The invention discloses fruit and vegetable cereal protein powder and a preparation method thereof. The fruit and vegetable cereal protein powder is prepared from the following raw materials in parts by weight: 200-210 parts of millets, 40-42 parts of bananas, 5-6 parts of pawpaw, 4-5 parts of pomegranate juice, 4-5 parts of sesame oil, 4-5 parts of rye flour, 24-25 parts of gynura, 5-6 parts of pumpkins, 12-14 parts of sesames, 10-12 parts of pistachio nuts, 1-2 parts of chicken's gizzard-membrane, 2-3 parts of loofah sponge, 1-2 parts of poria cocos, 3-4 parts of Chinese waxgourd peel, 1-2 parts of dried tangerine or orange peel, 2-3 parts of jar grass, 1-2 parts of viola odorata and 6-7 parts of a nutrient solution. The protein powder is added with various fruits and vegetables, so that the taste is sweet and the flavor can be improved remarkably; and moreover, nutritional ingredients such as vitamins and celluloses are rich in the vegetables and fruits, and various Chinese herbal medicines are matched to facilitate intestines and stomach, and thus, the fruit and vegetable cereal protein powder is suitable for being eaten by various people.