the hole, the gap scattering property of the inclined nano-rods is used, so that on the premise that power efficiency is not sacrificed, the luminous efficiency is improved. The invention further discloses a preparing method of the organic electroluminescence device.