The invention discloses a preparation method of an anti-static bacteriostatic 
polyester staple 
fiber, belonging to the technical field of 
polyester staple fibers. The preparation method comprises thesteps of carrying out high-temperature anaerobic 
sintering on bamboo chips, so as to obtain 
activated carbon; blending and compounding the 
activated carbon with 
polyester, so as to generate an artificial 
fiber; and blending and stretching a natural mulberry 
fiber with the artificial fiber during traction and wiredrawing, so as to obtain the anti-static bacteriostatic polyester staple fiber. The preparation method has the beneficial effects that the smooth execution of a subsequent 
processing process of 
textile is promoted; the adopted 
activated carbon is prepared by 
sintering the bamboo chipsunder high-temperature anaerobic conditions, bamboos are acidic, the prepared activated carbon is acidic, and the growth and propagation of 
bacteria under the acidic condition are inhibited, so that abacteriostatic effect is achieved; and relatively deep stripes are longitudinally distributed on the surface of the mulberry fiber, the surface comprises a middle cavity structure and more micro-holes, the activated carbon is inserted into the micro-holes of the mulberry fiber, the interfacial 
adhesive force between the mulberry fiber, and the polyester fiber is increased by virtue of the activated carbon, and a durable bacteriostatic effect is achieved, so that the bacteriostatic activity of the polyester staple fiber is enhanced, and the polyester staple fiber has wide application prospectsin the 
textile field.