40results about How to "Solve the problem of lack" patented technology

The invention discloses total mixed pellet feed for meat sheep and a manufacturing method and application of the feed. The feed comprises: 20.00-40.00% of straw, 0.00-30.00% of caragana korshinskii, 0.00-30.00% of sunflower shells, 0.00-30.00% of leymus chinensis, 10.00-30.00% of corn, 5.00-10.00% of molasses, 5.00-8.00% of soybean meal, 0.00-10.00% of cottonseed meal, 0.00-5.00% of sesame meal, 0.00-15.00% of maize germ meal, 0.00-5.00% of rapeseed meal, 0.00-8.00% of corn bran with slurry, 0.00-3.00% of wheat bran, 0.00-5.00% of rice bran, 0.00-20.00% of corn distiller's dried grains with soluble, 0.00-3.00% of bentonite, 0.40-0.80% of salt, 0.10-0.50% of calcium hydrogen phosphate, 0.50-1.50% of stone flour, 0.00-0.80% of slow-release protein, 0.50-1.50% of sodium bicarbonate, 0.04-0.08% of a mildew inhibitor, 0.40-0.50% of ammonium chloride, 1.00-3.00% of Chinese herbal medicine premix and 0.80-1.50% of meet goat fattening premix. The feed can replace common refined feeding and roughage, the breeding process is simplified, the breeding cost is saved, and the total mixed pellet feed can prevent the occurrence of rumen acidosis and urinary calculus of meet sheep.

The invention discloses an occluded pedestrian re-identification method based on massed learning and deep network learning. According to the method, multiple types of occluded training samples are generated from original non-occluded training samples through an occlusion simulator, the generated occluded training samples and the original training samples form a united training set used for model training, meanwhile, occluded and non-occluded classification losses are added into pedestrian classification losses, and a multi-task loss function is used to replace a single-task loss function in the past. In this way, pedestrian re-identification under occlusion is effectively coped with, and priori information of occlusion and non-occlusion is considered to perform feature extraction during deep network feature learning. Experiments indicate that through the method, the performance of an existing deep network in occluded pedestrian re-identification can be substantially improved, and the method has high application value.

The invention discloses polynucleotide, a method and a kit for detecting listeria monocytogenes. Specifically, the invention discloses polynucleotide, which can be used as a standard molecule in realtime fluorescent PCR detection of listeria monocytogenes, and DNA constructs (LY01 and LY02). The provided plasmid standard molecule can solve the problem that realtime fluorescence PCR detection of listeria monocytogenes is lack of standard substances, and moreover, can guarantee the comparability of the detection results of realtime fluorescence PCR detection. A reliable quality control method is provided for the realtime fluorescence PCR detection of listeria monocytogenes.

The invention relates to a high-powered recycling process of coal-chemical industry clean wastewater and a special device for the high-powered recycling process. The coal-chemical industry clean wastewater is subjected to multistage clarification, ultrafiltration and osmosis treatment, the water recovery rate of the clean wastewater ultimately reaches above 95%, the purpose of high-powered recycling of the coal-chemical industry clean wastewater is achieved, the water resource is saved and the difficult problem of the deficiency of the fresh water resource is solved.

The invention discloses a polynucleotide, a method and a kit for detecting Bacillus anthraci, and particularly discloses the polynucleotide which can be used as a standard molecule for real-time fluorescent PCR (polymerase chain reaction) detection of the Bacillus anthraci as well as DNA constructs PA and capA. By the aid of the plasmid standard molecule, the problem of lack of standard substances for real-time fluorescent PCR detection of the Bacillus anthraci is solved, the comparability of the detection result of a real-time fluorescent PCR method of the Bacillus anthraci is guaranteed, and a reliable quality control method is provided for detection with the real-time fluorescent PCR method of the Bacillus anthraci.

The invention provides a selenium-rich high-dietary-fiber recombinant rice and a processing method thereof. The processing method comprises the following steps: uniformly mixing the following raw material powder in parts by weight, adding water which accounts for 20-35% of the weight of the mixed raw material powder for hardening and tempering, putting the mixed raw material powder into a double-screw extrusion granulator for low-temperature extrusion to form rice grains, and carrying out air draft drying at 35-65 DEG C for 1-2 hours to obtain the selenium-rich high-dietary-fiber recombinant rice with the dietary fiber content of 6-15% and the selenium content range of 18-30mu g/100g. The mixed raw material powder is prepared from the following raw material powder in percentage by weight: rice flour, whole grain powder, medicinal and edible food powder, bean powder, potato powder, dietary fiber powder and a selenium-enriched oyster mushroom enzyme hydrolysis extract. The selenium-rich high-dietary-fiber reprocessed rice can well solve the problem of a supplement source of selenium element for people in selenium-deficient areas, and can be used as a supplement source of dietary fibers for people with insufficient dietary fiber intake, so as to achieve the purpose of supplementing the dietary fibers.

The invention discloses a polynucleotide, method and kit for detecting transgenic crops. Specifically, the present invention discloses the sequences of the polynucleotide construct pLW10 and its matching primer probes that can be used as standard molecules for real-time fluorescent PCR detection of transgenic crops. The plasmid standard molecule of the present invention solves the problem of lack of standard substances in the real-time fluorescent PCR detection of transgenic crops, ensures the comparability of the detection results of the real-time fluorescent PCR method of the transgenic crops, and provides a reliable quality control method for the real-time fluorescent PCR detection of the transgenic crops.

