The invention discloses grape flavor mixed fruit jam, which is prepared from the following raw materials in parts by weight: 100 to 300 parts of purple skin grapes, 100 to 200 parts of green skin grapes, 50 to 100 parts of apples, 50 to 100 parts of bananas, 30 to 80 parts of pineapples, 30 to 50 parts of pawpaws, 20 to 50 parts of dragon fruits, 100 to 200 parts of mashed purple sweet potato, 50 to 100 parts of mashed pumpkin, 20 to 50 parts of honey, 5 to 10 parts of white granulated sugar, 20 to 30 parts of white sesame powder and 20 to 30 parts of black sesame powder. In addition, the invention also discloses a preparation method for preparing the grape flavor mixed fruit jam. The grape flavor mixed fruit jam contains the grapes, the apples, the bananas, the pineapples, the pawpaw and the dragon fruits; the fruit type is rich; the mouthfeel is mellow; the purple sweet potato, the pumpkin, the white sesame powder and the black sesame powder are also added; the plant fiber and plant protein are added, so that the nutrition of the jam is more comprehensive; the better health care effects are achieved; in addition, a great number of grapes are used; the grape deep processing problem is solved.