The invention belongs to the technical field of feed, and in particular relates to a compound feed for layer breeder hens during the laying period which improves the healthy chick rate, and to a preparation method of the feed. The compound feed is mainly prepared from the following raw materials in parts by weight: 50-65 parts of cereals, 15-25 parts of soybean meal, 1-5 parts of corn gluten meal, 1-6 parts of rice bran meal, 2-6 parts of alfalfa powder, 1-3 parts of vegetable oil, 0.35-0.7 parts of amino acids, 0.05-0.1 parts of complex enzymes, 0.05-0.2 parts of prebiotics, 0.01-0.03 parts of 25-hydroxycholecalciferol, 0.01-0.3 parts of β-carotene, compound organic trace elements 0.03‑0.08 part, 1 part of compound premix. The sufficient and effective energy, amino acid, mineral nutrition and prebiotics of the present invention can ensure the requirement of maximum laying potential of layer breeder hens, high egg production rate and suitable egg weight, and high healthy chick rate of layer breeder hens.