The invention discloses a plasmid standard molecule of enterobacter sakazakii, a preparation method and a quantification method of the plasmid standard molecule and applications thereof. An obtained standard substance is a plasmid standard molecule pXL04, wherein the sequence of the plasmid standard molecule pXL04 is represented by SEQ ID NO: 2 in a sequence table. According to the plasmid standard molecule of enterobacter sakazakii, the plasmid standard molecule pXL04 is quantified by using an ultraviolet spectrophotometric method and a high-resolution inductive coupling plasma emission mass spectrum (HR-ICP-MS) so as to ensure the good traceability. According to the plasmid standard molecule, the difficulty that the standard substances are lacked in real-time fluorescence quantification PCR detection of the enterobacter sakazakii is overcome; the comparability of a detection result of a real-time fluorescence quantification PCR detection method of the enterobacter sakazakii is ensured and a reliable quality control method is provided for the detection of the real-time fluorescence quantification PCR detection method of the enterobacter sakazakii.

The invention discloses a plasmid standard molecular adaptable to sakazakii real-time fluorescent quantitation PCR (polymerase chain reaction) detection, a preparation method thereof, a quantitation method thereof and application. The plasmid standard molecular comprises a 16S rDNA gene sequence of the sakazakii. The 16S rDNA gene sequence is shown as SEQ ID NO: 1 in sequence lists. The plasmid standard molecular is quantitated by the ultraviolet light spectrophotometry method and a high resolution inductively coupled plasma emission mass spectrometry (HR-ICP-MS), and good traceability is guaranteed. By the aid of the plasmid standard molecular, the problem of the lack of standard material during the sakazakii real-time fluorescent quantitation PCR (polymerase chain reaction) detection, comparability of results of the sakazakii real-time fluorescent quantitation PCR detection, and a reliable quality control method is provided for the sakazakii real-time fluorescent quantitation PCR detection.

The invention discloses a standard molecule for detecting tilletia walkeri castebury&carris;. The standard molecule is a plasmid which can be duplicated, and the plasmid contains at least one of a sequence A (Seq ID N0.1) and a sequence B (Seq ID N0.2); the sequence A is 99.65% homologous with a 2.3kb nucleotide sequence of the mitochondrial DNA of the tilletia walkeri castebury&carris; and the sequence B is 100% homologous with the DNA sequence of a ribosomal internal transcribed spacer of the tilletia walkeri castebury&carris;. The invention also discloses a preparation method and detection method of the standard molecule. The standard molecule constructed in the invention can serve as a positive control for detecting the molecule of the tilletia walkeri castebury&carris; instead of the DNA of the tilletia walkeri castebury&carris; is suitable for qualitative and quantitative PCR (polymerase chain reaction) detection of the tilletia walkeri castebury&carris; and is suitable for the inspection and quarantine department of the port, the agricultural production department, the plant protection department and the like.

The invention discloses C80 concrete prepared from stone chips, the preparation materials of the concrete comprise a cementing material, coarse aggregate, a water reducing agent, water and high-calcium stone chips, the dosage of the cementing material is 580-600kg/m < 3 >, and the water-binder ratio is 0.22-0.26. The C80 concrete has the beneficial effects that the stone chips with the average particle size of less than 4.75 mm are adopted, and stone powder contained in the stone chips can fill cement particle gaps; the gel compactness is improved, the surface energy is relatively low, the dispersion is easy, and the water requirement for achieving the same liquidity is low; the problem of lack of high-quality fly ash can be solved by blending ultrafine stone powder in the stone chips andslag powder to prepare high-performance concrete; river sand is replaced with the stone chips of mine production stone to serve as fine aggregate, the problem that no high-quality river sand is used for preparing high-strength concrete in mountainous areas is solved, environmental protection and sustainable development of building materials are facilitated, and huge economic benefits are achieved.

The invention aims at providing a multifunctional portable moxibustion apparatus system. The system solves the problem of lack of professional moxibustion technicians. A user can select various moxibustion modes at home by himself/herself through the intelligent operation of the moxibustion apparatus system, and a series of moxibustion methods such as hot moxibustion, warm moxibustion and comfortable moxibustion can be implemented by setting time and controlling the temperature and time.

The invention discloses polynucleotide, a method and a kit for real-time fluorescence quantitative PCR (Polymerase Chain Reaction) detection of vibrio cholerae, and particularly provides the polynucleotide, usable for vibrio cholerae PCR detection, and a primer pair matched with the polynucleotide. A polynucleotide sequence is prepared into a standard plasmid molecule PLW07 by using an appropriate framework plasmid, and real-time fluorescence PCR detection is carried out by virtue of the primer pair; the specificity and the sensitivity are extremely high, and the stability is high.

The invention discloses polynucleotide for staphylococcus aureus detection, a method and a kit and particularly discloses the polynucleotide of standard molecules and capable of being used for real-time fluorescence PCR detection of staphylococcus aureus and a DNA construct LY03. According to the polynucleotide for staphylococcus aureus detection, the method and the kit, plasmid standard molecules solve the problem that standard matter lacks in real-time fluorescence PCR detection of the staphylococcus aureus, the result comparability of the real-time fluorescence PCR method detection of staphylococcus aureus is guaranteed, and a reliable quality control method is provided for real-time fluorescence PCR method detection of staphylococcus aureus.

The invention relates to pickled finger citron. The pickled finger citron is prepared in steps as follows: (1), peeling, shredding and microwave vacuum drying of ripe finger citron; (2), flavoring; (3), fermentation; (4), subpackaging. Pulp of the finger citron is processed according to the comprehensive medicine efficacy of the finger citron and the medicine and food homology concept to form the pickled finger citron, and the problem of lack of finger citron products on the market is solved.