The invention discloses a slow-release filler and a preparation method thereof, which belongs to the technical field of sewage treatment, the sustained-release filler comprises a first nylon skeleton,a second nylon skeleton, rotary biofilm culturing groups, a carbon source and a coating film, the first nylon skeleton is detachably matched with the second nylon skeleton; a spherical skeleton is formed after assembly; the rotary biofilm culturing groups are detachably and rotatably connected into the first nylon skeleton and the second nylon skeleton; wherein the first nylon skeleton, the second nylon skeleton and the rotary biofilm culturing group are respectively provided with a plurality of accommodating holes communicated with the outside, the accommodating holes are filled with carbonsources, and orifices of the accommodating holes are sealed by the coating films, the preparation method of the slow-release filler comprises the following steps: S1, injection molding, S2, filling the carbon sources, S3, curing the carbon sources, and S4, assembling. According to the slow-release filler and the preparation method thereof, the carbon source utilization rate is high, the biofilm culturing capacity is high, and falling of aged biofilms is facilitated.