Ruthenium is a transition metal element, has multiple valence states and complicated coordination states and can form various organic complexes. The bivalent ruthenium organic compound is the most common, and researches on the trivalent and the nulvalent ruthenium organic complexes are fewer. [C6Me6]2Ru is similar to ferrocene in shape and has a bread sandwich structure. E.O.Fischer firstly reports the synthesis of the compound [C6Me6]2Ru; aluminum powder and diethyl aluminum chloride used in the reaction have active chemical properties and can be oxidized and even burn and explode in air; and aluminum trichloride and aluminum tribromide are very sensitive to water. A Fischer [C6Me6]2Ru synthesis technology comprises four steps, wherein three steps need to be performed in an anhydrous and oxygen-free condition, reactants and reagents need to be dehydrated and deoxidized, the safety coefficient of a preparation process is low and the total yield is lower and is 19% only (in terms of RuCl3). The invention develops a brand new synthesis design way, in which RuCl3 hydrate is adopted as a raw material, four steps are performed for synthesis and reaction to prepare the [C6Me6]2Ru; the yield of the novel process reaches 39%, which is improved by 20% compared with that of the E.O.Fischer synthesis technology, and the reagents used in the synthesis process are stable and readily available; except for the last step of sodium ammonia reduction, dehydration and deoxidization are not needed in the other three steps, therefore, the operation technology process is simplified, the safety coefficient is raised, and the novel process is suitable for the industrialized production. The organic metal Ru(O) complex which is wide in application range is a potential homogeneous catalyst, can be applied to catalysis of aromatic hydrogenation and asymmetrical catalysis, and can also be applied to the field of clinical medicaments to serve as a novel anti-cancer medicament.