40results about How to "Solve the problem of lack" patented technology

The invention relates to a method for promoting growth of plants on manganese tailing residues. Used indigenous bacteria come from the tailing residues on which plants do not grow. Used plants are Phytolacca americana. An inoculated water-retaining gel bacteria bed and the tailing residues are evenly mixed according to a mass ratio being 1: (4-8) to cultivate Phytolacca americana seedlings. The plants can be promoted to grow and breed on the manganese tailing residues to form vegetation. The water-retaining gel bacteria bed contains 5 parts of white mica, 5 parts of montmorillonite, 7 parts of agar and 83 parts of indigenous bacteria culture solution. The indigenous bacteria culture solution contains soluble starch with concentration being 5g / L, beef tallow cream with concentration being 1g / L, peptone with concentration being 3g / L, dipotassium hydrogen phosphate with concentration being 0.2g / L, sodium chloride with concentration being 5g / L, cane sugar with concentration being 3g / L, magnesium sulfate with concentration being 0.2g / L, ferrous sulfate with concentration being 0.2g / L, potassium nitrate with concentration being 0.5g / L, urea with concentration being 10g / L and calcium superphosphate with concentration being 5g / L. The method has the advantages that the operation is simple, the cost is low, the efficiency of resisting and controlling the migration and the diffusion of manganese and other harmful metal in manganese tailing residues, the environment can be beautified and the like.

The invention discloses a polynucleotide, a method and a kit for detecting Bacillus anthraci, and particularly discloses the polynucleotide which can be used as a standard molecule for real-time fluorescent PCR (polymerase chain reaction) detection of the Bacillus anthraci as well as DNA constructs PA and capA. By the aid of the plasmid standard molecule, the problem of lack of standard substances for real-time fluorescent PCR detection of the Bacillus anthraci is solved, the comparability of the detection result of a real-time fluorescent PCR method of the Bacillus anthraci is guaranteed, and a reliable quality control method is provided for detection with the real-time fluorescent PCR method of the Bacillus anthraci.

The invention discloses a method for organizing the mixing of spray jet and mainstream gas. By analyzing the mixing mechanism and law of spray jet and mainstream gas, combined with particle dynamics, vortex dynamics and other theories, a blending method based on regulation and control is proposed. The large-scale vortex structure-symmetric anti-vortex pair that dominates the diffusion characteristics of spray droplets in the flow field is used to realize a new idea of ​​rationally organizing the mixing process of the spray jet and the mainstream gas, and according to the characteristics of the symmetrical anti-vortex pair in the mixing flow field The internal relationship between the size and the parameters of the flow field, the determination method of the spray jet atomization characteristic parameters and the nozzle selection method based on the mainstream gas state are given, which solves the problem of mixing the spray jet with the mainstream gas in the current industrial process. The problem of lack of organizational skills in the mixed process.

The invention relates to a high-powered recycling process of coal-chemical industry clean wastewater and a special device for the high-powered recycling process. The coal-chemical industry clean wastewater is subjected to multistage clarification, ultrafiltration and osmosis treatment, the water recovery rate of the clean wastewater ultimately reaches above 95%, the purpose of high-powered recycling of the coal-chemical industry clean wastewater is achieved, the water resource is saved and the difficult problem of the deficiency of the fresh water resource is solved.

The invention discloses a method for preparing a Chinese poplar or sycamore or pine wood and plastic composite material by adopting the radiation polymerization, crosslinking and grafting jointly. The method has the following steps of: pre-treatment, monomer impregnation, constant temperature irradiation under the protection of nitrogen and post-treatment and specifically comprises the following steps: (1) preparing the Chinese poplar or sycamore or pine wood serving as the basic material into blanks with appropriate sizes, drying in a drying room, and putting the treated blanks in an impregnating and radiating product box; (2) impregnating by methyl methacrylate, other acrylic acid derivatives and related assistants, introducing protection nitrogen, and increasing the pressure, wherein the impregnation rate of a monomer is 40-170%; (3) radiating at the room temperature under the nitrogen protection, wherein the absorbed dose is 15kGy; and (4) performing aftertreatment: polishing the surface of a radiated wood and plastic composite material to prepare for processing the composite material to floor boards and other products. The fast-growing readily-available low-grade wood such as the Chinese poplar wood is used for preparing the wood and plastic composite material by adopting the radiating, the crosslinking and the grafting, and the wood and plastic composite material has high hardness, good wear resistance, low hygroscopicity and corrosion resistance, can be used for replacing the expensive high-quality wood so as to meet various use requirements.

The invention discloses polynucleotide, a method and a kit for real-time fluorescence quantitative PCR (Polymerase Chain Reaction) detection of vibrio cholerae, and particularly provides the polynucleotide, usable for vibrio cholerae PCR detection, and a primer pair matched with the polynucleotide. A polynucleotide sequence is prepared into a standard plasmid molecule PLW07 by using an appropriate framework plasmid, and real-time fluorescence PCR detection is carried out by virtue of the primer pair; the specificity and the sensitivity are extremely high, and the stability is high.

The invention discloses plasmid standard molecules PLW03 and PLW04 for detecting Shiga-toxin-producing Escherichia coli and application thereof. The invention particularly discloses a polynucleotide for Shiga-toxin-producing Escherichia coli PCR detection and a primer pair matched with the polynucleotide. The polynucleotide sequence is prepared into the standard plasmid molecules by using an appropriate framework plasmid. When being matched with the primer pair to perform real-time fluorescent PCR detection, the standard plasmid molecules have the advantages of excellent specificity, excellent sensitivity and favorable stability.

The invention discloses a standard molecule for detecting tilletia walkeri castebury&carris;. The standard molecule is a plasmid which can be duplicated, and the plasmid contains at least one of a sequence A (Seq ID N0.1) and a sequence B (Seq ID N0.2); the sequence A is 99.65% homologous with a 2.3kb nucleotide sequence of the mitochondrial DNA of the tilletia walkeri castebury&carris; and the sequence B is 100% homologous with the DNA sequence of a ribosomal internal transcribed spacer of the tilletia walkeri castebury&carris;. The invention also discloses a preparation method and detection method of the standard molecule. The standard molecule constructed in the invention can serve as a positive control for detecting the molecule of the tilletia walkeri castebury&carris; instead of the DNA of the tilletia walkeri castebury&carris; is suitable for qualitative and quantitative PCR (polymerase chain reaction) detection of the tilletia walkeri castebury&carris; and is suitable for the inspection and quarantine department of the port, the agricultural production department, the plant protection department and the like.