The invention relates to the technical field of
bacterial cellulose preparation, in particular to a composite
flora for producing
bacterial cellulose and a preparation method thereof, and the preparation method comprises the following steps: screening
gluconobacter oxydans, obtaining culture medium preparation materials and preparation tools, preparing a slant culture medium set, preparing an enriched
liquid culture medium set, and preparing composite
flora mixed
inoculation liquid. The method comprises the following steps: preparing a waste
tobacco leaf leaching solution, extracting a proportional leaching solution from the waste
tobacco leaf leaching solution according to a preset proportion, obtaining a mixed
fermentation culture medium based on a preparation tool and the proportional leaching solution, and carrying out
inoculation operation on a composite
flora mixed
inoculation solution by using the mixed
fermentation culture medium to obtain a dry
cellulose membrane; calculating the yield of the
bacterial cellulose based on the dry
cellulose membrane, and completing the preparation of the composite flora for producing the bacterial
cellulose based on the yield of the bacterial cellulose. The method can be used for scientifically, efficiently and stably preparing the composite flora.