The invention discloses a sample preparation method for analysis samples of bamboo shoot flesh. The method comprises the following steps: selecting raw material, cleaning, removing roots, drilling for sampling, chopping, mixing, freezing, packaging, and refrigerating to obtain the analysis samples of the bamboo shoot flesh. According to the method provided by the invention, sample points with different aging degrees and different physiological activities can all be obtained, drawbacks of the existing sample preparation are overcome, the problems of heterogeneity of growth of the bamboo shoots, poor representativeness of the traditional sampling methods and large deviation of reliability of comparison between batches are solved, the representativeness and accuracy of asymmetrical bamboo shoot flesh samples are greatly improved, system errors are reduced, and the reliability and accuracy of the analysis results can be improved. The method plays a huge role in the biological analysis of bamboo shoots, and is particularly applicable to the analysis of nutrient composition of bamboo shoots, analysis of heavy metal contents, analysis of pesticide residues, analysis of antibiotic contents, postharvest physiological and biochemical analysis, DNA or RNA extraction analysis and sampling.