The invention belongs to the technical field of aquatic feed, and particularly relates to breeding feed for larimichthys crocea fries and a preparation method of the breeding feed. According to the invention, the puffed corn flour, the puffed soybean flour, the puffed tapioca flour and the puffed rice flour are added according to a specific proportion, so that the purposes that main nutritional ingredients in the feed are balanced and are easy to digest and absorb are achieved. According to the invention, a preheating type screw bulking machine is also utilized to carry out specific and sufficient bulking operation on the four crushed materials, so that the basic advantages of sterilization, food calling and easy absorption of the bulked powder are realized. The invention has the following advantages: 1, four kinds of puffed powder have basic advantages of high-temperature sterilization, good flavor, high food calling property, easy digestion and easy absorption of nutrient substances; secondly, four kinds of puffed powder are added according to a specific proportion, so that the proportion of main nutrient substances such as starch, grease, vitamins and protein is suitable for breeding the larimichthys crocea fries;